49results about How to "Good molecular recognition" patented technology

The invention relates to a preparation method of a bionic artemisinin molecular imprinting composite membrane, belonging to the technical field of environmental material preparation. The preparation method comprises the following specific steps: by taking a regenerated cellulose membrane as a substrate, artemisinin as a template molecule, acrylamide (AM) as a functional monomer and ethylene glycol dimethacrylate (EGDMA) as a cross-linking agent, inlaying silicon dioxide nanoparticles with functional monomer modified surfaces to the surface of a membrane material by combination with a dopamine bionic principle, and performing two-step temperature polymerization to prepare the bionic artemisinin molecular imprinting composite membrane. The static adsorption experiment is used for making a research on the adsorption balance, dynamics and selective recognition performance of the prepared imprinting membrane. A result shows that the artemisinin imprinting membrane prepared by adopting the method is relatively high in adsorption capacity and superior in artemisinin molecular recognition penetration performance.

A preparation method for molecular recognition sensor by electrodeposition is provided. The preparation method is as following: forming molecularly imprinted polymeric micelles by self-assembly of ionic type photosensitive copolymers; forming a film on the surface of an electrode by electrodepositing the molecularly imprinted polymeric micelles at a constant potential; crosslinking the electrodeposited micellar film via ultraviolet light irradiation; extracting the template molecules from the crosslinked film to obtain electrode modified by the molecularly imprinted polymeric micellar film; and connecting the modified electrode with a sensor device and a computer to construct a molecular recognition sensing system capable of specifically detecting the template molecules.

The invention relates to a preparation method of an arteannuin molecularly imprinted composite membrane, belonging to the technical field of environmental material preparation. The method comprises the following steps: by using a regenerated cellulose membrane as a substrate, the arteannuin as a template molecule, acrylamide (AM) as a functional monomer and ethylene glycol dimethyl acrylate (EGDMA) as a crosslinking agent, cuprous bromide and N,N',N'',N''-pentamethyldiethylenetriamine constitute a catalytic system, and an atom transfer free-radical polymerization process is utilized to prepare the arteannuin molecularly imprinted composite membrane. A static adsorption experiment is used for researching the adsorption equilibrium, dynamics and selective recognition performance of the imprinted membrane; and the result proves that the arteannuin imprinted membrane has fast adsorption dynamical properties and excellent arteannuin molecule recognition performance.

The invention relates to a method for preparing an apiolin molecularly imprinted polymer, comprising the following steps: dissolving apiolin and a functional monomer of 1 mol fraction of template molecule according to the mole ratio of 1:4-1:10 in 40 parts by volume of butylene oxide of a pore-foaming agent; vibrating away from light at constant temperature for 6h; then adding ethylene dimethacrylate as a crosslinking agent, the amount of which is 4-7 times of that of the monomer, and azodiisobutyronitrile as an initiating agent, the quality of which is 1 percent of that of the monomer and thecrosslinking agent, and completely mixing; sealing after N2 is introduced to remove oxygen; carrying out thermal-initiated polymerization at the temperature of 40-60 DEG C for 24-48h, and then obtaining a rod-shaped solid polymer; rubbing and then sieving the polymer; utilizing a methanol/acetic acid (v/v=6:4) eluent to elute so as to remove the template molecule; putting in a vacuum drying cabinet and then drying until constant weight, and then obtaining the apiolin molecularly imprinted polymer. The apiolin molecularly imprinted polymer prepared by the method has specific identification ability and selective adsorption performance on apiolin. Compared with common sorbents, the polymer has the advantages of accuracy, rapidness and sensitivity. Moreover, the polymer has high percent recovery, good repeatability and long service life, thereby having wide application prospects.

A process for preparing microspheres of molecular blot polymer in water medium includes such steps as dissolving ethoxyl cellulose in water while stirring, putting functional monomer in water, mixing trimethoxyl propane, trimethyl acrylate, trigger and diluent together, mixing said three solutions together, introducing nitrogen gas, sealing, stirring at 65-80 deg.C and 300-400 rpm for 6-10 hr, washing with organic solvent, eluting with diluted acid until temp moleculae are removed, washing with water until it shows neutral, and drying in air.

The invention relates to a preparation method of nanometer silicon gel surface gastrodin molecular engram polymers, which belongs to the technical field of biochemical engineering. The preparation method of the invention comprises the following steps: dissolving template molecules of gastrodin and functional monomers of alpha-methacrylic acid into hole forming agents of methanol; sufficiently mixing the materials under the conditions of constant temperature and light shielding to obtain mixed liquid; adding silanization nanometer silicon gel particles, cross linking agents and catalytic amount triggering agents into the mixed liquid; carrying out repeated ultrasonic treatment after sufficient and uniform mixing; introducing nitrogen gas for deoxygenation; sealing the materials; carrying out reaction for 6h in water bath of 50 DEG C; then, carrying out reaction for 24h at 60 DEG C to obtain solid polymers; adopting a Sohxlet extraction method for extracting the solid polymers for more than 48h; carrying out elution for removing template molecules; drying the polymers till the constant weight after the extraction is completed; and obtaining the silicon gel surface gastrodin molecular engram polymers. The molecular engram polymers prepared by the invention have specific identification capability and selective absorption performance on the gastrodin. Compared with ordinary absorping agents, the molecular engram polymers prepared by the invention have the advantages of accuracy, high speed and sensitivity.

The invention discloses an amphiphilic polysaccharide ppma which can be degraded biologically, a relative preparation method, a micellization route of the polysaccharide ppma in selective solvent, a nanometer micelle drug carrier and a drug release route in vitro. The preparation method comprises synthesizing 1-methyl-3-butyl imidazole bromonium ionic liquid, using stannous octoate as catalyst to catalyze the ring-opening polymerization reaction of dextran and caprolactone in the ionic liqud, to obtain the amphiphilic polysaccharide ppma which can be degraded biologically, as dextran amphiphilic ppma containing polycaprolactone chain. The ppma can form stable micelle in selective solvent and can prepare the micelle for carrying indenemethyl drug. The amphiphilic polysaccharide ppma which can be degraded biologically has the ability for self-assembling micelle in selective solvent and loading ability for hydrophobic drug. And the product has the advantages of chemical modification, biodegradability and biocompatibility, with wide application in biological medicine technical field.

The invention relates to a method for preparing a molecularly-imprinted monolithic column by using a molecular crowding reagent and ionic liquid as a pore-foaming agent. The method comprises the following steps: through special formulation of the pore-foaming agent, adding a template molecule ursolic acid or oleanolic acid into a pore-foaming agent consisting of a macromolecular crowding reagent, the ionic liquid and dimethyl sulfoxide, adding a functional monomer, a cross-linking agent and an initiator, performing ultrasonic degassing, and introducing nitrogen for removing oxygen to obtain an ursolic acid or oleanolic acidmolecularly-imprinted monolithic column. By the method, the macromolecular reagent and the green solvent ionic liquid can form the molecularly-imprinted monolithic column with good permeability and a significant imprinting effect; the imprinting effect of the obtained ursolic acid or oleanolic acidmolecularly-imprinted monolithic column is better than that when the macromolecular reagent and the ionic liquid are independently used as the pore-foaming agent; the method has the characteristics of simple preparation, avoidance of a complicated preparation process, good molecular recognition performance and the like; the obtained molecularly-imprinted monolithic column has good permeability and a significant imprinting effect; a new method is provided for preparing the molecularly-imprinted monolithic column with better imprinting effect.

The invention relates to a glycosyl functional bacterial toxin molecularly imprinted membrane substrate as well as a preparation method and an application thereof. The molecularly imprinted membrane substrate forms a reaction layer on the surface of a gold quartz crystal substrate by taking a glycosyl functional bacterial toxin molecularly imprinted polymer as a recognition element. The preparation method comprises the following steps: selecting a carbohydrate molecule functional monomer which can carry out specificity recognition with bacterial toxin and compound the corresponding molecularly imprinted polymer; preparing a glycosyl functional molecularly imprinted polymer solution; and modifying the glycosyl functional molecularly imprinted polymer on the surface of the gold quartz crystal substrate by a substrate surface modifying technology to form a reaction layer. The glycosyl functional molecularly imprinted membrane substrate prepared by the method is connected to a piezoelectric quartz crystal microbalance to detect bacterial toxin in an environmental sample extracting solution. The molecularly imprinted membrane substrate has favorable molecular recognition performance and greatly improves the sensitivity and the selectivity for detecting the bacterial toxin.

The invention discloses an organic arsenide molecularly imprinted membrane substrate in aptamer-based marine products, and a production method and application thereof, wherein an organic arsenide molecularly imprinted polymer in the aptamer-based marine product is taken as a recognition element to form a reaction layer on the surface of a gold quartz body substrate; and the organic arsenide molecularly imprinted polymer in the aptamer-based marine product is formed by polymerizing organic arsenide template molecule, aptamer functional monomer, cross-linking agent, porogen, initiating agent and organic solvent in the marine product according to the molar ratio of (0.1-2.5):3:(0.1-3):(30-60):(0.01-0.15):(1.0-10). The surface finish technology is applied to the preparation of the organic arsenide molecularly imprinted membrane substrate in the aptamer-based marine product, so the preparation of the organic arsenide molecularly imprinted membrane substrate in the aptamer-based marine product has controllability, and the sensitivity and the accuracy of the substrate are improved.

