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Method for preparing apiolin molecularly imprinted polymer

A technology of molecular imprinting and apigenin, which is applied in chemical instruments and methods, other chemical processes, etc., can solve the problems related to the undiscovered molecular imprinting polymer of apigenin, and achieve remarkable molecular recognition ability, broad application prospect, good heat The effect of stability

Inactive Publication Date: 2010-03-10
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] After checking the literature of the prior art, no relevant reports on the preparation of molecularly imprinted polymers of apigenin have been found so far

Method used

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  • Method for preparing apiolin molecularly imprinted polymer
  • Method for preparing apiolin molecularly imprinted polymer
  • Method for preparing apiolin molecularly imprinted polymer

Examples

Experimental program
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Effect test

example 1

[0023] Dissolve 0.5mmol template molecule apigenin and 4mmol functional monomer 2-vinylpyridine into 20.0mL porogen tetrahydrofuran to prepare a mixed solution, shake at constant temperature and avoid light for 6h, add cross-linking agent ethylene glycol dimethacrylate 20mmol, initiator azobisisobutyronitrile (the amount is 1% of the mass of the monomer and crosslinking agent), fully miscible, degassed by ultrasonic for 15min, deoxygenated by nitrogen for 15min, and sealed the tube under vacuum. React in a water bath at 60°C for 24 hours to obtain a rod-shaped solid polymer. The prepared polymer was ground and passed through a 180-mesh sieve, fine particles were removed by acetone sedimentation three times, and dried to constant weight. The mixed solution of methanol and acetic acid with a volume ratio of 6:4 was used as the extraction solution, and the polymer was Soxhlet extracted for 72 hours, and the template molecules were removed, and then washed with pure methanol for 3...

example 2

[0025] Dissolve 0.5mmol template molecule apigenin and 2mmol functional monomer acrylamide into 20.0mL porogen tetrahydrofuran to prepare a mixed solution, shake at constant temperature and avoid light for 6h, add 10mmol cross-linking agent ethylene glycol dimethacrylate, trigger Azobisisobutyronitrile (the amount used is 1% of the mass of the monomer and cross-linking agent), after fully miscible, ultrasonic degassed for 20 minutes, deoxygenated with nitrogen for 15 minutes, and the tube was sealed under vacuum. React in a water bath at 60°C for 24 hours to obtain a rod-shaped solid polymer. The prepared polymer was ground and passed through a 180-mesh sieve, fine particles were removed by acetone sedimentation three times, and dried to constant weight. The mixed solution of methanol and acetic acid with a volume ratio of 6:4 was used as the extraction solution, and the polymer was Soxhlet extracted for 72 hours, and the template molecules were removed, and then washed with p...

example 3

[0027] Dissolve 0.5mmol template molecule apigenin and 4mmol functional monomer 2-vinylpyridine into 20.0mL porogen tetrahydrofuran to prepare a mixed solution, shake at constant temperature and avoid light for 6h, add cross-linking agent ethylene glycol dimethacrylate 28mmol, initiator azobisisobutyronitrile (the amount is 1% of the mass of the monomer and the crosslinking agent), after fully miscible, ultrasonic degassing for 20 minutes, nitrogen deoxygenation for 15 minutes, and sealing the tube under vacuum. React in a water bath at 60°C for 24 hours to obtain a rod-shaped solid polymer. The prepared molecularly imprinted polymer was ground and passed through a 180-mesh sieve, fine particles were removed by acetone sedimentation three times, and dried to constant weight. The mixed solution of methanol and acetic acid with a volume ratio of 6:4 was used as the extraction solution, and the polymer was Soxhlet extracted for 72 hours, and the template molecules were removed, a...

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Abstract

The invention relates to a method for preparing an apiolin molecularly imprinted polymer, comprising the following steps: dissolving apiolin and a functional monomer of 1 mol fraction of template molecule according to the mole ratio of 1:4-1:10 in 40 parts by volume of butylene oxide of a pore-foaming agent; vibrating away from light at constant temperature for 6h; then adding ethylene dimethacrylate as a crosslinking agent, the amount of which is 4-7 times of that of the monomer, and azodiisobutyronitrile as an initiating agent, the quality of which is 1 percent of that of the monomer and thecrosslinking agent, and completely mixing; sealing after N2 is introduced to remove oxygen; carrying out thermal-initiated polymerization at the temperature of 40-60 DEG C for 24-48h, and then obtaining a rod-shaped solid polymer; rubbing and then sieving the polymer; utilizing a methanol / acetic acid (v / v=6:4) eluent to elute so as to remove the template molecule; putting in a vacuum drying cabinet and then drying until constant weight, and then obtaining the apiolin molecularly imprinted polymer. The apiolin molecularly imprinted polymer prepared by the method has specific identification ability and selective adsorption performance on apiolin. Compared with common sorbents, the polymer has the advantages of accuracy, rapidness and sensitivity. Moreover, the polymer has high percent recovery, good repeatability and long service life, thereby having wide application prospects.

Description

technical field [0001] The invention belongs to the technical field of biochemical engineering, and in particular relates to a preparation method of an apigenin molecularly imprinted polymer. Background technique [0002] Apigenin (API) is also known as apigenin, which belongs to flavonoids. It is widely found in many kinds of fruits, vegetables, beans, and tea. One of the active ingredients of Chinese herbal medicine. Apigenin has various pharmacological activities such as anti-tumor, anti-inflammation, lowering blood pressure, anti-arteriosclerosis and thrombosis, anti-anxiety, antibacterial, antiviral, and antioxidative. In recent years, the demand for high-purity apigenin has been increasing at home and abroad. At present, apigenin is mainly isolated and extracted from natural plants. Traditional methods mainly use solvent extraction for enrichment, and then separate and purify through column separation technology. Many new separation technologies have emerged recentl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F220/06C08F220/56C08F226/06C08F222/14C08F2/44C08K5/1545C08J9/26B01J20/285
Inventor 贺敏强郭利飞陈永强宋承成万金城
Owner JIANGSU UNIV
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