Reverse transcription-loop-mediated isothermal amplification (RT-LAMP) visual detection kit for H1N2 avian influenza viruses

Abstract

The invention discloses a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) visual detection kit for H1N2 avian influenza viruses. The kit comprises a set of LAMP primers for detecting or assisting to detect the H1 avian influenza viruses and N2 avian influenza viruses. The primers consist of a degenerate primer FIP-H1, a degenerate primer BIP-H1, a degenerate primer F3-H1, a degenerate primer B3-H1, a degenerate primer LF-H1, a degenerate primer LB-H1, a degenerate primer FIP-N2, a degenerate primer BIP-N2, a degenerate primer F3-N2, a degenerate primer B3-N2, a degenerate primer LF-N2 and a degenerate primer LB-N2. The nucleotide sequences of the primers are shown by a sequence 1, a sequence 2, a sequence 3, a sequence 4, a sequence 5, a sequence 6, a sequence 7, a sequence 8, a sequence 9, a sequence 10, a sequence 11 and a sequence 12 in a sequence table. The RT-LAMP visual detection kit for the H1N2 avian influenza viruses has the advantages that the specificity is strong, the sensitivity is high, instrument equipment and operations are simple, and results can be conveniently observed. The RT-LAMP visual detection kit for the H1N2 avian influenza viruses is especially suitable for performing rapid diagnosis and early screening on the H1 avian influenza viruses and the H1N2 avian influenza viruses in basic-level veterinary stations, farms and frontier ports.

Description

technical field [0001] The invention relates to an H1N2 subtype avian influenza virus RT-LAMP visual detection kit. Background technique [0002] Avian influenza (Avian Influenza, AI) is the abbreviation of avian influenza, which is an infectious disease of poultry (poultry and wild birds) caused by the Orthomyxoviridae influenza virus type A avian influenza virus. According to the antigenicity of the surface glycoprotein (Hemagglutinin, HA) and neuraminidase (Neuraminidase, NA) of type A influenza virus, avian influenza virus (AIV) can be further divided into different subtypes, and 16 types of HA have been found so far. subtype and nine NA subtypes. AIV is an enveloped, single-stranded negative-strand, segmented RNA virus, and the viral genome consists of eight segmented negative-strand RNAs. The segmented nature of the AIV genome makes it possible that when two or more different subtypes of influenza viruses co-infect a cell, the nucleic acid fragments of the progeny vi...

AI Technical Summary

Problems solved by technology

The traditional chicken embryo isolation method of virus is accurate and simple, but it takes too long, usually takes two days to a week; IFA and ELISA are fast but require special reagents; RT-PCR, RRT-PCR, Gene chips detection technology is fast and sensitive , but all require special instruments and technical personnel, and the cost is relatively high, so it is not suitable for promotion and use at the grassroots level

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