115 results about "Reverse Transcription Loop-mediated Isothermal Amplification" patented technology

Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a technique for the amplification of RNA. Within the last 10 years of its development, applications of the LAMP method in pathogenic microorganisms, genetically modified ingredients, tumor detection, and embryo sex identification have been widely used. This method was then improved by taking it a step further and combining it with a reverse transcription phase to allow for the detection of RNA. RT-LAMP is a one step nucleic acid amplification method that is used to diagnose infectious disease caused by bacteria or viruses. Although it has not been formally recognised by NAT, the method has been developed into many commercial kits that can be used for the identification of pathogens. The commonly used PCR method is able to generate millions of copies of the target strand. This process relies on thermal cycling, cycles of heating and cooling to facilitate the DNA replication. RT-LAMP does not require these cycles and is performed at a constant temperature between 60 and 65 °C. Similar to RT-PCR, RT-LAMP uses reverse transcriptase to synthesize complementary DNA (cDNA) from RNA sequences. This cDNA is then amplified using DNA polymerase, generating 10^9 copies per hour.