The invention discloses a technology for producing
lycopene by biological
fermentation and relates to the field of biological
pharmacy. The technology for producing the
lycopene by the biological
fermentation, disclosed by the invention, comprises the following steps: inoculating positive
fungus spores and negative
fungus spores into
saline solutions respectively to obtain a positive
fungus sporesuspension solution and a negative fungus
spore suspension solution; inoculating the positive fungus
spore suspension solution and the negative fungus
spore suspension solution into a seed culture medium according to the
concentration ratio of 2 to (3 to 5) to obtain a seed solution; inoculating the seed solution into a
fermentation culture medium and carrying out fermentation culture; after carrying out the fermentation culture for 12h, supplementing a fermentation culture medium which is equivalent to an original fermentation culture medium into a fermented solution. By adopting the technology disclosed by the invention, a form of the seed solution is improved; the positive spore suspension solution and the negative spore suspension solution are directly inoculated into the seed culturemedium to carry out conjugation; produced thalli are extremely small fungus
pellets or thalli and pelletized thalli are not generated, so that the absorption of
oxygen and
nutrient substances in a
liquid culture medium is facilitated; fermentation conditions are improved so that the
metabolism of the thalli and the expression activity of a product are enhanced; the accumulation of the
lycopene isfacilitated and the yield of the lycopene is improved.