The invention discloses a ventilation method and a ventilation device for culturing seedlings of plants in full-closed environment, and relates to the technical field of agricultural growing. The ventilation method comprises that: in a full-closed seedling culturing box, an upper seedling culturing chamber, a suction fan, a lower seedling culturing chamber and an exhaust fan are communicated in turn through air ducts on two sides to form an internal circulating ventilation circuit; and the suction fan drafts air and the exhaust fan exhausts air so as to form internal circulating ventilation; and the ventilation device comprises a full-enclosed seedling culturing box 32, an upper seedling culturing chamber 3, a lower seedling culturing chamber 7, a suction fan 1, an exhaust fan 2, an air conditioner 4, a CO2 supplementing tank 5, an O2 supplementing tank 6, an automatic control device 8 and the like, has the function of simulating natural ventilation in the full-closed environment seedling culturing equipment, realizes the functions of uniform ventilation, dehumidification, humidification, heating, refrigeration, supplementation of O2 and Co2 and the like of multi-layer seedling culturing frame high-density seedling culture in the full-closed environment, is suitable for culturing the seedlings of the plants in greenhouses and plastic tents in various environments, and is also suitable for growing plants in the full-closed environment.