Making method of two-layer embedded artificial seeds of orychophragmus violaceus
An artificial seed and double-layer embedding technology, which is applied in the fields of biotechnology and agricultural engineering, can solve the problems of low germination rate, restrict the development of artificial seed technology, and insufficient antibacterial ability, achieve low plant toxicity and improve germination rate. and rooting rate, good antibacterial effect
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[0023] The technical solution of the present invention will be further described in detail below in conjunction with the embodiments.
[0024] In an embodiment of the present invention, a kind of double-layer embedded Zhuge Cai artificial seed production method comprises the following steps:
[0025] Step 1, bud cluster induction of Zhuge Cai: take Zhuge Cai seedlings, wash them with sterile water for 3 minutes, cut out the axillary buds and terminal buds, complete disinfection on the ultra-clean workbench, and inoculate aseptically on the bud cluster induction medium for bud clusters Induction culture; the composition of bud cluster induction medium includes: MS medium, 2mg / L 6-BA (6-benzylaminoadenine), 0.2mg / L NAA (α-naphthaleneacetic acid), 3% sucrose, 0.65% agar ; The light intensity of the bud induction process is 2000-3000 Lux fluorescent lamp for 12 hours.
[0026] Step 2, Bud propagation culture of Zhuge Cai: Take the Zhuge Cai tissue culture seedlings induced by the...
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