Coxsackie virus A16 type RT-LAMP (reverse transcription-loop-mediated isothermal amplification) nucleic acid assay kit

Abstract

The invention relates to the field of biological assay technical application, and in particular relates to a coxsackie virus A16 type RT-LAMP (reverse transcription-loop-mediated isothermal amplification) nucleic acid assay kit. The kit comprises RT-LAMP reaction solutions containing four RT-LAMP primers, as well as 10000X SYBR Green I dye. The kit has the characteristics of good specificity, high sensitivity, simple operation method, quick assay, easily judged result, low cost and the like, can well fulfill the current urging requirement for field assay of hand-foot-and-mouth disease, can beused for field quick assay of disease prevention and control institutions, hospitals and kindergartens, is easy for large-scale popularization and application in grass-roots places, and has wide market prospect and great economical and social benefit.

Description

technical field [0001] The invention relates to the application field of biological detection technology, in particular to primers and kits for nucleic acid detection of various samples of Coxsackievirus A16 (feces, anal swab, throat swab, herpes fluid, cerebrospinal fluid). Background technique [0002] Coxsackievirus A16 (Coxsackievirus A16, referred to as CA16) is a single-stranded positive-sense RNA virus belonging to the family Picoranviridae. CA16 and Enterovirus 71 (EV71) are both children's hand, foot and mouth disease ( The two main pathogens of hand-foot-mouth disease (HFMD). In addition to HFMD, EV71 infection can also cause more serious complications, such as aseptic encephalitis, meningitis, pulmonary edema, etc. Compared with EV71, CA16 The symptoms of HFMD caused by infection are mild, and the incidence of complications is also low, because its subclinical infection is often ignored. However, more and more reports in recent years have pointed out that CA16 inf...

AI Technical Summary

Problems solved by technology

At present, the classic methods for detecting enteroviruses mostly use cell separation and culture and specific antibody detection. This method has cumbersome steps and a long detection cycle, and some enteroviruses are difficult to proliferate in cells, and are prone to missed detection; based on CA16 nucleic acid amplification Increased molecular biology diagnostic techniques such as PCR, RT-PCR, and real-time fluorescent quantitative RT-PCR (real-time fluorescent RT-PCR) play an important role in the detection of pathogenic microorganisms and disease diagnosis, but these methods may require precision Temperature control equipment and advanced and complex analytical instruments, or relatively high requirements for operator proficiency and professional level, and long reaction time, are not conducive to on-site sample detection, are not conducive to promotion at the grassroots level, and cannot meet simple, fast and accurate diagnosis The key to the prevention, control and treatment of epidemic diseases lies in the rapid, accurate and early detection and diagnosis of pathogenic microorganisms

Images