RT-LAMP (Reverse Transcription Loop-mediated Isothermal Amplification) nucleic acid test strip kit for detecting Japanese B encephalitis virus and application of kit

Abstract

The invention discloses an RT-LAMP (Reverse Transcription Loop-mediated Isothermal Amplification) nucleic acid test strip kit for detecting a Japanese B encephalitis virus and an application of the kit. The RT-LAMP nucleic acid test strip kit comprises a primer group and a nucleic acid detection test strip, wherein nucleotide sequences of the primer group are as shown in SEQ ID NO.1-6. A use method of the RT-LAMP nucleic acid test strip kit comprises the following steps that an RT-LAMP reaction system is prepared to carry out a thermostatic reaction; a product obtained through the thermostatic reaction is detected by using the nucleic acid detection test strip and then directly interpreted; a positive result is that two red strips appear, wherein one red strip is positioned in a detection area, and the other red strip is positioned in a quality control area. The RT-LAMP nucleic acid test strip kit disclosed by the invention has the advantages of easiness for operation, low cost, easiness for observation of reaction result and good specificity, is very suitable for export quarantine, food sanitation and site detection of a livestock cultivation farm and is easy to popularize and apply at a large range.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an RT-LAMP nucleic acid test strip kit for detecting Japanese encephalitis virus and its application. Background technique [0002] Japanese encephalitis (JE) is an important mosquito-borne zoonotic disease caused by Japanese encephalitis virus (JEV). JEV is one of the main pathogens that cause reproductive disorders in sows. The lethality rate to pigs is not high, but it can cause abortion, stillbirth or mummified fetuses in pregnant sows, and it can also cause acute inflammatory reactions or infertility in the testes of boars. Cause greater economic loss to the sustainable development of pig industry. Therefore, the control of pig JE can not only reduce the loss of pig industry, but also has great significance for the healthy development of human beings. [0003] The epidemic JE virus was first isolated from the brain tissue of patients in Japan in 1953, so it was called Japanese...

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Problems solved by technology

However, the application of this method is limited due to the shortcomings of high false positive rate, inaccurate discrimination of weak positive results, and unstable concentration ratio system of calcein and manganese ions.

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