Making method of two-layer embedded artificial seeds of orychophragmus violaceus

An artificial seed and double-layer embedding technology, which is applied in the fields of biotechnology and agricultural engineering, can solve the problems of low germination rate, restrict the development of artificial seed technology, and insufficient antibacterial ability, achieve low plant toxicity and improve germination rate. and rooting rate, good antibacterial effect

Active Publication Date: 2016-11-23
东台海滨科技创业园管理有限公司
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Problems solved by technology

[0004] At present, the single-layer embedded artificial seed technology has made some progress. The single-layer embedded Zhuge vegetable artificial seed added with nano-titanium dioxi

Method used

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  • Making method of two-layer embedded artificial seeds of orychophragmus violaceus
  • Making method of two-layer embedded artificial seeds of orychophragmus violaceus
  • Making method of two-layer embedded artificial seeds of orychophragmus violaceus

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Embodiment Construction

[0023] The technical solution of the present invention will be further described in detail below in conjunction with the embodiments.

[0024] In an embodiment of the present invention, a kind of double-layer embedded Zhuge Cai artificial seed production method comprises the following steps:

[0025] Step 1, bud cluster induction of Zhuge Cai: take Zhuge Cai seedlings, wash them with sterile water for 3 minutes, cut out the axillary buds and terminal buds, complete disinfection on the ultra-clean workbench, and inoculate aseptically on the bud cluster induction medium for bud clusters Induction culture; the composition of bud cluster induction medium includes: MS medium, 2mg / L 6-BA (6-benzylaminoadenine), 0.2mg / L NAA (α-naphthaleneacetic acid), 3% sucrose, 0.65% agar ; The light intensity of the bud induction process is 2000-3000 Lux fluorescent lamp for 12 hours.

[0026] Step 2, Bud propagation culture of Zhuge Cai: Take the Zhuge Cai tissue culture seedlings induced by the...

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Abstract

The invention discloses a making method of two-layer embedded artificial seeds of orychophragmus violaceus, and belongs to the field of biotechnology and agricultural engineering. The method comprises the steps of bud cluster inducing of the orychophragmus violaceus, bud propagation and cultivation and bud embedding and artificial seed capsule preparation; in the bud embedding and artificial seed capsule preparation process, an endopleura embedding solution is sodium alginate hydrosol which contains nano-titanium dioxide nTiO2, NAA, an MS medium and saccharose and has the mass concentration of 6%, and a testa embedding solution is a sodium alginate hydrosol which contains nano-titanium dioxide nTiO2 and nano-silicon dioxide nSiO2 and has the mass concentration of 7% or 8%. According to the made artificial seeds, a bactericide and nutrient substances can be separated through a two-layer embedding technology, and meanwhile the mechanical strength of endopleura and testa is adjusted; the artificial seeds have the advantages of being high in rooting and sprouting rate, low in contamination rate, low in toxic action on explants and the like in a bacterial environment; by means of the two-layer embedding technology, the making technology of the artificial seeds of the orychophragmus violaceus can be further pushed to practical production and application.

Description

technical field [0001] The invention belongs to the fields of biotechnology and agricultural engineering, and in particular relates to a method for making artificial seeds of zhuge kale embedded in double layers. Background technique [0002] Zhugecai, also known as Eryuelan, is a plant of the genus Zhugecai in the family Cruciferae, an annual or biennial herb, widely distributed in most parts of the country, resistant to cold, drought, and shade, and has few pests and diseases. The flowers are purple or white, and their life history is from September to June of the next year. Even in the cold winter, they can still keep green and cover the ground well. It is a rare flower in early spring and green in winter in the northern region. The ground cover plant is very popular among people. In the fatty acid composition of the seeds of Zhugecai, the content of erucic acid and linolenic acid is low, and the content of palmitic acid, oleic acid and linoleic acid is 89.8% of the tota...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/006
Inventor 吴沿友谷睿智邢德科吴沿胜姚香萍黎明鸿付为国
Owner 东台海滨科技创业园管理有限公司
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