Preparation method and application of a PD/V2O5/mwcnts-based immunosensor for colorectal cancer tumor markers

A technology of tumor markers and immune sensors, which is applied in the direction of instruments, scientific instruments, measuring devices, etc., can solve the problems of cumbersome detection methods, complicated operations, and high costs, and achieve the effects of fast detection speed, simple operation, and avoiding detection errors

Inactive Publication Date: 2016-06-29
SHANDONG UNIV OF TECH
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are many clinical detection methods for cancer markers, such as radioimmunoassay, immunoradiometric assay, enzyme-labeled immunoassay, chemiluminescence assay, time-resolved fluorescence immunoassay, etc., but most of the detection methods are cumbersome, The operation is complicated, the cost is expensive, and the detection limit is high. Therefore, it is of great significance to establish a fast, simple and sensitive detection method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1. A kind of based on Pd / V 2 o 5 The preparation method of the intestinal cancer tumor marker immunosensor of MWCNTs is characterized in that, the steps are as follows:

[0046] (1) A glassy carbon electrode with a diameter of 3mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water;

[0047] (2) Drop-coat 6 μL, 1.0 mg / mL tin dioxide-doped graphene solution on the surface of the electrode, dry it, rinse it with ultrapure water, and dry it;

[0048] (3) Drop-coat 6 μL of gold nanoparticle solution on the surface of the electrode, let it dry, rinse it with ultrapure water, and let it dry;

[0049] (4) Drop-coat 6 μL, 8 μg / mL colorectal cancer tumor marker capture antibody Ab 1 solution on the electrode surface, 4 o C dry in refrigerator;

[0050] (5) Rinse away unbound capture antibody Ab with ultrapure water 1 After that, drop 6 μL of bovine serum albumin BSA solution with a mass fraction of 0.1% on the surface of the ...

Embodiment 2

[0053] Embodiment 2. A kind of based on Pd / V 2 o 5 The preparation method of the intestinal cancer tumor marker immunosensor of MWCNTs is characterized in that, the steps are as follows:

[0054] (1) A glassy carbon electrode with a diameter of 4 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water;

[0055] (2) Drop-coat 6 μL, 1.5 mg / mL tin dioxide-doped graphene solution on the surface of the electrode, let it dry, rinse it with ultrapure water, and let it dry;

[0056] (3) Drop-coat 6 μL of gold nanoparticle solution on the surface of the electrode, let it dry, rinse it with ultrapure water, and let it dry;

[0057] (4) Drop-coat 6 μL, 10 μg / mL colorectal cancer tumor marker capture antibody Ab 1 solution on the electrode surface, 4 o C dry in refrigerator;

[0058] (5) Rinse away unbound capture antibody Ab with ultrapure water 1 After that, drop 6 μL of bovine serum albumin BSA solution with a mass fraction of 0.5% on the surfa...

Embodiment 3

[0061] Embodiment 3. A kind of based on Pd / V 2 o 5 The preparation method of the intestinal cancer tumor marker immunosensor of MWCNTs is characterized in that, the steps are as follows:

[0062] (1) A glassy carbon electrode with a diameter of 5mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water;

[0063] (2) Drop-coat 6 μL, 2.0 mg / mL tin dioxide-doped graphene solution on the surface of the electrode, dry it, rinse it with ultrapure water, and dry it;

[0064] (3) Drop-coat 6 μL of gold nanoparticle solution on the surface of the electrode, let it dry, rinse it with ultrapure water, and let it dry;

[0065] (4) Drop-coat 6 μL, 12 μg / mL colorectal cancer tumor marker capture antibody Ab 1 solution on the electrode surface, 4 o C dry in refrigerator;

[0066] (5) Rinse away unbound capture antibody Ab with ultrapure water 1 After that, drop 6 μL of bovine serum albumin BSA solution with a mass fraction of 1.0% on the surface of the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to view more

Abstract

The invention belongs to the technical fields of nanometer functional materials, immune analysis and biosensing, and provides a preparation method and application of a Pd / V2O5 / MWCNTs-based intestinal cancer tumor marker immunosensor. Specifically, Pd / V2O5 / MWCNTs was used as a detection antibody marker to prepare a sandwich-type electrochemical immunosensor for the detection of intestinal cancer tumor markers. By utilizing the excellent biocompatibility and high catalytic performance of Pd / V2O5 / MWCNTs, The fabricated sensor has the advantages of strong specificity, high sensitivity and low detection limit.

Description

technical field [0001] The present invention relates to a kind of based on Pd / V 2 o 5 Preparation method and application of intestinal cancer tumor marker immunosensor of MWCNTs. Specifically using Pd / V 2 o 5 / MWCNTs is used as a detection antibody marker to prepare a sandwich-type electrochemical immunosensor for the detection of intestinal cancer tumor markers, which belongs to the technical fields of new functional materials, immune analysis and biosensing detection. Background technique [0002] Colon cancer is a common malignant tumor in the gastrointestinal tract, and its morbidity and mortality have increased significantly in recent years. Early diagnosis of intestinal cancer can greatly increase the cure rate and improve the quality of life of patients. In clinical research, it is very important to develop a fast, simple and sensitive method for detecting tumor markers of intestinal cancer. [0003] At present, there are many clinical detection methods for cance...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574G01N33/543
CPCG01N33/54346G01N33/5438G01N33/57419
Inventor 李月云王平韩健董云会刘青陈磊
Owner SHANDONG UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap