The invention discloses an electro-hydraulic loading system of a film biaxial tensile testing machine. The electro-hydraulic loading system comprises a low-pressure filter, hydraulic pumps, a motor, a one-way valve, a stop valve, an energy storage device, an electromagnetic unloading valve, a proportional overflow valve, a pressure compensator, a proportional directional valve, a servo hydraulic cylinder, a pull pressure sensor, a displacement sensor, a mechanical fixture, a filter, a cooler and an oil tank, wherein the two hydraulic pumps share the one oil tank, and two independent loading hydraulic loops can be formed, so that the manufacturing cost and the installation space are greatly saved. Furthermore, the servo hydraulic cylinder can be controlled by the single set of pressure compensator and proportional directional valve, so that loading synchronism and stability can be guaranteed. The electro-hydraulic loading system of the film biaxial tensile testing machine has the characteristics of being simple in structure, large in tensile force, high in loading synchronism and accuracy, rapid in control response and the like, and can be used for film composite material biaxial tensile testing machines such as an architectural membrane material tester and an airship skin test as well as a uniaxial drawing tester.