Preparation method of estradiol magnetic molecularly imprinted nanoparticle electrochemical sensing membrane

A magnetic molecule and molecular imprinting technology, applied in the field of analysis and detection, can solve the problems of high technical requirements for preparation and operation, limited service life, electrode wear, etc.

Inactive Publication Date: 2015-08-12
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

MIES often requires complex electrode modification and electrochemical polymerization sensing film formation process, which requires high preparation and operation technology, which is not conducive to popularization and application
Although the MIPs electropolymerization sensing film can be reused many times, the electrode polishing and regeneration process takes a long time, the electrode utilization rate is low, and the electrode wear leads to a limited service life.

Method used

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  • Preparation method of estradiol magnetic molecularly imprinted nanoparticle electrochemical sensing membrane
  • Preparation method of estradiol magnetic molecularly imprinted nanoparticle electrochemical sensing membrane
  • Preparation method of estradiol magnetic molecularly imprinted nanoparticle electrochemical sensing membrane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] 1.1 Preparation of Magnetic Molecularly Imprinted Nanoparticle Electrochemical Sensing Membrane (MMIES)

[0096] (1) Preparation of Fe 3 o 4 Au nanoparticles

[0097] Under nitrogen protection and magnetic stirring, add 180mL containing 11.2mmol FeCl to a 250mL flask 3 ·6H 2 O and 5.6 mmol FeCl 2 4H 2 The aqueous solution of O was heated to 50°C, and 12.5 mL of NH 3 ·H 2 O was reacted for 30 minutes, magnetically separated to remove the supernatant, and the reaction precipitate was collected and washed three times with water to obtain Fe 3 o 4 magnetic nanoparticles.

[0098] Take 20mgFe 3 o 4 For magnetic nanoparticles, add 10mL 1% (v / v) APTES-ethanol solution, stir and react at room temperature for 12h, remove the supernatant by magnetic separation, wash and dissolve with ethanol, and obtain APTES-coated Fe 3 o 4 nanoparticles.

[0099] Take the prepared 12.5mg APTES wrapped Fe 3 o 4 Nanoparticles, add 2.5mL 1% HAuCl4 and 35mL double distilled water, a...

Embodiment 2

[0115] 1.1 Preparation of Magnetic Molecularly Imprinted Nanoparticle Electrochemical Sensing Membrane (MMIES)

[0116] (1) Preparation of Fe 3 o 4 Au nanoparticles

[0117] Under nitrogen protection and magnetic stirring, add 200mL containing 13.1mmol FeCl to a 250mL flask 3 ·6H 2 O and 6.5 mmol FeCl 2 4H 2 The aqueous solution of O was heated to 50°C, and 12.5 mL of NH 3 ·H 2 O was reacted for 30 minutes, magnetically separated to remove the supernatant, and the reaction precipitate was collected and washed three times with water to obtain Fe 3 o 4 magnetic nanoparticles. Take 25mg Fe 3 o 4 For magnetic nanoparticles, add 15mL 1% (v / v) APTES-ethanol solution, stir and react at room temperature for 15h, remove the supernatant by magnetic separation, wash and dissolve with ethanol, and obtain APTES-coated Fe 3 o 4 nanoparticles. Take the prepared 15mg APTES wrapped Fe 3 o 4 For nanoparticles, add 4mL 1% HAuCl4 and 45mL double distilled water, ultrasonically dis...

Embodiment 3

[0133] 1.1 Preparation of Magnetic Molecularly Imprinted Nanoparticle Electrochemical Sensing Membrane (MMIES)

[0134] (1) Preparation of Fe 3 o 4 Au nanoparticles

[0135] Under nitrogen protection and magnetic stirring, add 150mL containing 10.2mmol FeCl to a 250mL flask 3 ·6H 2 O and 4.9 mmol FeCl 2 4H 2 The aqueous solution of O was heated to 50 °C, and 10 mL of NH 3 ·H 2 O was reacted for 30 minutes, magnetically separated to remove the supernatant, and the reaction precipitate was collected and washed three times with water to obtain Fe 3 o 4 magnetic nanoparticles. Take 20mgFe 3 o 4 For magnetic nanoparticles, add 8mL 1% (v / v) APTES-ethanol solution, stir and react at room temperature for 10h, remove the supernatant by magnetic separation, wash and dissolve with ethanol, and obtain APTES-coated Fe 3 o 4 nanoparticles. Take the prepared 10.5mg APTES-coated Fe 3 o 4 Nanoparticles, add 2mL 1% HAuCl4 and 30mL twice distilled water, ultrasonically disperse, ...

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Abstract

The invention discloses a preparation method of an estradiol magnetic molecularly imprinted nanoparticle electrochemical sensing membrane. According to the preparation method, an estradiol magnetic molecularly imprinted polymer is obtained via preparation of a molecularly imprinted self-assembled solution, polymerization, and template molecule elution. The estradiol magnetic molecularly imprinted nanoparticle electrochemical sensing membrane is high in selectivity, response speed, sensitivity, stability, tolerability, and universality, and is capable of realizing high efficiency, sensitive, real-time detection of estradiol in composite matrix.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and in particular relates to a preparation method of an estradiol magnetic molecule imprinted nanoparticle electrochemical sensing membrane (MMIES). Background technique [0002] 17-βestradiol (E2) is one of the endocrine disruptors. Studies have shown that children with precocious puberty, male prostate cancer, women's breast cancer and uterine cancer incidence are all related to estrogen residues in food. In addition, it will also affect the physiological laws of women, and even cause infertility. Trace levels of estradiol can cause disorders of humoral immunity and cellular immunity, resulting in reproductive immunity, cardiovascular and nervous system lesions. However, due to the residue and illegal addition of estrogen in the production process, it often causes problems such as environmental pollution and food safety. In order to ensure the health of consumers and regulate t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/333G01N27/26
Inventor 周学敏姜慧君沈心韩青徐磊段白云
Owner NANJING MEDICAL UNIV
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