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Organic arsenide molecularly imprinted membrane substrate in aptamer-based marine products, and production method and application thereof

A nucleic acid aptamer and molecularly imprinted membrane technology, applied in the field of analytical chemistry, can solve problems such as staying in the experimental stage, unable to meet the needs of molecular imprinting technology development, rare MIPs, etc. Quantitative screening, improved sensitivity and selectivity

Inactive Publication Date: 2012-06-27
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Commonly used functional monomers are only methacrylic acid, trifluoromethacrylic acid, acrylamide, N-vinylpyrrolidone, 2-vinylpyridine, 4-vinylpyridine, etc., which cannot meet the needs of the development of molecular imprinting technology; ( 3) At present, the preparation of MIPs is only in the experimental stage, and the MIPs used for actual sample detection are rare

Method used

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  • Organic arsenide molecularly imprinted membrane substrate in aptamer-based marine products, and production method and application thereof
  • Organic arsenide molecularly imprinted membrane substrate in aptamer-based marine products, and production method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 ( monomethylarsinic acid)

[0037] A method for preparing a nucleic acid aptamer-based monomethylarsinic acid molecularly imprinted membrane substrate, comprising the following steps:

[0038] (1) Select functional monomeric nucleic acid aptamers that can synthesize molecularly imprinted polymers with monomethylarsinic acid;

[0039] (2) The template molecule monomethylarsinic acid, the functional monomer nucleic acid aptamer, the cross-linking agent ethylene glycol dimethacrylate (EGDMA), the porogen chloroform, the initiator azobisisobutyronitrile, organic The solvent dichloromethane is mixed uniformly at a molar ratio of 0.1:2.5:0.1:60:0.05:2.0 to obtain a molecularly imprinted polymer solution of monomethylarsinic acid based on nucleic acid aptamers;

[0040] (3) First clean the platinum quartz crystal substrate of the piezoelectric quartz crystal microbalance with deionized water, then soak it in pure methanol for 1 hour, then rinse it with deionized w...

Embodiment 2

[0044] Example 2 (dimethylarsinic acid)

[0045] A method for preparing a nucleic acid aptamer-based dimethylarsinic acid molecularly imprinted membrane substrate, comprising the following steps:

[0046] (1) Select functional monomeric nucleic acid aptamers that can synthesize molecularly imprinted polymers with dimethylarsinic acid;

[0047] (2) The template molecule dimethylarsinic acid, the functional monomer nucleic acid aptamer, the cross-linking agent trimethylolpropane trimethacrylate, the porogen dichloromethane, the initiator azobisisobutyronitrile, The organic solvent dichloromethane is mixed uniformly at a molar ratio of 0.5:2.5:3:50:0.1:2.5 to obtain a molecularly imprinted polymer solution of dimethylarsinic acid based on nucleic acid aptamers;

[0048] (3) First clean the platinum quartz crystal substrate of the piezoelectric quartz crystal microbalance with deionized water, then soak it in pure methanol for 3 hours, then rinse it with deionized water for 5 t...

Embodiment 3

[0052] Example 3 (Methylarsinic acid)

[0053] A method for preparing a nucleic acid aptamer-based methylarsinic acid molecularly imprinted membrane substrate, comprising the following steps:

[0054] (1) Select functional monomeric nucleic acid aptamers that can synthesize molecularly imprinted polymers with methylarsinic acid;

[0055] (2) The template molecule methylarsinic acid, the functional monomer nucleic acid aptamer, the cross-linking agent N, N-methylenebisacrylamide, the porogen methanol, the initiator azobisisobutyronitrile, and the organic solvent Carbon tetrachloride is mixed uniformly at a molar ratio of 2:2.5:2.5:55:0.01:10 to obtain a molecularly imprinted polymer solution of methylarsinic acid based on nucleic acid aptamers;

[0056] (3) Clean the platinum quartz crystal substrate of the piezoelectric quartz crystal microbalance with deionized water first, then soak it in pure methanol for 2 hours, then rinse it with deionized water for 4 times, then clea...

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Abstract

The invention discloses an organic arsenide molecularly imprinted membrane substrate in aptamer-based marine products, and a production method and application thereof, wherein an organic arsenide molecularly imprinted polymer in the aptamer-based marine product is taken as a recognition element to form a reaction layer on the surface of a gold quartz body substrate; and the organic arsenide molecularly imprinted polymer in the aptamer-based marine product is formed by polymerizing organic arsenide template molecule, aptamer functional monomer, cross-linking agent, porogen, initiating agent and organic solvent in the marine product according to the molar ratio of (0.1-2.5):3:(0.1-3):(30-60):(0.01-0.15):(1.0-10). The surface finish technology is applied to the preparation of the organic arsenide molecularly imprinted membrane substrate in the aptamer-based marine product, so the preparation of the organic arsenide molecularly imprinted membrane substrate in the aptamer-based marine product has controllability, and the sensitivity and the accuracy of the substrate are improved.

Description

[0001] technical field [0002] The invention relates to a nucleic acid aptamer-based organic arsenide molecularly imprinted membrane substrate in seafood, a preparation method and application thereof, and belongs to the technical field of analytical chemistry. Background technique [0003] Arsenic contamination of seafood has always been the focus of attention in the field of food safety. Unlike terrestrial organisms, marine organisms generally have a strong ability to accumulate arsenic. Fish, shellfish, seaweed and other seafood that humans often eat contain high concentrations of arsenic. Arsenic in seafood mainly exists in the form of organic arsenic compounds (such as methylarsinic acid, methylarsinic acid, arsenoic sugar, arsenocholine, etc.), accounting for about 95% of the total arsenic. At present, the toxicity of arsenosugar and arsenocholine has not been confirmed, but methylarsinic acid has been confirmed to have strong cell and reproductive toxicity, and methy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N5/02
Inventor 刘素黄加栋颜梅于京华葛慎光邢宪荣廉文静崔敏李杰
Owner UNIV OF JINAN
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