183 results about "G penicillin" patented technology

The invention relates to a genetic-engineering antibacterial peptide, as well as a preparation method and application thereof. The preparation method comprises the following steps: according to the characteristics of the amino acid sequence of Magainin and Cecropin P and LL-37 which are antibacterial peptide, a novel antibacterial peptide gene is designed; the sequence of the gene is synthesized and transformed to be expressed in pichia to form antibacterial-peptide gene transformation pichia engineering bacteria, and to be expressed in a fermentor to achieve the effects of high-density cultivation and efficient expression. Fermentation broth is separated and purified to be an antibacterial peptide product which can be applied to feed and food additives and be used as injection. The antibacterial peptide has the advantages of good antibacterial activity to most gram-negative bacteria and gram-positive bacteria, in particular better effects of inhibiting and killing ampicillin-resistant bacteria and kanamycin-resistant bacteria.

The invention discloses a magnetic polymer microsphere for enzyme immobilization and a relative preparation method, which uses hydrophilic nanometer magnetic particles as magnetic material, uses vinyl compound as functional monomer, uses composite surface activator as disperser and uses inverse suspension polymerization technique to prepare the macromolecule polymer pear carrier with narrow grain distribution and superparamagnetic and hydrophilic expoy group. The immobilization penicillin acylase prepared by the magnetic carrier has high apparent activity as 330IU/g (humidity), and the immobilization enzyme can be recovered easily to be circulated by external magnetic field, thereby improving the utilization of immobilization. The preparation method has simple process, easy operation, low production cost and support for large-scale production.

The invention discloses a biological cell freezing antifreeze, and 100ml of the cell freezing antifreeze consists of the following raw materials: 0.025 to 0.3g of kelp polysaccharide, 2.0 to 3.5g of glucose, 0.5 to 1.5g of sodium citrate, 60 to 80ml of distilled water, 10 to 20ml of albumin, 5 to 18ml of glycerol and 0.05 to 0.15 million units of penicillin. The preparation method is that: the glucose and the sodium citrate are dissolved in the distilled water to prepare a base liquid; the albumin is added, the mixture is mixed evenly, and the penicillin is added to prepare a I liquid; the glycerol is added in the I liquid to prepare a II liquid; the kelp polysaccharide is added in the II liquid for even mixing; the pH value is adjusted to 6 to 7.5, then the filtration and sterilization are carried out, and the mixture is cooled until reaching the room temperature and then arranged in a refrigerator of 2 to 5 DEG C for standby. The cell freezing antifreeze is applicable to the cryopreservation of mammalian semen, somatic cells, oocytes, early embryos, organs and tissues, in particular to the cryopreservation of the semen of various mammals.

The invention relates to the fields of cytobiology and cell culture, and particularly relates to a breast cancer organoid culture kit. A method for realizing the kit comprises the following steps: (1)preparing, sub-packaging and using a breast cancer organoid culture medium A; (2) preparing, sub-packaging and using a breast cancer organoid culture medium B; and (3) preparing, sub-packaging and using a sample preserving fluid. The breast cancer organoid culture medium A is a DMEM/F12 culture medium containing 100 U/ml of penicillin, 0.1 mg/ml of streptomycin, 1% of HEPES and 1% of Gluta Max 100 X; the organoid culture medium B is a DMEM/F12 culture medium containing EGF, an FGF growth factor, an ALK inhibitor A83-01, Noggin, an HEPES buffer solution, Y27632 and one or more of a Wnt signalpath related secretory protein family R-Spondin 1-4; and the sample preserving fluid is a DMEM/F12 culture medium containing 100 U/ml of penicillin, 0.1 mg/ml of streptomycin and 1% of L-glutamine. The three components form the breast cancer organoid culture kit.

The invention discloses a process for preparing 6-aminopenicillanic acid, which comprises the following steps: (1) filtering the penicillin fermentation liquid, acidifying, extracting and concentrating with butanol, decoloring to obtain butyl extract of penicillin, (2) subjecting butyl extract of penicillin to back extraction with alkaline solvent, obtaining aqueous solution of penicillin salts, (3) degreasing the aqueous solution of penicillin salts, (4) loading the degreased aqueous solution of penicillin salts into penicillin acylated enzyme retort for enzyme conversion, charging 6-APA seeds, cultivating quartz, crystallizing and drying.

