Preparation method and use of Pd/V2O5/MWCNTs-based intestinal cancer tumor marker immunosensor

A technology of tumor markers and immune sensors, applied in the field of immune analysis and biosensing detection, and new functional materials, can solve the problems of cumbersome detection methods, high detection limit, and complicated operation, and achieve fast detection speed and wide detection range , the effect of simple operation

Inactive Publication Date: 2015-10-28
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are many clinical detection methods for cancer markers, such as radioimmunoassay, immunoradiometric assay, enzyme-labeled immunoassay, chemiluminescence assay, time-resolved fluorescence immunoassay, etc., but most of the detection methods are cumbersome, The operation is complicated, the cost is expensive, and the detection limit is high. Therefore, it is of great significance to establish a fast, simple and sensitive detection method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1. A Pd / V based 2 o 5 The preparation method of the intestinal cancer tumor marker immunosensor of MWCNTs is characterized in that the steps are as follows:

[0046] (1) A glassy carbon electrode with a diameter of 3 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water;

[0047] (2) Drop-coat 6 μL, 1.0 mg / mL tin dioxide-doped graphene solution on the surface of the electrode, let it dry, rinse it with ultrapure water, and let it dry;

[0048] (3) Drop-coat 6 μL of gold nanoparticle solution on the surface of the electrode, let it dry, rinse it with ultrapure water, and let it dry;

[0049] (4) Drop-coat 6 μL, 8 μg / mL colorectal cancer tumor marker capture antibody Ab 1 solution on the electrode surface, 4 o C dry in refrigerator;

[0050] (5) Rinse away unbound capture antibody Ab with ultrapure water 1 After that, drop 6 μL of 0.1% bovine serum albumin BSA solution on the surface of the electrode, 4 o C to dry in...

Embodiment 2

[0053] Example 2. A Pd / V based 2 o 5 The preparation method of the intestinal cancer tumor marker immunosensor of MWCNTs is characterized in that, the steps are as follows:

[0054] (1) A glassy carbon electrode with a diameter of 4 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water;

[0055] (2) Drop-coat 6 μL, 1.5 mg / mL tin dioxide-doped graphene solution on the surface of the electrode, let it dry, rinse it with ultrapure water, and let it dry;

[0056] (3) Drop-coat 6 μL of gold nanoparticle solution on the surface of the electrode, let it dry, rinse it with ultrapure water, and let it dry;

[0057] (4) Drop-coat 6 μL, 10 μg / mL colorectal cancer tumor marker capture antibody Ab 1 solution on the electrode surface, 4 o C dry in refrigerator;

[0058] (5) Rinse away unbound capture antibody Ab with ultrapure water 1 After that, drop 6 μL of bovine serum albumin BSA solution with a mass fraction of 0.5% on the surface of the e...

Embodiment 3

[0061] Example 3. A Pd / V based 2 o 5 The preparation method of the intestinal cancer tumor marker immunosensor of MWCNTs is characterized in that, the steps are as follows:

[0062] (1) A glassy carbon electrode with a diameter of 5 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water;

[0063] (2) Drop-coat 6 μL, 2.0 mg / mL tin dioxide-doped graphene solution on the surface of the electrode, let it dry, rinse it with ultrapure water, and let it dry;

[0064] (3) Drop-coat 6 μL of gold nanoparticle solution on the surface of the electrode, let it dry, rinse it with ultrapure water, and let it dry;

[0065] (4) Drop-coat 6 μL, 12 μg / mL colorectal cancer tumor marker capture antibody Ab 1 solution on the electrode surface, 4 o C dry in refrigerator;

[0066] (5) Rinse away unbound capture antibody Ab with ultrapure water 1 After that, drop 6 μL of 1.0% bovine serum albumin BSA solution on the surface of the electrode, 4 o C to dry ...

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Abstract

The invention belongs to the technical field of nanometer functional materials, immunization analysis and biosensing and provides a preparation method and use of a Pd / V2O5 / MWCNTs-based intestinal cancer tumor marker immunosensor. Through use of Pd / V2O5 / MWCNTs as a detection antibody marker, a sandwiched electrochemical immunosensor for detecting an intestinal cancer tumor marker is prepared. Through excellent biocompatibility and high catalytic performances of Pd / V2O5 / MWCNTs, the immunosensor has the advantages of high specificity, high sensitivity and low detecting limit.

Description

technical field [0001] The present invention relates to a kind of based on Pd / V 2 o 5 Preparation method and application of intestinal cancer tumor marker immunosensor of MWCNTs. Specifically using Pd / V 2 o 5 / MWCNTs is used as a detection antibody marker to prepare a sandwich-type electrochemical immunosensor for the detection of intestinal cancer tumor markers, which belongs to the technical fields of new functional materials, immune analysis and biosensing detection. Background technique [0002] Colon cancer is a common malignant tumor in the gastrointestinal tract, and its morbidity and mortality have increased significantly in recent years. Early diagnosis of intestinal cancer can greatly increase the cure rate and improve the quality of life of patients. In clinical research, it is very important to develop a fast, simple and sensitive method for detecting tumor markers of intestinal cancer. [0003] At present, there are many clinical detection methods for cance...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/543
CPCG01N33/54346G01N33/5438G01N33/57419
Inventor 李月云王平韩健董云会刘青陈磊
Owner SHANDONG UNIV OF TECH
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