Antibody for detecting infectious spleen and kidney necrosis virus and its preparation and application

A spleen and kidney necrosis virus, infectious technology, applied in the field of biological immunity, can solve the problems of limited promotion and use, high false positive rate, and achieve the effect of simple operation and good specificity
CN104558173BActive Publication Date: 2017-11-10GUANGDONG HAID ANIMAL HUSBANDRY & VETERINARY RES INST
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
GUANGDONG HAID ANIMAL HUSBANDRY & VETERINARY RES INST
Publication Date
2017-11-10

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Abstract

The invention discloses an antibody for detecting infectious spleen-kidney necrosis virus and its preparation and application; the antibody for detecting infectious spleen-kidney necrosis virus is a purified rabbit anti-ISKNV-23P8 polyclonal antibody, which contains the sequence number as SEQ An amino acid residue of ID NO: 1 is an epitope antigen, and a polyclonal antibody that specifically recognizes ISKNV‑23P8 is obtained. The antibody is used to detect infectious spleen-kidney necrosis virus and is specific. The infectious spleen and kidney necrosis virus immunofluorescence detection kit and the non-diagnostic immunofluorescence rapid detection method of infectious spleen and kidney necrosis virus infection prints of the present invention have the characteristics of good specificity, simple operation, rapidity and sensitivity, and are relatively The PCR method has obvious advantages, and all operations can be completed within 1.5 hours, and can be used for the detection of ISKNV infection in the breeding process of various susceptible fish.
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Description

technical field

[0001] The invention relates to the technical field of biological immunity, in particular to an antibody for detecting infectious spleen and kidney necrosis virus and its preparation and application. Background technique

[0002] Infectious Spleen and Kidney Necrosis Virus (ISKNV) is a representative species of the genus Megalocytivirus in the family Iridoviridae, and is a type of cytoplasmic large double-stranded virus with an icosahedron. DNA virus, the genome consists of 111,362 bases, including 125 predicted open reading frames ORF (open reading frames). ISKNV can infect nearly 60 species of freshwater and marine fish, and is a huge threat to the aquaculture industry of mandarin fish, California perch and American redfish, and mandarin fish is its main infection reservoir.

[0003] Siniperca chuatsi (Siniperca chuatsi) is a fish of the genus Siniperca in the family Perciformes, commonly known as mandarin fish, crucian carp, mandarin fish, seasonal flower...

Claims

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