The invention discloses a making method of two-layer embedded artificial seeds of
orychophragmus violaceus, and belongs to the field of
biotechnology and
agricultural engineering. The method comprises the steps of
bud cluster inducing of the
orychophragmus violaceus,
bud propagation and cultivation and
bud embedding and artificial seed
capsule preparation; in the bud embedding and artificial seed
capsule preparation process, an endopleura embedding solution is
sodium alginate
hydrosol which contains nano-
titanium dioxide nTiO2, NAA, an MS medium and saccharose and has the
mass concentration of 6%, and a testa embedding solution is a
sodium alginate
hydrosol which contains nano-
titanium dioxide nTiO2 and nano-
silicon dioxide nSiO2 and has the
mass concentration of 7% or 8%. According to the made artificial seeds, a bactericide and
nutrient substances can be separated through a two-layer embedding technology, and meanwhile the
mechanical strength of endopleura and testa is adjusted; the artificial seeds have the advantages of being high in rooting and
sprouting rate, low in
contamination rate, low in toxic action on explants and the like in a bacterial environment; by means of the two-layer embedding technology, the making technology of the artificial seeds of the
orychophragmus violaceus can be further pushed to practical production and application.