The invention provides a penicillin G acylase mutant for synthesis and an application thereof in the preparation of amoxicillin. Penicillin G acylase of Achromobacter xylosoxidans origin is mutated by computer aided design in connection with semi-rational design of site-saturation mutagenesis technique and enzyme engineering modification of orthogenesis, thus acquiring the penicillin G acylase mutant lower in hydrolytic activity, better in synthetic activity, higher in synthesis-hydrolysis ratio (S/H), higher in acid resistance and better in stability, and amoxicillin can be catalytically synthesized more effectively and quickly. Immobilized enzyme hydrolytic activity of the mutant SPGA-4 obtained is decreased by 8.7 times, synthetic activity is increased by 5.6 times, the S/H ratio is increased by 8 times, the activity remains at 79% for 60 min under the condition of pH 2.0, amoxicillin is catalytically synthesized by a solid method at 10 DEG C or 20 DEG C, substrates 6-APA and D-HPM are directly charged in a solid form without dissolving, reaction pH need not be controlled, substrate conversion rate is higher than 99%, continuous use is available in more than 300 batches, and good operational stability is given.

The invention relates to a preparation method of a frozen pig semen diluent. The frozen semen diluent comprises four diluent components: a diluent A comprises glucose, sodium citrate, sodium chloride and penicillin; a diluent B comprises lactose, glucose, penicillin, streptomycin and fresh yolk; a diluent C comprises glucose, sodium citrate, sodium chloride, penicillin, glycerol, coconut oil monoethanol amide, lauryl sodium sulfate, triethanolamine, hydrochloric acid and distilled water; a diluent D comprises anhydrous citric acid, caffeine g, trihydroxymethyl amino methane, sodium bicarbonate, ethylenediamine tetraacetic acid disodium salt, sodium pyruvate and glucose; the preparation steps are as follows: respectively dissolving raw materials in 100ml of double distilled water to prepare four diluents, regulating the pH values to be 6.5-7.5, and refrigerating in a refrigerator. The product disclosed by the invention is used for the freezing storage of the pig semen diluent.

The invention discloses anti-oxidative cattle frozen semen diluent. The anti-oxidative cattle frozen semen diluent is composed of the following raw materials by mass: 20-26 parts of trihydroxy methyl-aminomethane, 10-15 parts of trisodium citrate, 7-15 parts of fructose, 700-800 parts of distilled water, 230-260 parts of fresh yolk, 0.125-0.375 part of sodium selenite, 2-32 parts of Vitamin E, 90-95 parts of glycerol, 1-2 parts of penicillin potassium for injection (specification of 1.6 million units/g) and 1-2 parts of streptomycin sulfate for injection (specification of 1.0 million units/g). The preparation method of the anti-oxidative cattle frozen semen diluent comprises the steps of preparing a basic buffer solution, a basic diluent and a semen diluent; then transferring the prepared semen diluent to a condition of 4 DEG C, and balancing for 5-20 min to obtain the anti-oxidative cattle frozen semen diluent. Compared with a conventional anti-oxidative cattle frozen semen diluent, the anti-oxidative cattle frozen semen diluent provided by the invention increases semen motility rate by 24.56%, increases 4 h semen motility rate by 12.39%, increases semen perforatorium integrity rate by 38.72%, increases GSH-PX activity by 260%, and reduces semen aberration rate by 5.49%.

The invention discloses preparation and an enzyme-linked immunosorbent assay method for heavy metal mercury polyclonal antibody in the technical field of heavy metal detection. The polyclonal antibody is prepared for the enzyme-linked immunosorbent assay by the following steps of: bonding the heavy metal mercury at one end of penicillin G sodium serving as a dual-functional chelating agent; coupling the other end with high molecular carrier proteins, namely bovine serum albumin and ovalbumin, to form immunogen MMPA-BSA and envelope antigen MMPA-OVA of mercury-chelating agent-protein; and finally emulsifying and immunizing the immunogen MMPA-BSA to prepare the polyclonal antibody. The prepared antibody has high specificity for the mercury ions, a cross reaction ratio with all other metals of less than 0.001 percent apart from the cross reaction ratio with cadmium of 7.9 percent and an adding recovery ratio of between 91.4 and 120 percent; and thus the antibody can be used for the assayof the heavy metal mercury in a water sample and can also be developed into rapid immunological detection technology for rapidly detecting the heavy metal mercury in other samples such as agricultural products and the like by combining certain pre-processing technology.

