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Culture medium for stem cell culture

A stem cell culture and culture medium technology, applied in the field of stem cell culture, can solve the problems of time-consuming and laborious culture process, cumbersome preparation method, etc., and achieve high scientific research and medical application value, strong stem cell differentiation ability, and good sealing effect.

Pending Publication Date: 2020-03-20
广州海润康华生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a medium for stem cell culture, which has the advantages of simple preparation, convenient preparation by staff, and good reproduction effect, and solves the problems of cumbersome preparation methods for stem cell culture and time-consuming and laborious cultivation process in the existing market.

Method used

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  • Culture medium for stem cell culture

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Embodiment Construction

[0018] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work shall fall within the protection scope of the present invention.

[0019] In the description of the invention, it should be noted that the terms "upper", "lower", "inner", "outer", "front end", "rear end", "both ends", "one end", "other end", etc. The indicated orientation or positional relationship is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or imp...

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Abstract

The invention discloses a culture medium for stem cell culture. The culture medium comprises a bottom protecting cover, wherein a culture medium body is arranged in the cavity of the bottom protectingcover, a top protecting cover is meshed with the top of the bottom protecting cover, buckling grooves which are in interaction for use are formed in two sides of the top of the bottom protecting cover and the bottom of the top protecting cover, and the bottom protecting cover and the top protecting cover are made from acrylic materials; the culture medium body comprises 15% of FBS, 116.6mg / L anhydrous calcium chloride, 59.05mg / L L-leucine, 0.042mg / L linoleic acid, 0.0013mg / L anhydrous copper sulfate, 91.25mg / L L-lysine hydrochloride, 0.105mg / L lipoic acid, 0.05Lmg / L iron(III) nitrate nonahydrate, 17.24mg / L L-methionine, 8.1mg / L phenol red, 0.417mg / L of ferrous sulfate heptahydrate, 35.48mg / L L-phenylalanine, and 0.081mg / L 1,4-diaminobutane dihydrochloride. The preparation method is simple, few material instruments are used, stem cells can be massively prepared for culture, the amplification speed of the stem cells is high, the stem cell differentiation capacity is high, and the stem cells can be differentiated into multiple functional cells to achieve high scientific research and medical application value.

Description

Technical field [0001] The invention relates to the technical field of stem cell culture, in particular to a culture medium for stem cell culture. Background technique [0002] Stem cells are a new research topic that is generally concerned by the biomedical community in the 21st century. Stem cells are a type of multipotent cells with self-replication ability. Under certain conditions, they can differentiate into a variety of functional cells, which are classified according to the developmental stage of stem cells. Embryonic stem cells and adult stem cells are divided into three categories according to their developmental potential: totipotent stem cells, pluripotent stem cells and unipotent stem cells. Stem cells are underdifferentiated and immature cells that can regenerate various tissues and organs and the human body. The potential function is called "universal cell" in the medical field. The stem cell culture medium acts on the special culture environment needed in the proc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12N5/0735C12N5/074
CPCC12M23/22C12M23/38C12M37/04C12N5/0606C12N5/0607C12N2500/14C12N2500/84C12N2500/32C12N2500/30C12N2500/20C12N2500/24C12N2500/46C12N2500/12C12N2500/40C12N2500/38C12N2501/115C12N2501/23
Inventor 梁勇张首平谢海博
Owner 广州海润康华生物科技有限公司
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