The invention discloses a telescopic and fixation-type endoscope puncture device. The telescopic and fixation-type endoscope puncture device comprises a tube cavity, a tube cavity base, a pneumoperitoneum port, a base sheet, M upper rotary buckles, M lower rotary buckles, N arc strutting pillars, N strutting petals, N strutting petal buckles, an outer core, an outer core base, N groove holes, a cutting part, an inner core, an inner core base and N rotary clamping grooves; the tube cavity comprises a tube cavity telescoping section and a tube cavity fixing section, the top of the tube cavity isconnected with the tube cavity base, and the two ends of the tube cavity telescoping section are connected with the tube cavity base and the tube cavity fixing section respectively. According to thetelescopic and fixation-type endoscope puncture device, through the arrangement of the tube cavity, the outer core, the inner core, the N arc strutting pillars, the N strutting petals, the N struttingpetal buckles and the N rotary clamping grooves, firm fixation of the interiors of the abdominal wall and the chest wall by the puncture device is achieved, and slip is avoided; meanwhile, the puncture length of the puncture device can be shortened, the situation that redundant tube cavity parts are left in the abdominal cavity and the chest cavity is avoided, convenience is brought to surgical operation, and precious time is saved.