The invention discloses a culture medium for stem cell culture. The culture medium comprises a bottom protecting cover, wherein a culture medium body is arranged in the cavity of the bottom protectingcover, a top protecting cover is meshed with the top of the bottom protecting cover, buckling grooves which are in interaction for use are formed in two sides of the top of the bottom protecting cover and the bottom of the top protecting cover, and the bottom protecting cover and the top protecting cover are made from acrylic materials; the culture medium body comprises 15% of FBS, 116.6mg/L anhydrous calcium chloride, 59.05mg/L L-leucine, 0.042mg/L linoleic acid, 0.0013mg/L anhydrous copper sulfate, 91.25mg/L L-lysine hydrochloride, 0.105mg/L lipoic acid, 0.05Lmg/L iron(III) nitrate nonahydrate, 17.24mg/L L-methionine, 8.1mg/L phenol red, 0.417mg/L of ferrous sulfate heptahydrate, 35.48mg/L L-phenylalanine, and 0.081mg/L 1,4-diaminobutane dihydrochloride. The preparation method is simple, few material instruments are used, stem cells can be massively prepared for culture, the amplification speed of the stem cells is high, the stem cell differentiation capacity is high, and the stem cells can be differentiated into multiple functional cells to achieve high scientific research and medical application value.