The invention relates to a method for preparing penicillin-G-1-(S)-oxide. In the method, penicillin G is used as a raw material, and the oxidization of the penicillin G and the crystallization of penicillin-G-1-(S)-oxide which is the oxidization product of the penicillin G are performed at the same time. A penicillin-G-1-(S)-oxide crystal product is obtained by directly adding an oxidant into penicillin G organic phase solution, reacting and crystallizing, the oxidization reaction and the crystallization are performed at the same time, and the crystal product directly precipitates in organic solution. Compared with the conventional direct oxidization process, the method has the advantages that: the operation flow is simplified considerably; the operation is simple and convenient; the labor intensity is lowered; and the production period is shortened obviously. The process of extracting the penicillin G from the organic phase back to a water phase and the process of regulating pH value with diluted acid and crystallizing are saved, so the consumption of waster resources is reduced greatly, the discharge of waste acid liquor is reduced, and the problem of environmental pollution is alleviated obviously. The high-performance liquid chromatography (HPLC) content of the product reaches over 99.0 percent, and the weight yield of products with a main particle size over 30 mu m is over 90 percent.

The invention discloses benzopyrone-phenyl-oxazolidone compounds with the following structural general formulas shown in the specification. The compounds have better inhibiting and killing effects on various germs, and some of the benzopyrone-phenyl-oxazolidone compounds have higher bacteriostatic activity than positive control penicillihe G, kalamycin and ketoconazole, so that the benzopyrone-phenyl-oxazolidone compounds can be used for preparing anti-infective drugs. The invention also discloses preparation methods of the benzopyrone-phenyl-oxazolidone compounds.

The invention discloses a heterogeneous biocatalyst taking alpha-amino-acid ester hydrolase as basis, a preparation method thereof and application in synthesizing amino beta-lactams. The activity of catalyst synthetase is 2,200 U/g by dry weight, and the content of protein is 0.95 g/g by dry weight; and xanthomonas rubrilineans cell biomass is processed in an organic solvent under a pH gradient through low temperature effect to extract enzyme, and enzyme aggregates are deposited and cross-linked to obtain the hydrolase. The alpha-amino-acid ester hydrolase as a catalyst can be synthesized into amino penicillin and amino cephalosporin drugs through acylating a beta-lactam compound by D-phenylglycine methyl ester derivatives in water or in a mixed medium of water and an organic solvent.

The invention relates to the technical field of biosensors, especially relates to a fluorescent biosensor based on hybrid chain reaction amplification, and solves the problems in the prior art that the method for detecting ampicillin is low in specificity and sensitivity, and the cost is high. According to a biosensor for detecting the ampicillin based on an aptamer, a cyclic amplification effectis realized by the cooperation of Nb.BbcCI and a chain hybrid chain reaction, and Thioflavine-T and G-quadruplex are combined to generate fluorescence and a homogeneous reaction mixed solution. The preparation method comprises the following steps of preparing gold nanoparticles; modifying Walker and Track to the surfaces of the gold nanoparticles; mixing a labeled nanogold solution with the homogeneous reaction solution; performing hyperbranched hybrid chain reaction and fluorescent detection; carrying out high specificity detection on the target ampicillin by using the aptamer by utilizing the specific recognition of the aptamer; and using hyperbranched hybrid chain reaction amplification to achieve signal amplification.

A process for the use of low concentration levels of Penicillin G Procaine to eliminate or control the growth of unwanted or undesirable bacteria (contaminating bacteria) in the fermentation production of alcohol without inhibition of the growth or replication of the yeast.

The invention provides a mycoplasma anatis culture medium. The mycoplasma anatis culture medium is composed of a liquid culture medium and a solid culture medium. The liquid culture medium is prepared from deionized water, a 10% thallium acetate solution, PPLO broth, glucose, peptone, tryptone, 1% phenol red, a CMRL-1066 culture medium containing L-glutamine, inactivated horse serum, yeast leachate, penicillin, L-cysteine hydrochloride and nicotinamide adenine dinucleotide. Besides the situation that agar powder is added and phenol red is removed, other components of the solid culture medium are the same as those of the liquid culture medium. The culture medium is used for separating and purifying mycoplasma anatis, wherein a bacterial colony is separated from the solid culture medium, the separated bacterial colony is placed in the liquid culture medium to grow, then filtering, dilution and plate coating of liquid culture substances and purification of picked single colony multiplication are conducted, and the steps are repeated till purified mycoplasma anatis is obtained. The culture medium can promote growth of mycoplasma anatis, and the success rate of separation and purification of mycoplasma anatis is high.

