The invention provides an aeroponic virus-free seed potato breeding method and equipment. The breeding method includes the following steps: equipment cleaning and disinfection, greenhouse disinfection through a smoke drug, seedling hardening, planting and atomization, illumination and temperature management, seedling shedding, overgrowth and lodging control, nutrient solution concentration management, nutrient solution pH value management, nutrient solution temperature management, pest and disease damage prevention, and harvesting. The breeding equipment includes cultivation tanks, cultivation plates, a nutrient solution, a solution storage container, a solution delivery pipe, atomization nozzles, a circulating pump, and a nutrient solution return pipe, wherein each cultivation tank is a cuboid tank of which the length is much larger than the width, the inner surface of each cultivation tank is painted black, the outer surface of each cultivation tank is painted silvery grey, and each cultivation tank is supported from the ground through a bracket; the solution storage container is an underground solution storage pool, a temperature sensor is arranged in the solution storage pool, and a nutrient solution cooling thermolator is arranged outside the solution storage pool. The method provided by the invention can reduce the seedling time, prolong the collection period, and increase the yield; the number of stolons is increased, so as to lay a foundation for a higher yield of virus-free seed potatoes. The equipment provided by the invention is convenient to manage, can save energy, and runs reliably.