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Multifunctional light-absorbing, scattering and transmitting spectrograph based on surface plasma wave

A technology of surface plasmon waves and spectrometers, which is applied in the direction of scattering characteristic measurement, color/spectral characteristic measurement, instruments, etc., can solve the problems of inability to realize multi-spectrum correlation, low scalability, and not involving Rayleigh scattering spectrum detection, etc.

Inactive Publication Date: 2007-11-21
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above devices are all single-connected systems with low scalability and cannot realize the correlation of various spectra, and none of them involve the detection of Rayleigh scattering spectra

Method used

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  • Multifunctional light-absorbing, scattering and transmitting spectrograph based on surface plasma wave
  • Multifunctional light-absorbing, scattering and transmitting spectrograph based on surface plasma wave
  • Multifunctional light-absorbing, scattering and transmitting spectrograph based on surface plasma wave

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Experimental program
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Effect test

Embodiment 1

[0058] The basic structure of the multifunctional light absorption, scattering and emission spectrometer based on surface plasmon waves of the present invention is shown in FIG. 1 . Among them, 11 is the light source, selected according to the needs of the test, which can be semiconductor laser, He-Ne laser, dye laser, halogen tungsten lamp, xenon lamp, Nernst lamp, silicon carbide rod lamp or continuous wavelength light-emitting diode; 12 is incident light modulation system; 13 is an electromagnetic coupling reaction system, in which the prism is an example of a common isosceles right-angled triangular prism; 14 is a temperature control system; 15 is an emitted light detection system, 16 is a reflected light detection system, and 17 is a data acquisition and management system , 18 are microcomputers.

[0059] Data acquisition and management system: This part is mainly composed of data acquisition card, data processing and analysis software and computer, which can complete tas...

Embodiment 2

[0064] Since scattered light detection is the most difficult to implement among the fluorescence, Raman, scattering, and surface plasmon resonance spectra, an example of the collection of scattered signals is given here emphatically. A 50nm-thick gold film was coated on the bottom of the prism as a sensing film, a He-Ne laser was used as a light source, a photomultiplier tube was used as a scattered light detector, and the iodide-rhodamine B ion association system was used as a probe. Concentration of KIO 3 taking the test. The flow injection analysis results are shown in Figure 7. Determination of KIO 3 The minimum detection limit was 100 nmol / L.

Embodiment 3

[0066] The same light source, detector and resonance light scattering probe as in Example 2 were used. A 50nm-thick gold film is coated on the bottom of the prism as a highly reflective sensing film, and the sensing film is chemically modified to inhibit the non-specific adsorption of samples on the surface of the sensing film. Under these conditions, the concentration analysis of Cr(VI) was carried out. The optimized angle diagram is shown in Figure 8, and Figure 9 shows the results of flow injection analysis of different concentrations of Cr(VI). The minimum detection limit of Cr(VI) by this method is 40 nmol / L.

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Abstract

A multifunction spectrograph based on surface plasma wave consists of light source, modulation unit of incoming light, modulation unit of angle, sample conveying unit, sample electromagnetic coupling reactor, detection unit of emission light, detection unit of reflection light, data collection / management unit and temperature control unit. It is featured as coupling and resonating plasma at surface of incoming-light wave resultant sensing film by said spectrograph for making fade field at surface of said sensing film be intensified so as to excite scattered light by utilizing intensified fade field.

Description

technical field [0001] The invention belongs to the field of biological analysis experiment equipment, relates to light scattering, surface plasmon resonance, fluorescence spectrum, Raman spectrum and other technologies, and is a new technology for combining surface plasmon resonance and multi-spectrum. Background technique [0002] Surface plasmon resonance spectroscopy (surface plasmon resonance, SPR) is an effective method to study the adsorption process of molecules on solid surfaces. The method has the advantages of real-time dynamic analysis, label-free detection, and high selectivity. In principle, when the incident light and the surface plasmon resonate under appropriate conditions, the intensity of the evanescent wave near the interface can be increased by 1 to 2 orders of magnitude. This enhanced vanishing field can be used to excite emission spectra such as light scattering, fluorescence, and Raman spectroscopy, thereby realizing dark background spectrum detectio...

Claims

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Application Information

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IPC IPC(8): G01N21/00G01N21/41G01N21/31G01N21/63G01N21/47
Inventor 陈义韩志强齐莉
Owner INST OF CHEM CHINESE ACAD OF SCI
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