Preparation method and application of pancreatic cancer immunosensor based on gold electrodeposition and Au@Ag/CuO-GS as markers
An immune sensor and pancreatic cancer technology, which is applied in the field of preparation of pancreatic cancer immunosensors, can solve the problems of low cure rate, low diagnosis rate of pancreatic cancer, and high surgical mortality, so as to improve sensitivity, reproducibility and stability , the effect of high catalytic performance
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Embodiment 1
[0038] Example 1 Preparation method and application of a pancreatic cancer immunosensor based on gold electrodeposition and AuAg / CuO-GS as markers
[0039] 1. A method for preparing a pancreatic cancer immunosensor based on gold electrodeposition and AuAg / CuO-GS as a marker, characterized in that the steps are as follows:
[0040] (1) A glassy carbon electrode with a diameter of 3 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water;
[0041] (2) HAuCl with a mass concentration of 0.8% 4 The solution is the bottom liquid, and the voltage is scanned at -0.2 V for 30 s, and gold is electrodeposited on the surface of the electrode, rinsed with ultrapure water, and dried in the air;
[0042] (3) Continue to add 4 μL, 8 μg / mL tumor marker capture antibody Ab 1 The solution was added dropwise to the surface of the electrode, rinsed with ultrapure water, and dried in a refrigerator at 4 °C;
[0043] (4) Continue to add 3 μL of bovine serum al...
Embodiment 2
[0046] Example 2 Preparation method and application of a pancreatic cancer immunosensor based on gold electrodeposition and AuAg / CuO-GS as markers
[0047] (1) Glassy carbon electrodes with a diameter of 3-5 mm are covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water;
[0048] (2) HAuCl with a mass concentration of 1.2% 4 The solution is the bottom liquid, and the voltage is scanned at -0.2 V for 30 s, and gold is electrodeposited on the surface of the electrode, rinsed with ultrapure water, and dried in the air;
[0049] (3) Continue to add 5 μL, 10 μg / mL tumor marker capture antibody Ab 1 The solution was added dropwise to the surface of the electrode, rinsed with ultrapure water, and dried in a refrigerator at 4 °C;
[0050] (4) Continue to add 3 μL of bovine serum albumin BSA solution with a mass fraction of 1.2% dropwise to the electrode surface to seal the non-specific active sites on the electrode surface, rinse with ultrapure water, a...
Embodiment 3
[0053] Example 3 Preparation method and application of a pancreatic cancer immunosensor based on gold electrodeposition and AuAg / CuO-GS as markers
[0054] (1) Glassy carbon electrodes with a diameter of 3-5 mm are covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water;
[0055] (2) HAuCl with a mass concentration of 1.5% 4 The solution is the bottom liquid, and the voltage is scanned at -0.2 V for 30 s, and gold is electrodeposited on the surface of the electrode, rinsed with ultrapure water, and dried in the air;
[0056] (3) Continue to add 6 μL, 12 μg / mL tumor marker capture antibody Ab 1 The solution was added dropwise to the surface of the electrode, rinsed with ultrapure water, and dried in a refrigerator at 4 °C;
[0057] (4) Continue to drop 3 μL of 2.0% bovine serum albumin BSA solution onto the electrode surface to seal the non-specific active sites on the electrode surface, rinse with ultrapure water, and dry in a refrigerator at 4 ...
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