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Method for promoting myogenic differentiation of bovine skeletal muscle satellite cells

A satellite cell, myoblast differentiation technology, applied in the field of biology, cell and tissue engineering

Active Publication Date: 2017-10-24
TIANJIN AGRICULTURE COLLEGE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are few reports on lncRNAs in the myogenic differentiation of bovine muscle satellite cells

Method used

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  • Method for promoting myogenic differentiation of bovine skeletal muscle satellite cells
  • Method for promoting myogenic differentiation of bovine skeletal muscle satellite cells
  • Method for promoting myogenic differentiation of bovine skeletal muscle satellite cells

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Embodiment Construction

[0016] The implementation of the present invention will be described in further detail below in conjunction with the accompanying drawings. The following embodiments are only descriptive, not restrictive, and cannot limit the protection scope of the present invention.

[0017] Design idea of ​​the present invention is:

[0018] Design and synthesize lncRNA-HZ5 siRNA, and then use liposome reagents to transfect bovine skeletal muscle satellite cells to interfere with the expression level of lncRNA-HZ5 in bovine skeletal muscle satellite cells, by changing the expression of lncRNA-HZ5 in bovine skeletal muscle satellite cells , thereby promoting the myogenic differentiation process of bovine skeletal muscle satellite cells.

[0019] Below in conjunction with accompanying drawing and specific embodiment the present invention is described in further detail:

[0020] A method for promoting bovine skeletal muscle satellite cell myogenic differentiation, comprising the following ste...

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Abstract

The invention relates to a method for promoting myogenic differentiation of bovine skeletal muscle satellite cells. According to the method, myogenic differentiation of bovine skeletal muscle satellite cells is promoted by interfering lncRNA-HZ5 expression. The specific method comprises the steps as follows: siRNA of lncRNA-HZ5 is designed and synthesized according to an lncRNA-HZ5 base sequence, siRNA is used for transfection of the bovine skeletal muscle satellite cells, the expression level of lncRNA-HZ5 is lowered, and myogenic differentiation of the bovine skeletal muscle satellite cells is promoted, wherein the genome base sequence of lncRNA-HZ5 is shown in SQ1. The myogenic differentiation process of the bovine skeletal muscle satellite cells regulated by changing lncRNA-HZ5, enlightenment is provided for long non-coding RNA research of muscle development differentiation, and a new idea and reference are provided for clinical research, diagnosis and treatment of muscle development differentiation and damage repair.

Description

technical field [0001] The invention belongs to the technical field of cell and tissue engineering in the field of biotechnology, in particular to a method for promoting myogenic differentiation of bovine skeletal muscle satellite cells. Background technique [0002] Skeletal muscle satellite cells (skeletal muscle satellite cells) are myogenic stem cells with differentiation and proliferation potential in skeletal muscle. They usually exist between the sarcolemma and basement membrane of muscle fibers in a resting state, and can be activated under certain conditions. Proliferation and myogenic differentiation to form skeletal muscle cells, muscle satellite cells play a very important role in the growth, development and regeneration of muscles in animals after birth. The in vitro myogenic differentiation process of muscle satellite cells well simulates the muscle development process in vivo, and is a good cell model for studying cell differentiation and muscle development. ...

Claims

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Application Information

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IPC IPC(8): C12N15/87C12N5/10
CPCC12N5/0659C12N15/87C12N2510/00
Inventor 丁向彬王轶敏刘新峰张蔚然李新张林林郭宏
Owner TIANJIN AGRICULTURE COLLEGE
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