72 results about "Cephalopod" patented technology

The invention provides a method for managing spartina alterniflora. The method comprises the following steps of cutting off the spartina alterniflora; turning over intertidal zones; and planting embryonal axes of kandelia candel. Management on soil, with the depth of 0-20cm, for the spartina alterniflora is replaced by cutting off the spartina alterniflora, turning over the intertidal zones and planting the kandelia candel; the organic matter content and the N (nitrogen) content of the soil are obviously increased; the salinity of the soil is obviously improved by the high-density kandelia candel; pH (potential of hydrogen) of the soil is reduced, and is continuously reduced along with increasing of the density of the kandelia candel; influence on the P (phosphorus) content of the soil is quite low; the organic matter content of the soil with the depth of 20-40cm is increased, and is positively correlated with the density of the kandelia candel; the N content, the P content and the pH of the soil are reduced; and the pH is continuously reduced along with increasing of the density of the kandelia candel. After the kandelia candel is planted for one year, the density and the biomass of large benthonic animals are increased; and the density and the biomass of polychaetes animals, crustacean animals and other animals and the biomass of mollusk are improved. The method can be effectively used for managing the spartina alterniflora.

The invention relates to a method for grinding and protecting cephalopod otolith. The method comprises the steps of extraction, soaking, placing, resin embedding, cutting, fixing on a glass slide, abrading, polishing, preservation and the like. The method can solve the problems that the cephalopod otolith is small and not easily ground, the otolith is easily broken in the grinding process and thetextures are easy to fade after polishing; and the otolith textures can be kept for long time.

The invention pertains to biological resources and environmental technology, in particular to a marine caphalopoda animal I collagen protein product and the preparation method thereof. The invention discloses the marine caphalopoda animal collagen protein, the collagen protein is extracted from the skin of marine caphalopoda animals, the collagen protein is white or white with the light brown and is the I type collagen protein, the molecular type is (Alpha 1)2Alpha2 type, the molecular weight of the main peptide chain is 300 to 330KD, the intrinsic viscosity is 12 to 18dL/g, the denatured temperature is 15 to 30 DEG C, and every 1000 amino acid residues contain 280 to 330 parts of glycine, 80 to 90 parts of proline and 60 to 70 parts of hydroxyproline respectively. The preparation method takes squid skin which is one of the processing waste of the seafood as raw material for preparing the I type collagen protein; an edible biochemical reagent, mild preparation conditions and simple physical method are adopted for carrying out the extraction of the collagen protein in the squid skin; the obtained collagen protein product dose not have the residue problem of the toxic and harmful reagents, which has very high safety; therefore, in addition to the general usages of the collagen protein, the marine caphalopoda animal I collagen protein product can also be directly applied in the industries of general foods, special foods, functional foods and biomedicine; the usage of the method for preparing the collagen protein is environmental-friendly, which does not discharge toxic and harmful substances to the environment and can avoid the new pollution to the environment of certain preparation methods.

The invention provides a method of identifying day age of cephalopods through beaks and includes a method of grinding a beak longitudinal section and counting growth striation. The method mainly includes the steps of: extraction, cleaning, soaking, cutting, arrangement, resin embedding, cutting, immobilization on a glass slide, grinding and polishing, picture forming, and growth striation counting. In the method, the beak longitudinal section is ground respectively from the left and right sides to a core surface towards a central line. A prepared section is photographed by CCD to ensure that the growth striation can be read at any time in future. The growth striation is counted from the rear end to the front end of the beak in a direction being vertical to the growth striation all the time, thereby ensuring counting accuracy. The method solves the problems that the beak is difficult to grind and is liable to break during grinding, and the growth striation is difficult to observe and count.

The invention provides a method for forecasting the fishing situation of Symplectoteuthis australis in the South China Sea, which comprises the following steps: different points are selected in the sea area where the fishery situation prediction of Symplectoteuthis australis needs to be carried out, and environmental factors affecting the habitat quality of Symplectoteuthis australis at corresponding positions are obtained; the environment factors affecting the habitat quality of Symplectoteuthis australis are analyzed; according to the model of different seasons and different environmental factors and adaptability index, the adaptability index of the selected point position is obtained; according to the model of habitat adaptability index and seasonal adaptability index, the habitat adaptability index of selected sites is obtained; the habitat adaptability index distribution map of Symplectoteuthis ulna is obtained by image processing of habitat adaptability index corresponding to theselected points in the fishery forecasting sea area, and the range of Symplectoteuthis ulna fishery could be predicted. The method of the invention can make short-term prediction of the fishery situation of the South China Sea kite squid and reflect the habitat change of the cephalopod resources in a short time, and the accuracy rates of forecasting the fishery situation of the South China Sea kite squid in different seasons are 83%, 86%, 88% and 90%, respectively.