The invention provides a preparation method of methoxybenzyl, which uses penicillin and saturated salt water containing hydric sulphate. The invention uses penicillin as raw material to obtain penicillin sulfoxide via oxidization, to obtain penicillin sulfoxide ester via esterification, to be reacted with benzene sulfinate, to open ring and generate nitrogen heterocyclic butanone sulfinic acid intermediate, and electrolyzes the saturated salt water containing hydric sulphate, to utilize generated chlorizating agent to chlorizate the intermediate, at last ammonolyzes and closes ring to generate GCLE. The invention has the advantages of high product yield, high purity, low cost, and high quality, which uses electrolysis close-ring to process chlorization in production to assure safe reaction.

The invention relates to a method for synthesizing cefprozil through a green enzymatic method. The method includes following steps: S1, adding parent nucleus 7-APRA or hydrochloride thereof into a buffer solution, and adding D-para hydroxybenzene glycinate derivative and/or D-para hydroxybenzene glycine amide under the condition that pH is 5-8; S2, adding cefprozil synthetase into the S1, reacting at temperature of 15-30 DEG C and pH of 6.5-7.8 for 1-3h, separating out separation liquid after reaction finishes, and immobilizing cefprozil synthetase to obtain a cefprozil crude product; S3, subjecting the crude product obtained in the S2 to acidolysis dissolved clarification, filtering and re-crystallizing to obtain cefprozil. Raw materials are cheap and easy to obtain, reaction is simple, hydrolysis activity of penicillin acylase can be effectively inhibited, and production cost is low; compared with conventional chemical synthesis methods, the method is simple and convenient to operate and low in cost, synthesis period is shortened, production efficiency is improved, total yield is high, controllability is high, and needs on industrial production are met.

The present invention relates to 7beta-alkylamido-3-(1-methyl-1-pyrrolidyl onium) methyl-3-cephalo olefine-4-carboxylate and its preparation process and the synthesizing process of cephalo pyroxime hydrochloride with the said intermediate. The present invention has mild reaction condition, no need of expensive reagent and benzyl penicillin acylase, low cost, high product purity, and simple and practical reaction path.

The invention discloses a culture medium for hepatoma organ cell sphere culture. The culture medium is composed of a basic culture medium, 10-25 ng/mL of an epidermal growth factor, 10-25 ng/mL of a fibroblast growth factor, 0.5-2 ng/mL of a cell growth factor, 1-3 ng/mL of an insulin growth factor, 1 mmol/L of sodium pyruvate, 2 mmol/L of glutamine, 100 U/mL of penicillin, 100 [mu]g/mL of streptomycin and 10% by volume of serum. Aiming at the culture growth characteristics of liver cancer tissue-derived cells, the culture medium provided by the invention is simple and reasonable in components, rich in nutrition and moderate in formula price; and hepatoma organ cell spheres cultured by the culture medium can effectively and stably grow for a long time, are closely gathered, have smooth andround surfaces and clear boundaries, is free of disintegration phenomenon, and can be significantly improved in cell activity, specific functions and metabolic functions.

The invention discloses a novel method for preparing penicillin G sulfoxide, which comprises the following steps: a, selecting a 60000-120000 mu/g penicillin G fermentation solution, and dropwisely adding peroxyacetic acid to obtain a solution I; b, filtering the solution I to obtain a filtrate, and concentrating the filtrate through a nanofiltration membrane of which the molecular weight cut-off is 200-800Da, thus obtaining a concentrated solution; c, regulating the pH value of the concentrated solution with sulfuric acid, and filtering to obtain a penicillin G sulfoxide crude product; and d, dissolving the penicillin G sulfoxide crude product in a methanol water solution, recrystallizing, then cooling to 0-10 DEG C, filtering, washing the obtained crystals with methanol, swabbing off, and performing microwave drying on the obtained product to obtain the penicillin G sulfoxide. According to the invention, the method is simple, the process period is short, and extraction treatment can be performed without using a large amount of organic solvent. Thus, the method disclosed by the invention is low in cost and environment-friendly.

