7 results about "Hog cholera virus" patented technology

The invention discloses a swine fever and porcine parvovirus bivalent subunit vaccine and a preparation method thereof. The vaccine comprises a first recombinant protein encoded by a first gene, a second recombinant protein encoded by a second gene and a pharmaceutically acceptable carrier. The first gene has a sequence as shown in SEQ ID NO: 1 or an increased or reduced sequence thereof. And the second gene has a sequence as shown in SEQ ID NO: 2 or an increased or reduced sequence thereof. An antigen E2 protein of a hog cholera virus (CSFV) and a VP2 protein of a porcine parvovirus (PPV) are taken as double targets, a recombinant SC-E2 protein with a SpyCatcher tag and a recombinant ST-VP2 protein with a SpyTag tag are respectively expressed in insect cells through a recombinant baculovirus vector, double-antigen covalent assembly is realized in vitro, and the constructed bivalent subunit vaccine can be used for simultaneously preventing and controlling two epidemic diseases and has a good application prospect. And the vaccine has the advantages of high safety, strong immunogenicity, high prevention and control efficiency, easiness in large-scale production and the like.

The invention belongs to the technical field of animal pathogen molecular biology, and particularly relates to a dual quantitative fluorescent primer probe composition, a kit and an identification method for identifying hog cholera virus. Aiming at the current popular strains covering 3 genotypes, 11 gene subtypes and the like of the hog cholera gene, the invention selects 5UTR and NS3 gene sequences of hog cholera virus to design and synthesize specific primers and probes aiming at the two fragments; the specific primer has no non-specific amplification on African swine fever virus, pseudorabies virus, porcine reproductive and respiratory syndrome virus, porcine circovirus type II, porcine epidemic diarrhea and bovine viral diarrhea. The invention establishes a method for identifying the classical swine fever virus wild strain and the vaccine strain and develops a kit convenient for diagnosis, and the kit and the identification method can simultaneously identify the classical swine fever virus wild strain and the vaccine strain, have the characteristics of high sensitivity, strong specificity, good repeatability and good stability, and have extremely strong application prospects.

The invention discloses a primer probe combination and a kit for fluorescent quantitative PCR (Polymerase Chain Reaction) detection of African swine fever virus and application of the primer probe combination and the kit. The primer probe combination is a specific primer probe combination 1 or a specific primer probe combination 2. The nucleotide sequence of the specific primer probe combination 1 is as shown in SEQ ID NO.1-3, and the nucleotide sequence of the specific primer probe combination 2 is as shown in SEQ ID NO.4-6. The qPCR detection method with strong specificity and high sensitivity is established, the lower detection limit can reach 0.87 copies / L, the sensitivity of the qPCR detection method is equivalent to that of a WOAH recommendation method, and the detection rate of weak positive samples is higher; the kit has no cross reaction with common swine pathogens such as hog cholera virus and pseudorabies virus, and has good specificity. The method provides reliable technical support for early diagnosis and precise prevention and control of African swine fever.

The invention discloses a classical swine fever virus E2 protein labeled subunit vaccine and a serological differential diagnosis kit matched with the classical swine fever virus E2 protein labeled subunit vaccine. The invention belongs to the field of medical preparations, and relates to a classical swine fever virus E2 protein labeled subunit vaccine and a serological differential diagnosis kit matched with the classical swine fever virus E2 protein labeled subunit vaccine. Wherein the hog cholera virus recombinant E2 protein is obtained by mutating the epitope of the E2 protein of a hog cholera virus vaccine strain LPC, and the amino acid sequence of the mutant protein is SEQ ID No: 1 or a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of the SEQ ID No: 1. The E2 marked subunit vaccine prepared by using the recombinant E2 protein has good immunogenicity, the diagnostic kit is prepared by using the E2 protein of the classical swine fever virus vaccine strain LPC and the monoclonal antibody of the DIDA55 recognition epitope of the classical swine fever virus E2 protein, and serological identification of vaccination of pigs without the marked vaccine or wild virus infection is realized, so that purification of classical swine fever is promoted.

The invention relates to the technical field of biology, in particular to a hog cholera virus E2 protein mutant and application thereof. The hog cholera virus E2 protein mutant comprises the following amino acid mutation sequence (1) or (2): (1) the hog cholera virus E2 protein is obtained by mutating the 15th site and the 25th site of the hog cholera virus E2 protein amino acid sequence; (2) mutating the 172nd site and the 323rd site of the amino acid sequence of the hog cholera virus E2 protein; wherein the amino acid sequence of the hog cholera virus E2 protein is as shown in SEQ ID NO. 1. The expression quantity of the hog cholera virus E2 protein mutant disclosed by the invention can reach 117-134 mu g / mL; the immune effect of the E2 protein mutant is better than that of the original sequence, and the positive rate of the E2 protein mutant after mice are immunized for 28 days reaches 100%.

The application discloses a CaO@SiO2 / multi-walled carbon nanotube composite material, a preparation method and application, and the preparation method comprises the following steps: step 1: CaO nanoparticles are dispersed in a solvent, a silicon source is added, and the CaO@SiO2 nanocomposite material is obtained through sufficient stirring reaction; and step 2: the CaO@SiO2 nanocomposite material and the multi-walled carbon nanotube are dispersed in a solvent, and the colloidal solution is obtained through ultrasonic mixing, and the colloidal solution is the required CaO@SiO2 / multi-walled carbon nanotube composite material; the electrochemical sensor prepared from the composite material obtained by the application can specifically detect hog cholera virus, the binding capacity of the composite material and a virus specific recognition element is improved, the interference of impurities such as impurities in a sample matrix is reduced, and the detection performance is excellent.

The invention belongs to the field of biology, and particularly relates to a PK-15 suspension cell line capable of efficiently breeding porcine circovirus and hog cholera virus, the PK-15 suspension cell line is proposed to be named as a porcine kidney cell suspension cell line in taxonomy, is preserved in Guangdong Microbial Culture Collection Center, has a preservation number of GDMCC NO.66284, is preserved on May 19, 2025, and has a preservation number of CGMCC NO.66284. The PK-15 suspension cell line can be used for efficiently breeding porcine circovirus and hog cholera virus. The preservation address is the fifth floor of building 59, No.100 courtyard, Xianlie Middle Road, Guangzhou City, Guangdong Province. The cell line is obtained through domestication by a suspension method originally created by the applicant, is named as PK15-WS04, and is preserved in Guangdong Microbial Culture Collection Center. After the PK-15 suspension cell line is subjected to 50 generations of passage, cells of each generation grow aseptically and are free of exogenous virus pollution, and the sensitivity of the PK-15 suspension cell line to porcine circovirus and hog cholera virus is not reduced; the immunogenicity of the virus prepared by each generation of cells is stable; the cell line can be used as a reserve cell line for vaccine production. Meanwhile, the invention also discloses a related application of the PK-15 suspension cell line.