The present invention relates to pure aquatic ham sausage or western style ham sausage containing fish, which made from pure aquatic meat. The ham sausage or western style ham sausage according to the invention is produced by using the minced cephalopod aquatic meat as a binding agent, mixing with fish as a main material, adding flavorings to the mixture, and processing by conventional methods, wherein the cephalopod aquatic meat comprise 8-95% of the total weight. The present invention make use of high binding ability of the cephalopod aquatic to overcome the defects of the fish which difficult to processed due to its high moisture content and short fibres, and use cephalopod aquatic meat instead of animal and bird meat in the ham sausage or western style ham sausage using chopped fish as raw material, making it become possible that the pure aquatic ham sausage or western style ham sausage can be made. The present invention provide a new way to process aquatic, keep the original flavor and nutrition of aquatic animal to maximum extent and the ham sausage or western style ham sausage obtained is more delicious and better in taste comparing the existing aquatic ham sausage or western style ham sausage prepared with animal and bird meat.

The disclosed invention relates to novel materials and associated methods for conducting protons, such materials comprising cephalopod proton-conducting proteins such as reflectins. The protonic conductivity of such cephalopod proton-conducting proteins may be modulated by the application of an electric field. The invention further encompasses protonic transistors comprising a cephalopod proton-conducting protein channel. The transistors and related devices of the invention are amenable to use in biological systems for the sensing or manipulation of protonic flows within the biological system.

The invention provides a method for studying growth of cephalopods through classification of beak pigmentation. The method comprises the following steps: grade classification is performed on beak pigmentation according to different positions and different degrees of beak pigmentation of Ommastrephidae and in combination of beak individual size and Ommastrephidae individual size; on the basis of the AIC (Akaike information criterion), relationships of pigmentation grades with biology and beak parameters are established, growth characteristics of the Ommastrephidae are studied, and growth stages of Ommastrephidae individuals are determined according to beak pigmentation grades. According to the method, morphological characteristics of cephalopod beaks are summarized, and laws of pigmentation in different periods and different positions are found out; the beak pigmentation grades of the cephalopods are classified rapidly and concisely; the relationships of the pigmentation grades with biology and the beak parameters are established for the first time, deep study are performed better on growth and ingestion conditions of the cephalopods, the pigmentation grades are in one-to-one correspondence with individual growth stages directly, and the growth stages of the individuals can be judged directly through the beak pigmentation grades.

The invention relates to the field of aquatic science, in particular to a method for measuring the age and individual growth of sthenoteuthis oualaniensis on the basis of the external form of otolith.According to the method, the age and individual growth of sthenoeuthis oualaniensis are measured by utilizing four appearance feature parameters including the total statolith length, maximum width, lateral dome length and wing length of the otolith of sthenoeuthis oualaniensis. According to the method, the research on the correlation between the age and individual growth of sthenoeuthis oualaniensis and the external form of the otolith is carried out, and a necessary basis is provided for utilizing the external form of the otolith to carry out basic biologic and ecologic research on cephalopods and resource quantity evaluation.

The invention aims to provide a pretreatment reagent for living body transportation of parents of cephalopods. The pretreatment reagent can improve the effect of living body transportation of parents of cephalopods and has the advantages of simple operation method, low cost, good anesthetic effect, low action concentration, controllable resuscitation time, stable performance and low damage to fish body. According to the invention, aniline hydrochloride acetyl propyl aminobenzoate, a derivative of anthranilic acid, is used as main active component of anesthetic used in pretreatment during the process of living body transportation, which has good anesthetic effect on cephalopods, low action concentration, controllable resuscitation time and stable performance. In addition, after combined use with CaCl2, the anesthetic effect of aniline hydrochloride acetyl propyl aminobenzoate at low-temperature is basically not reduced.

The invention provides an artificial maturation method of cephalopods, comprising: soaking a cephalopod in sea water containing 2.5-3.8% of polyurethane for anesthesia, drilling at eye socket sinuses of the cephalopod, pushing in GnRH slow-release embedded blocks with tweezers, and allowing the blocks to float freely in the sinuses. The artificial maturation method of cephalopods has the advantages that the GnRH slow-release embedded blocks are implanted in the eye socket sinuses under eyes so that cephalopods are maturated and induced for spawning, and such method allows cephalopods to be maturated quickly with good safety and zero harm; the prepared GnRH slow-release embedded blocks take a long time to release at uniform speed, are biologically degradable, can release completely and may cause zero environmental pollution or energy waste; the operation steps are simple, the results are significant, and artificial breeding of marine cephalopods is never dependent on their natural breeding and egg spawning.

