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Fluorescent LAMP primer used for detecting vesicular stomatitis virus and detecting method

A technology for vesicular stomatitis and a detection method, applied in the field of fluorescent LAMP primers and detection, can solve the problems of easy occurrence of false positives, low sensitivity, long detection period, etc., and achieve the best amplification effect, fast amplification speed, Easy to use effect

Inactive Publication Date: 2017-04-26
珠海出入境检验检疫局检验检疫技术中心
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a fluorescent LAMP primer and detection method for detecting vesicular stomatitis virus, aiming to solve the problem of long detection cycle, prone to false positives and low specificity in existing vesicular stomatitis virus detection , the sensitivity is not high, and the steps are cumbersome

Method used

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  • Fluorescent LAMP primer used for detecting vesicular stomatitis virus and detecting method
  • Fluorescent LAMP primer used for detecting vesicular stomatitis virus and detecting method
  • Fluorescent LAMP primer used for detecting vesicular stomatitis virus and detecting method

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Embodiment Construction

[0021] In order to more clearly explain the purpose, technical solutions and advantages of the present invention, the present invention will be described in detail below in conjunction with examples. It should be understood that the embodiments described here are only used to explain the present invention, not to limit the present invention.

[0022] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0023] The fluorescent LAMP primers used to detect vesicular stomatitis virus provided by the embodiments of the present invention are:

[0024] SEQ ID NO: 1

[0025] FIP TACCGCTGCAACTATCTTGGTGGCCTCGTTGAAGATGGATT

[0026] SEQ ID NO: 2

[0027] BIP ATGAGCGTGCACCAATCAGATGCTAATGCTGCACAGTCT

[0028] SEQ ID NO: 3

[0029] F3TGGATGGTTTAGAGAATCAGTG

[0030] SEQ ID NO: 4

[0031] B3TCCACTGACCTTGCTTAGA

[0032] SEQ ID NO: 5

[0033] LF CGGATCATTCTCCCATATGTCA

[0034] SEQ ID NO: 6

[0035] LB CGGAA...

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Abstract

The invention discloses a fluorescent LAMP primer used for detecting a vesicular stomatitis virus and a detecting method. A sequence is shown in the specifications. According to the detecting method, the final concentration of inner primers FIP and BIP is 1.6umol / L, the final concentration of outer primers of F3 and B3 is 0.2umol / L, and the final concentration of annular primers of LF and Lb is 0.8umol / L; the reaction temperature is 63 DEG C. According to the fluorescent LAMP primer used for detecting the vesicular stomatitis virus and the detecting method, fluorochrome is introduced on the basis of an original LAMP technology to establish a fluorescent LAMP detecting technology, due to the fact that an instrument is used to conduct fluorescent collecting, sensitivity of detecting is further improved, detecting is more objective, the operation is simpler and more convenient, the detecting method is easy to be standardized, and the fluorescent LAMP primer used for detecting the vesicular stomatitis virus and the detecting method are easy to be used and popularized.

Description

technical field [0001] The invention belongs to the technical field of vesicular stomatitis virus detection, in particular to a fluorescent LAMP primer and a detection method for detecting vesicular stomatitis virus. Background technique [0002] Vesicular stomatitis virus (VSV), which has two antigenic types of New Jersey type and Indiana type, is a zoonotic disease. Cattle, horses, and pigs are susceptible animals, and dipteran insects (such as mosquitoes and sandflies) are the main vectors. The virus content is the highest in the blister fluid, blister skin and lymph nodes of sick animals. Its clinical symptoms are indistinguishable from those of foot-and-mouth disease and swine blister disease. In recent years, there have been a variety of laboratory detection methods for VSV research, such as: virus isolation test (VI), serum neutralization test (SN), virus neutralization test (VN), enzyme-linked immunosorbent assay (direct type, Indirect type, competitive type, ELIS...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68C12N15/11
CPCC12Q1/6844C12Q1/701C12Q2531/119C12Q2563/107
Inventor 罗宝正薄清如邵建宏赵宇清沙才华廖秀云陈轩黄海超
Owner 珠海出入境检验检疫局检验检疫技术中心
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