Fluorescent quantitative PCR method for detecting infectious spleen and kidney necrosis virus of mandarin fish and corresponding kit

A spleen and kidney necrosis virus, fluorescence quantitative technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of time-consuming, complicated operation, and inability to meet fast, simple and sensitive detection, etc., and achieve high sensitivity High performance, wide application range and stable detection results
CN111485035APending Publication Date: 2020-08-04广东美格基因科技有限公司
2 Cites 2 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
广东美格基因科技有限公司
Publication Date
2020-08-04

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a fluorescent quantitative PCR method for detecting infectious spleen and kidney necrosis virus of mandarin fish and a corresponding kit. Specific gene detection is ingeniouslyapplied to distinguish the infectious spleen and kidney necrosis virus of the mandarin fish from strains or viruses of other species, and accurate bacterial genus information is obtained through comprehensive determination. Compared with an existing mainstream detection kit, the kit for detecting the infectious spleen and kidney necrosis virus of the mandarin fish has the advantages of being highin sensitivity, rapid, convenient to use, good in specificity, rigorous and accurate in judgment and the like, and has good application prospects and market value.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the technical field of molecular detection, and in particular relates to a method for fluorescent quantitative PCR detection of mandarin fish infectious spleen-kidney necrosis virus through a specific gene and a corresponding detection kit. Background technique

[0002] Infectious spleen and kidney necrosis virus (ISKNV) belongs to the cytomegalovirus genus in the family Iridoviridae. The mature virus particle is icosahedral with a diameter of 100nm-150nm. The genome is double-stranded DNA. The mature virus Particles generally consist of an envelope, an electron-dense region, and a core. It invades mandarin fish through endocytosis and attaches to spleen tissue cells, resulting in the appearance of a large number of basophilic, homogenized cytoplasmic swollen cells with a diameter of about 15 μm to 20 μm.

[0003] Mandarin fish (Siniperca chuatsi) is one of the high-quality freshwater fish species in my country with tender mea...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More