In vitro culturing method of goat skeletal muscle satellite cells

Abstract

The invention belongs to the field of biotechnology, and especially relates to an in vitro culturing method of goat skeletal muscle satellite cells. The in vitro culturing method comprises following steps: (1) sampling; (2) digestion; (3) filtration; (4) primary culturing; and (5) induction culturing. According to the in vitro culturing method, goat skeletal muscle satellite cells can be quickly obtained via in vitro culturing, and are uniform in cellular morphology, and high in breeding vitality; and after differentiation induction, myotubes can be obtained via fusion of the goat skeletal muscle satellite cells. The in vitro culturing method of goat skeletal muscle satellite cells is simple in operation, is capable of reducing culturing cost greatly, possesses certain economic significance, and can be used for obtaining a large amount of cells with excellent subculturing stability. In vitro culturing of goat skeletal muscle satellite cells is capable of providing exploration on molecular mechanisms of muscle differentiation approaches and production of transgenic animals with improved muscle properties with abundant cell resources, and possesses theoretical values and application values in a certain degree.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an in vitro culture method of goat skeletal muscle satellite cells. Background technique [0002] Myoblasts in skeletal muscle exist in the form of muscle satellite cells (satellite cells, SC); experiments have shown that muscle satellite cells in skeletal muscles have the ability of self-renewal and myogenesis, osteogenesis, adipogenesis, etc. Called "muscle stem cells", this cell is a precursor cell directed to the myoblast line; the muscle regeneration process is mainly completed by muscle satellite cells; muscle satellite cells are located between the sarcolemma and basement membrane of muscle fibers When the muscle tissue is stimulated, such as when the skeletal muscle is damaged, the satellite cells will be activated, a large number of divisions, proliferation and mutual fusion to form regenerative muscle fibers; the activated satellite cells and the affected Damaged cells fus...

AI Technical Summary

Problems solved by technology

[0003] At present, there is no report on the isolation and culture of goat muscle satellite cells, and the functional study of muscle fiber satellite cells is of great significance to goat breeding. It is necessary to establish a complete set of in vitro culture methods for goat muscle fiber satellite cells