The invention relates to universal primers capable of highly efficiently and quickly amplifying cephalopod mitochondrial COI genes of various cephalopods, and a design and amplification method thereof. By designing the universal primers of the cephalopod mitochondrial COI genes, various cephalopod COI genes can be amplified at a time in batches, specific primers are not needed to be designed for acertain single species, the time cost is saved, the work efficiency is greatly improved, and the method has high-efficiency and fast characteristics and provides important molecular means and data accumulation for species identification, systematic evolution, germplasm resource conservation and sustainable development and utilization of the cephalopods.

The invention discloses a novel ink melanin extraction method, which comprises the following steps: (1) microwave removal of polysaccharides; (2) ultrasonic removal of fats; (3) enzymatic hydrolysis of proteins: adding a disodium hydrogen phosphate-potassium dihydrogen phosphate buffer solution with a pH value of 7.5 to 8.5 into precipitates obtained in step (2) according to a material-solution ratio of 2.5 to 4.5g/ml, regulating the temperature to 45 to 55 DEG C after uniform stirring, adding 3.2 to 4.4 percent of plant proteinase, performing reaction for 1 to 1.5h, regulating the pH value of the buffer solution to 4.9 to 6.1, removing supernatant after precipitates are separated out, and rinsing with sterile water and drying the precipitates, wherein the plant proteinase is gingko leaf proteinase or myrica rubra leaf proteinase. Compared with the prior art, the method has the beneficial effects that cephalopod processing residues, i.e. ink bags, are adopted as extraction raw materials, so that the cost is low, and environmental pollution is reduced; a crude plant protein enzyme solution with high enzyme activity is adopted to remove the proteins, so that the time consumption is low, the efficiency is high, and an obtained ink melanin is high in purity and solubility.

The present disclosure relates to molluscicide compositions, (e.g., saponin and caffeine from a different source) that act synergistically as molluscicides. Also provided are methods comprising the disclosed molluscicide compositions to control, treat, prevent, and combinations thereof mollusk infestation.

The invention discloses a Mauritania cephalopod fishery prediction method based on habitat indexes. According to the method, SST, SSHA and sea depth obtained by satellite remote-sensing are combined to analyze Mauritania cephalopod fishery distribution and a habitat index model thereof under different environment weights according to production statistics collected and acquired at Mauritania fishery work from 2010 to 2015, and a basis is provided for carrying out Mauritania cephalopod fishery forecasting. Relationships of the Mauritania cephalopod fishery distribution and marine environments are very close, and suitable environment ranges of work fisheries between January and April and July and December are also different to a certain extent. Optimum work fishery SST, SSHA, sea depth are 16-22 DEG C, -35-25cm and -5-5cm and 15-25m and 45-75m respectively. According to the model, degrees of impacts formed by different environment factors on the cephalopod fisheries are not the same, theSST is greatest in impacts, the SSHA is the second, and the sea depth is the last.

The invention relates to the field of aquatic products science, in particular to a method for research of feeding ecology of cephalopods in different life history stages according to eye crystalline lens. The method includes eye crystalline lens section stripping and stable carbon and nitrogen isotope determination in the different life history stages and mainly includes steps of extracting, cleaning, storing, crystalline lens layered stripping, drying, grinding, embedding, stable carbon and nitrogen isotope determination, feeding ecology analysis and the like. Different sections, representing different time periods, of eye near-end crystalline lens are subjected to layered stripping sampling from the periphery to the core, and an isotope ratio mass spectrometer is adopted for stable carbon and nitrogen isotope determination and feeding ecology analysis of eye crystalline lens different in section. By the method, the problem of difficulty in acquisition of feeding ecology information of the cephalopods in different life history stages is solved.

The disclosure provides a method of generating a sterile sex-determined fish, crustacean, or mollusk. The method comprises breeding (i) a fertile homozygous mutated female fish, crustacean, or mollusk having at least a first mutation and a second mutation with (ii) a fertile homozygous mutated male fish, crustacean, or mollusk having at least the first mutation and the second mutation to produce the sterile sex-determined fish, crustacean, or mollusk. The first mutation disrupts one or more genes that specify sexual differentiation, the second mutation disrupts one or more genes that specify gamete function, and the fertility of the fertile homozygous female fish, crustacean, or mollusk and the fertile homozygous mutated male fish, crustacean, or mollusk has been rescued. The disclosure also provides methods of making broodstock for use in producing sterile sex-determined fish, crustacean, or mollusks, as well as the broodstock itself.