The present invention relates to a mutant penicillin expandase which comprises an amino acid substitution at one or more residue positions corresponding to those of a wild-type expandase selected from the group consisting of threonine at position 42, isoleucine at position 50, histidine at position 57, threonine at position 67, valine at position 133, threonine at position 143, proline at position 145, glycine at position 148, phenyl alanine at position 152, proline at position 196, alanine at position 240, cysteine at position 281, Serine at position 309, provided that the amino acid substitution at the residue position of cysteine at position 281 is not tyrosine.

The invention relates to a culture medium for separating thraustochytrium limacinum and a purification method thereof. The culture medium for separating thraustochytrium limacinum is prepared from 1-5g of Glucose, 0.5-2 g of Peptone, 0.5-2 g of Yeaast exact power, 0.5-1 g of sodium glutamate, 0.5-1 g of corn steep liquor, 15-20g of Aar, 20-30g of sea salt, 0.5-1 g of penicillin G, 0.5-1.5 g of Streptomycin, 50-100 mg of rifampicin, 5-20 mg of Nystatin, and 1L of H2O, then steps of strain isolation, morphological observation, 18S rRNA sequencing are carried out; according to the method, thraustochytrium limacinum can be directly separated from rotten sea grass in marine water and wetland, the culture medium is simple in component, low in cost and convenient to operate, and the method can beused as a separation culture method of thraustochytrium limacinum for laboratories.

The invention discloses a preparation method of a penicillin G sodium salt surface molecular imprinting polymer. The preparation method comprises dissolving penicillin G sodium salt in a solvent which is a mixture of dimethyl sulfoxide and methanol according to a volume ratio of 4: 1, adding a functional monomer into the solution, carrying out stirring, adding a cross-linking agent and an initiator into the mixed solution, carrying out nitrogen deoxygenation, carrying out a heating reaction process, carrying out washing to remove penicillin G sodium salt as a template molecule after the reaction and carrying out drying to obtain the penicillin G sodium salt surface molecular imprinting polymer. The preparation method has simple processes, good operationality and a low preparation cost. The penicillin G sodium salt surface molecular imprinting polymer has a fast penicillin sodium salt molecule bonding rate, good specificity, high bonding capacity and short adsorption balance time. The penicillin G sodium salt surface molecular imprinting polymer has high desired sample selectivity, good specificity and recovery and recycle of a template molecule and can be used for detecting a certain concentration of penicillin G sodium salt residues in milk.

The invention relates to a preparation method of a diluent for storing sheep semen at a normal temperature. The preparation method comprises the following steps: 1, dissolving 30.2 g to 31.2 g of Tris, 15.9 g to 16.9 g of citric acid, 19.5 g to 20.5 g of fructose, 0.3 g to 0.4 g of penicillin sodium and 0.6 g to 0.7 g of streptomycin sulfate into 1000mL of sterilized ultrapure water to obtain a basic diluent, 2, when 95 mL of the basic diluent is taken, adding 5 mL of yolk into 95 mL of the basic diluent so that a first diluent is obtained, when 85mL of the basic diluent is taken, adding 15mLof yolk into the 85mL of the basic diluent to obtain a second diluent, when 80 mL of the basic diluent is taken, adding 20 mL of yolk into 80 mL of the basic diluent so that a third diluent is obtained, putting the first diluent, the second diluent and the third diluent into a refrigerator to be fully dissolved, then centrifuging for 20 minutes at the speed of 10,000 r/min and the temperature of 4DEG C, and taking out a supernatant after centrifuging, so as to obtain a yolk diluent with the concentration of 5%, a yolk diluent with the concentration of 15% and a yolk diluent with the concentration of 20%. Through the diluent provided by the invention, the semen preservation effect is improved.

The invention discloses a reverse osmosis membrane alkaline cleaning agent. The alkaline cleaning agent comprises the following components in parts by weight: 18-22 parts of sodium hydroxide, 32-35 parts of sodium tripolyphosphate, 16-18 parts of sodium carbonate, 14-16 parts of ethylenediaminetetraacetic acid tetrasodium salt, 7-8 parts of sodium bisulfite, 2-3 parts of sodium dodecyl benzene sulfonate, 2-3 parts of sodium dodecyl sulfonate and 0.5-1.5 parts of alkaline protease. The reverse osmosis membrane alkaline cleaning agent provided by the invention can be used for efficiently cleaning out pollutants on a reverse osmosis membrane element which is used for treating penicillin production waste liquid. The alkaline cleaning agent is low in cost, is convenient to transport and store has low cost, and has a shelf life of 12 months or above.