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Separation and purification method for skeletal muscle satellite cells in goats

A satellite cell, separation and purification technology, applied in the field of separation and purification of goat skeletal muscle satellite cells

Inactive Publication Date: 2016-06-08
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, there are two main methods for isolating skeletal muscle satellite cells: enzymatic method and tissue block method, but each has advantages and disadvantages.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0016] This embodiment is a method for separating and purifying goat skeletal muscle satellite cells, comprising the following steps:

[0017] 1. Isolation of goat skeletal muscle satellite cells

[0018] (1) First, sterilize and sterilize the test benches and equipment used in the laboratory, and prepare corresponding reagents, including PBS containing double antibodies, normal saline containing double antibodies, cell culture medium, etc.

[0019] (2) collect the muscle tissue of isolated Huanghuai goat, fully clean and disinfect with 75% alcohol earlier, wash the muscle tissue with normal saline containing penicillin and streptomycin, wherein the concentration of penicillin and streptomycin is respectively 500IU / ml and 500μg / ml, then remove the fat and connective tissue, wash away the blood stains and stains on the surface as much as possible, cut it into 0.5×0.5cm tissue pieces, and separate the primary cells within two hours.

[0020] (3) After sending the retrieved tis...

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PUM

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Abstract

The invention discloses a separation and purification method for skeletal muscle satellite cells in goats. The separation and purification method comprises separation of the skeletal muscle satellite cells in the goats and purification of the skeletal muscle satellite cells in the goats. According to the method, trypsin digestion and a tissue mass method are combined for separation of the skeletal muscle satellite cells, a differential adhesion method is used continuously for purification of the skeletal muscle satellite cells in the goats during cell passage and is used for purification of the skeletal muscle satellite cells in the goats during more than 5 successional generations in late passage process, and the skeletal muscle satellite cells with higher purity are obtained.

Description

technical field [0001] The invention relates to a method for separating and purifying goat skeletal muscle satellite cells. Background technique [0002] my country has abundant goat breed resources, but due to the influence of reproductive rate and growth performance, there is still a lot of room for improvement in goat breeding in my country. With the continuous improvement of national living standards, goat meat is favored by more and more consumers due to its rich nutrition and delicious meat quality. [0003] Skeletal muscle satellite cells were first discovered by Mauro in 1961. They are muscle-derived stem cells with proliferation and differentiation capabilities located between the muscle cell membrane and the basement membrane. Later, it was further confirmed that muscle satellite cells participated in the growth of skeletal muscle. It has the ability of self-proliferation and multi-directional differentiation, maintains the stability of the muscle stem cell pool i...

Claims

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Application Information

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IPC IPC(8): C12N5/077
CPCC12N5/0658C12N2509/00
Inventor 凌英会王康岩朱龙章孝荣张运海李运生吴昊睢梦华郑琪
Owner ANHUI AGRICULTURAL UNIVERSITY
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