The invention discloses a high-density hatch bucket for cephalopods. The hatch bucket includes a circular breeding tank. A whole tank inflation device which can move in the breeding tank and perform inflation on a water body in the breeding tank for oxygenation is arranged in the breeding tank. The whole tank inflation device is provided with a horizontally arranged micro-porous tube. An annular inflation ring frame is composed of the micro-porous tube, vertical tubes and a floating frame tube, wherein the vertical tubes are connected to the two ends of the micro-porous tube, and the floating frame tube is located on the top. The inflation ring frame is connected with a floater. A winding prevention hose which can supply air to the micro-porous tube is connected to the floating frame tube and provided with a winding prevention device. A conical flow guide protrusion is arranged in the center of the bottom of the circular breeding tank. To-be-hatched egg granules of the cephalopods form intermittent circulation flow under the action of the conical flow guide protrusion in the center; compared with the prior art that intermittent circulation flow is formed by opening or closing an inflation pump, the high-density hatch bucket has higher reliability, and the service life of an inflator can be prolonged. The hatch bucket has the advantages of being good in oxygenation effect, low in usage cost, large in oxygenation section, high in effective oxygenation water body ratio, efficient and capable of saving energy and protecting an oxygenation motor.

The invention aims to provide a preparation method for cephalopod transparent specimens. The method pays more attention to corrosion transparent treatment of surface muscles to highlight the internalstructure of the shellfish specimens, dyeing is performed by using different dyeing agents, so that the specimens are more ornamental; therefore, the problem of poor preparation effects of cephalopodtransparent specimens in the prior art is solved. Compared with a traditional method using alcohol to preserve specimens, the method provided by the invention avoids problems such as specimen dehydration shrinkage, protein denaturation and specimen rigidity; compared with a method using formalin to preserve specimens, the method using glycerol to preserve the specimens is non-toxic and safer; anddue to the effects of dyeing and corrosion transparent treatment, the specimens are more beautiful, and the prepared specimens can be used not only for scientific observation but also for art craft display.

The invention relates to the research field of cephalopod mitochondrial genome, and especially relates to a portunidae mitochondrial COI gene universal primer, and design and amplification methods thereof. The universal forward primer of mitochondrial COI gene of Portunidae is SEQ ID NO. 1: 5 '- TCNACAAAYCATAAAGAYATYGG-3 ', and that reverse prim is SEQ ID NO. 2: 5'-TANACYTCWGGRTGHCCRAARAAYCA- 3'.The present invention solves the problem that the prior art does not utilize the DNA barcode technology to identify the family Portunidae, and has the following advantages: 1) the universal primer ofmitochondrial COI gene of the family Portunidae provided by the present invention can amplify a plurality of families of Portunidae in batch at one time with high efficiency, and can provide great convenience for the species identification of the family Portunidae; 2) design universal primers in two conservative region of mitochondrial COI gene of family Portunidae to amplify an intermediate mutation sequence, that amplification length is about 600-700 bp, sufficient information sites are used for identification and differentiation of physical evidence, and a sequencing reaction can be complete, the operation is simple, and the cost is saved.

The purpose of the present invention is to provide a cephalopod breeding basket or a cephalopod escape preventing sheet, which can keep cephalopods in a viable state and be used for cultivating, domesticating, displaying, transporting, and storing cephalopods, and prevents cephalopods from escaping. Provided is a cephalopod breeding basket (10) that can keep cephalopods in a viable state and is configured such that a net-shaped sheet having a mesh number of at least four is used in at least a portion (a side surface section (1)) thereof. Also provided is a cephalopod escape preventing sheet that can keep cephalopods in a viable state and is configured such that a net-shaped sheet having a mesh number of at least four is used in at least a portion thereof.

The invention discloses a precise-control breeding system with an annular pipeline for cuttlefish individuals, and belongs to the technical field of artificial breeding of cuttlefishes. The breeding system comprises the annular pipeline and a water quality precise-control mechanism. The upper surface of the annular pipeline is connected with a feeding opening and a water inlet pipeline, and the lower surface of the annular pipeline is connected with a collection opening and a water outlet pipe. The water quality precise-control mechanism is connected with a detection and adjustment mechanism,a water supply pipeline and a water pumping pipeline. The water supply pipeline is connected with the water inlet pipeline through a water inlet hose, and the water pumping pipeline is connected withthe water outlet pipeline through a water outlet hose. According to the breeding system, the annular pipeline is used in combination with the water quality precise-control mechanism to provide an annular water body in which the bred cephalopods such as the cuttlefishes and the like can spray at any possible speed to swim backwards without causing tail collision or friction, so that the survival rate of the bred cuttlefishes is high, the appearance of the cuttlefishes when the cuttlefishes are put into the market is attractive, and the breeding value of the cephalopods such as the cuttlefishesis increased.