The invention discloses an open-ring cucurbituril column aromatic hydrocarbon double-main-body compound and a preparation method thereof. According to the ring-opening cucurbituril column aromatic hydrocarbon double-main-body compound, haloalkane modified ring-opening cucurbituril reacts with carboxyl on carboxylic acid modified column aromatic hydrocarbon to form an ester bond, and the ester bondis linked into the double-main-body compound; compared with single open-ring cucurbituril and pillar arene, the open-ring cucurbituril column aromatic hydrocarbon double-main-body compound has two cavities with different properties, one cavity is a pillar arene cavity, the other cavity is an open-loop cucurbituril cavity, the two cavities are arranged in a linear structure, and the cavities of the carrier have different properties. The compound has the properties of both pillar arene and open-ring cucurbituril, has very good molecular recognition capability, can be used as a molecular ion transmembrane transport molecule, and is applied to the industries of biomedical research, pharmacy, food, essence and flavor, tobacco and the like.

The invention discloses a common and convenient epitope imprinting method. The method comprises the following steps: firstly, selecting terminal polypeptide of target protein as a characteristic epitope; carrying out saccharification treatment to obtain saccharified polypeptide and taking the saccharified polypeptide as an imprinting template; anchoring the imprinting template on a boric acid functionalized substrate material through boron affinity interaction; then selecting monomer silylation reagents with different types and ratios and carrying out non-covalent imprinting; accurately imprinting the thickness of an imprinting layer through adjusting the imprinting time, so as to obtain a molecular imprinting polymer. The imprinting technology has the advantages of good commonality, convenience for preparation, good identification performance and the like. A molecular imprinting material prepared by the method can be used for identifying an epitope segment and also can be used for specifically identifying complete target protein, so that the molecular imprinting material has an important application prospect in the fields of separation, enrichment, purification, sensing, proteomics, target identification and the like.

The invention relates to a method for preparing an organophosphorus insecticide nano silica gel surface molecular imprinted polymer. The method comprises the following steps of: preparing nano SiO2 microspheres; performing three different kinds of surface graft modification on the produced SiO2 microspheres; preparing pre-assembled solution; ultrasonically dispersing one of the modified SiO2 microspheres in a polymer solvent; adding a crosslinking agent, an initiator and the pre-assembled solution; performing centrifugation to remove supernatant after a reaction is finished; removing template molecules from the precipitate by using the mixed solution of methanol/acetic acid; putting the polymer microspheres with the template molecules removed in a vacuum drying box; and drying the polymer microspheres at the temperature of 60 DEG C to constant weight to obtain the molecular imprinted polymer microspheres. The three different molecular imprinted polymers prepared by the method have specificity identification to an organophosphorus insecticide (dursban) and can perform selective separation and enrichment on the residues of the organophosphorus insecticide.

The invention relates to a preparation method of a surface imprinting adsorbent for identifying ciprofloxacin at high sensitivity and belongs to the technical field of preparing environmental materials, and in particular relates to a method for preparing a surface imprinting adsorbent of ciprofloxacin with active dry yeast as the substrate, ciprofloxacin (CIP) as the template molecule, acrylamide (AM) as the functional monomer, ethylene glycol dimethyl acrylate (EGDMA) as the cross-linking agent and 2,2'-azodiisobutyronitrile (AIBN) as the initiator through a precipitation polymerization process. Static adsorption experiment is carried out to study the adsorption balance, dynamics and high-selectivity identification properties of the prepared imprinting adsorbent, and the result of the static adsorption experiment indicates that the surface imprinting adsorbent has fast adsorption dynamic property and excellent ciprofloxacin molecule identification property.

The invention discloses preparation methods and applications of a menthofuran molecular imprinted polymer and a monolithic column. The preparation method of the monolithic column comprises the following steps: dissolving template molecule menthofuran into a pore forming agent, separately adding a functional monomer, a cross-linking agent and an initiator for mixing, performing ultrasonic treatmentin an ice-water bath, introducing a nitrogen gas, injecting the reactants into a stainless steel chromatographic column and a container, performing sealing, putting the sealed objects into a drying oven with the temperature of 50-60 DEG C, performing a reaction for 12-24h to obtain a molecular imprinted polymer monolithic column through thermal initiation, connecting the monolithic column to a liquid chromatography pump to wash away the template molecule, so as to finally obtain the monolithic column with good separation effects. According to the methods, a frontier chromatography method is utilized to analyze the number of effective binding sites and dissociation constant of the prepared molecular imprinted polymer monolithic column and the menthofuran, the molecular recognition performance of the method is good, the molecular imprinted polymer provided by the invention is used as a liquid chromatography column filler and can realize the separation, enrichment and purification of thementhofuran in essential oil with more complex components.