Preparation method and application of pancreatic cancer immunosensor based on gold electrodeposition and Au@Ag/CuO-GS as markers

An immune sensor and pancreatic cancer technology, which is applied in the field of preparation of pancreatic cancer immunosensors, can solve the problems of low cure rate, low diagnosis rate of pancreatic cancer, and high surgical mortality, so as to improve sensitivity, reproducibility and stability , the effect of high catalytic performance

Active Publication Date: 2015-03-25
UNIV OF JINAN
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

The early diagnosis rate of pancreatic cancer is not high, the surgical mortality rate is high, and the cure rate is very low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Preparation method and application of a pancreatic cancer immunosensor based on gold electrodeposition and AuAg / CuO-GS as markers

[0039] 1. A method for preparing a pancreatic cancer immunosensor based on gold electrodeposition and AuAg / CuO-GS as a marker, characterized in that the steps are as follows:

[0040] (1) A glassy carbon electrode with a diameter of 3 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water;

[0041] (2) HAuCl with a mass concentration of 0.8% 4 The solution is the bottom liquid, and the voltage is scanned at -0.2 V for 30 s, and gold is electrodeposited on the surface of the electrode, rinsed with ultrapure water, and dried in the air;

[0042] (3) Continue to add 4 μL, 8 μg / mL tumor marker capture antibody Ab 1 The solution was added dropwise to the surface of the electrode, rinsed with ultrapure water, and dried in a refrigerator at 4 °C;

[0043] (4) Continue to add 3 μL of bovine serum al...

Embodiment 2

[0046] Example 2 Preparation method and application of a pancreatic cancer immunosensor based on gold electrodeposition and AuAg / CuO-GS as markers

[0047] (1) Glassy carbon electrodes with a diameter of 3-5 mm are covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water;

[0048] (2) HAuCl with a mass concentration of 1.2% 4 The solution is the bottom liquid, and the voltage is scanned at -0.2 V for 30 s, and gold is electrodeposited on the surface of the electrode, rinsed with ultrapure water, and dried in the air;

[0049] (3) Continue to add 5 μL, 10 μg / mL tumor marker capture antibody Ab 1 The solution was added dropwise to the surface of the electrode, rinsed with ultrapure water, and dried in a refrigerator at 4 °C;

[0050] (4) Continue to add 3 μL of bovine serum albumin BSA solution with a mass fraction of 1.2% dropwise to the electrode surface to seal the non-specific active sites on the electrode surface, rinse with ultrapure water, a...

Embodiment 3

[0053] Example 3 Preparation method and application of a pancreatic cancer immunosensor based on gold electrodeposition and AuAg / CuO-GS as markers

[0054] (1) Glassy carbon electrodes with a diameter of 3-5 mm are covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water;

[0055] (2) HAuCl with a mass concentration of 1.5% 4 The solution is the bottom liquid, and the voltage is scanned at -0.2 V for 30 s, and gold is electrodeposited on the surface of the electrode, rinsed with ultrapure water, and dried in the air;

[0056] (3) Continue to add 6 μL, 12 μg / mL tumor marker capture antibody Ab 1 The solution was added dropwise to the surface of the electrode, rinsed with ultrapure water, and dried in a refrigerator at 4 °C;

[0057] (4) Continue to drop 3 μL of 2.0% bovine serum albumin BSA solution onto the electrode surface to seal the non-specific active sites on the electrode surface, rinse with ultrapure water, and dry in a refrigerator at 4 ...

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Abstract

The invention relates to a preparation method and application of a pancreatic cancer immunosensor based on gold electrodeposition and Au@Ag / CuO-GS as markers, and particularly relates to preparation of a sandwich-type electrochemical immunosensor for detecting pancreatic cancer tumor markers by adopting Au@Ag / CuO-GS as a detection antibody marker. By using excellent biocompatibility and high catalytic performance of the Au@Ag / CuO-GS, the prepared sensor has relatively high sensitivity and relatively wide detection range; and the detection limit can reach 1.5fg / mL.

Description

technical field [0001] The invention relates to a preparation method and application of a pancreatic cancer immunosensor based on gold electrodeposition and AuAg / CuO-GS as markers. Specifically, AuAg / CuO-GS is used as a detection antibody marker to prepare a sandwich-type electrochemical immunosensor for detection of pancreatic cancer tumor markers, which belongs to the technical field of new functional materials and biosensing detection. Background technique [0002] Pancreatic cancer is a highly malignant tumor of the digestive tract that is difficult to diagnose and treat. Its morbidity and mortality have increased significantly in recent years. The 5-year survival rate is <1%, and it is one of the malignant tumors with the worst prognosis. The early diagnosis rate of pancreatic cancer is not high, the surgical mortality rate is high, and the cure rate is very low. In clinical research, it is very important to develop a fast, simple and sensitive method for detectin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N27/416G01N33/532
CPCG01N27/416G01N33/532G01N33/57438
Inventor 吴丹闫涛李月云孙培晓王晓东庞雪辉马洪敏张勇范大伟杜斌胡丽华魏琴
Owner UNIV OF JINAN
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