46results about How to "Doubly magnified" patented technology

The invention discloses a self-assembled nucleic-acid aptamer/protein composite nano probe, a preparation method, a kit and application thereof. According to the invention, three DNA probes, one of which is a DNA probe containing a nucleic-acid aptamer sequence and another is marked with horseradish peroxidase, are hybridized to prepare a self-assembled nucleic-acid aptamer/protein composite nanoprobe; and magnetic nano-particles functionalized by a nucleic-acid aptamer are used as magnetic separation solid-phase carriers for specific capture of human acute lymphoblastic leukemia T lymphocytes (CCRF-CEM) in blood, to improve the capture efficiency of target substances. When the magnetic nano-particles are used in combination with the self-assembled nucleic-acid aptamer/protein composite nano probe, the detection of CCRF-CEM has a wide linear range, a low detection limit, and high precision and accuracy, and high-sensitivity, high-specificity and rapid detection of CCRF-CEM in peripheral blood samples can be realized, which has great significance for auxiliary diagnosis, curative effect evaluation and prognosis judgment of leukemia.

The invention discloses a preparation method of a bifunctional enzyme-free hydrogen peroxide sensor, which is constructed on the basis of a functional nano material. The prepared sensor has the advantages of simple operation, portability, rapidness for detection, and low cost, and can be used to rapidly and sensitively detect hydrogen peroxide in daily life and production.

The invention discloses a preparation method for an immune-biological sensor constructed on the basis of a functional nanometer material and with an electrochemiluminescence and photoelectrochemical dual signal amplification strategy. The prepared sensor is easy to operate, convenient to carry, high in detection speed and low in cost, and can be used for rapidly and sensitively detecting ethinylestradiol in the fields of daily production and living and the like.

The invention discloses a preparation method of a functional nanomaterial based biological immunosensor with dual signal sending strategies of electrochemiluminescence and photoelectrochemistry.The sensor prepared according to the method has the advantages of simplicity in operation, portability, quickness in detection and low in cost and can be used for rapid and sensitive detection of deltamethrin in fields of daily production, daily life and the like.

The invention relates to a composite hollow microsphere material for a biosensor and a preparation method thereof and belongs to the field of biosensor preparation. The preparation method comprises pretreating a cellulose raw material with an alkali solution, immersing the cellulose raw material in an oxidizing salt solution, carrying out hydrolysis to obtain carboxylated cellulose nanospheres, dispersing the carboxylated cellulose nanospheres in water, adding an organic solvent into the solution, carrying out uniform stirring, adding zinc nitrate hexahydrate into the solution, adding an alkali into the solution drop by drop for a reaction to obtain zinc oxide precursor-cellulose composite microspheres, dispersing the zinc oxide precursor-cellulose composite microspheres in an aqueous solution of graphene oxide to obtain zinc oxide precursor/graphene oxide cellulose composite microspheres, carrying out calcination at a high temperature and removing cellulose to obtain the composite hollow microsphere material. The composite hollow microsphere material is used for a biosensor and significantly increases the sensitivity and stability of the sensor.