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Nucleic aptamer-based organic arsenic molecular imprinting polymer in marine products, preparation method thereof and application thereof

A nucleic acid aptamer and molecular imprinting technology, which is used in the preparation of organic arsenic molecularly imprinted polymers in seafood. The detection sensitivity is not satisfactory, the practicability is not high, the instrument is expensive, etc., to achieve good molecular recognition performance, improve sensitivity and selectivity

Inactive Publication Date: 2013-12-18
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, chromatography technology can accurately separate organic arsenic in seafood, but the detection sensitivity is not satisfactory; mass spectrometry and coupled techniques (such as gas chromatography-mass spectrometry, liquid chromatography-mass spectrometry, etc.) The content of various organic arsenic in the product, but the instruments required by these two methods are expensive, and the sample pretreatment is extremely complicated, so the practicability is not high

Method used

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  • Nucleic aptamer-based organic arsenic molecular imprinting polymer in marine products, preparation method thereof and application thereof

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Comparison scheme
Effect test

Embodiment 1

[0024] The method of the present invention: Weigh 0.4785g functional monomer nucleic acid aptamer, 0.0255g template molecule monomethylarsonic acid, 3.2145g crosslinker trimethylolpropane trimethacrylate, 0.0650g initiator azobis Isobutyronitrile and 15ml porogen dichloromethane were mixed uniformly, put into a 25ml ampere flask, bubbling nitrogen for 10 minutes, sealed, and reacted for 48 hours under a 130w high pressure mercury lamp. The polymer monoliths are taken out, ground, extracted, dried, and sieved. Use a methanol: acetic acid (volume ratio 10:1) mixed solution to repeatedly ultrasonically clean the prepared polymer until no template molecule can be detected at the maximum absorption wavelength, then rinse with methanol to remove excess acetic acid, and dry to obtain a nucleic acid-based A molecularly imprinted polymer of monomethylarsonic acid for gametes.

[0025] Comparative test: Weigh 0.4250g functional monomer acrylic acid, 0.0255g template molecule monomethylars...

Embodiment 2

[0029] The method of the present invention: respectively weigh 0.4145g functional monomer nucleic acid aptamer, 0.0325g template molecule dimethylarsonic acid, 3.8780g crosslinking agent N,O-diacryloyl-L-amphetamine, 0.0760g initiator couple Nitrogen diisobutyronitrile, 15ml porogen dimethyl sulfoxide, mix well, put into a 25ml ampere flask, bubbling nitrogen for 10 minutes, sealing, and polymerizing under a 130w high pressure mercury lamp for 36 hours. The polymer monoliths are taken out, ground, extracted, dried, and sieved. Use acetonitrile: acetic acid volume ratio 25:1 mixture solution to ultrasonically clean the prepared polymer repeatedly until no template molecules can be detected at the maximum absorption wavelength, then wash with methanol to remove excess acetic acid, and dry to obtain nucleic acid-based aptamers Molecularly imprinted polymer of dimethylarsine acid.

[0030] Comparative test: Weigh 0.4195g functional monomer 4-vinylpyridine, 0.0325g template molecule ...

Embodiment 3

[0034] The method of the present invention: Weigh 0.3685g functional monomer nucleic acid aptamer, 0.0330g template molecule methylarsenic acid, 3.5785g crosslinker divinylbenzene, 0.0655g initiator azobisisobutyronitrile, 15ml The porogen chloroform was mixed uniformly, and filled into a 25ml ampere flask, nitrogen gas was introduced for 10 minutes, sealed, and polymerized under a 130w high pressure mercury lamp for 48 hours. The polymer monoliths are taken out, ground, extracted, dried, and sieved. Use the mixed solution of acetonitrile: acetic acid (volume ratio 15:1) to repeatedly ultrasonically clean the prepared polymer until no template molecule can be detected at the maximum absorption wavelength, then wash with methanol to remove excess acetic acid, and dry to obtain a nucleic acid-based Methylarsenic acid molecularly imprinted polymer of gametes.

[0035] Comparative test: Weigh 0.4125g functional monomer methacrylic acid, 0.0330g template molecule methylarsenic acid, ...

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Abstract

The invention discloses a nucleic aptamer-based organic arsenic molecular imprinting polymer in marine products, a preparation method thereof and application thereof. The molecular imprinting polymer is prepared by polymerizing organic arsenide template molecules, a functional monomer, a cross-linking agent, a pore-forming agent, an initiator and an organic solvent in a molar ratio of (0.1-2.5):3.0:(0.1-4):(30-60):(0.01-0.10):(1.0-10), wherein the functional monomer is a nucleic aptamer. The method for preparing the organic arsenic molecular imprinting polymer comprises the following steps of: (1) mixing the organic arsenide template molecules, the functional monomer, the cross-linking agent, the pore-forming agent, the initiator and the organic solvent uniformly in a molar ratio, and polymerizing; (2) taking the obtained polymer out of a reactor, grinding and sieving; and eluting by using eluent until the polymer does not contain bacterial toxins, cleaning by using the organic solvent until the solution is neutral, and drying under vacuum to obtain the molecular imprinting polymer. The molecular imprinting polymer has the excellent performance of molecular recognition, so that the sensitivity and selectivity of detecting organic arsenic in the marine products are improved greatly.

Description

technical field [0001] The invention relates to a seafood detection technology, in particular to a nucleic acid aptamer-based organic arsenic molecularly imprinted polymer in seafood that has the ability to specifically identify organic arsenic in seafood as a template molecule and a preparation method thereof; It also relates to the application of nucleic acid aptamers as functional monomers in the preparation of organic arsenic molecularly imprinted polymers in seafood. Background technique [0002] Arsenic contamination of seafood has always been the focus of attention in the field of food safety. Unlike terrestrial organisms, marine organisms generally have a strong ability to accumulate arsenic. Fish, shellfish, seaweed and other seafood that humans often eat contain high concentrations of arsenic. Arsenic in seafood mainly exists in the form of organic arsenic (such as methylarsinic acid, methylarsinic acid, arsenic sugar, arsenocholine, etc.), accounting for about 95...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F222/14C08F220/06C08F220/58C08F212/36C08F226/06C08F222/16C08F222/38C08J9/28C08J9/26
Inventor 刘素黄加栋颜梅于京华葛慎光邢宪荣廉文静崔敏卢兴洁李杰
Owner UNIV OF JINAN
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