A preparation method and application of a methyl parathion immunosensor based on the combination of two electrochemical methods

A methyl parathion, immunosensor technology, applied in the direction of electrochemical variables of materials, nanotechnology for materials and surface science, analysis by chemical reactions of materials, etc., can solve the limitation of wide application, affect operation The convenience and other issues of high-selectivity detection, convenient operation, and broad market development prospects are achieved.

Inactive Publication Date: 2018-05-08
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, because electrochemiluminescence sensors require external light signal capture devices such as photodiodes, while photoelectrochemical sensors require external light sources to excite photosensitive materials, this affects the convenience of both operations to a certain extent and limits They are more widely used in actual production and life

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 A methylparathion immunosensor based on the combination of two electrochemical methods

[0037] A method for preparing a methyl parathion immunosensor based on the combination of two electrochemical methods, the specific preparation steps are:

[0038] (1) Cadmium-doped molybdenum disulfide two-dimensional nanomaterial Cd-MoS 2 Solution preparation:

[0039] The cadmium-doped molybdenum disulfide two-dimensional nanomaterial Cd-MoS 2 The solution is Cd-MoS 2 aqueous solution, the Cd-MoS 2 It is a molybdenum disulfide two-dimensional nanomaterial doped with cadmium ions. The two-dimensional nanomaterial is in the form of a paper sheet with a thickness of about 10-20 nm. The Cd-MoS 2 The preparation method is: 0.005 g cadmium chloride CdCl 2 , 2 mL of 0.1 mol / L ascorbic acid solution, 0.5 mL of sodium molybdate Na 2 MoO 4 solution and 0.01 g sodium sulfide Na 2 S, after mixing and stirring for 15 minutes, put it into the reaction kettle, and react for 16...

Embodiment 2

[0057] Example 2 A methyl parathion immunosensor based on the combination of two electrochemical methods

[0058] A method for preparing a methyl parathion immunosensor based on the combination of two electrochemical methods, the specific preparation steps are:

[0059] (1) Cd-MoS 2 Solution preparation:

[0060] The preparation steps are the same as that of Cd-MoS in Example 1 2 The method of preparation of the solution differs in: CdCl 2 The addition amount of ascorbic acid solution is 0.008 g, the addition amount of ascorbic acid solution is 4 mL, Na 2 MoO 4 The amount of solution added was 1.0 mL and Na 2 The amount of S added was 0.02 g, and the reaction was carried out at 220° C. for 12 hours.

[0061] (2) Preparation of electrochemiluminescence working electrode W1:

[0062] The preparation steps are the same as those of W1 in Example 1, except that the Cd-MoS prepared in (1) in this example is used 2 Modified electrodes; the concentration of luminol in the elec...

Embodiment 3

[0070] Example 3 A methylparathion immunosensor based on the combination of two electrochemical methods

[0071] A method for preparing a methyl parathion immunosensor based on the combination of two electrochemical methods, the specific preparation steps are:

[0072] (1) Cd-MoS 2 Solution preparation:

[0073] The preparation steps are the same as that of Cd-MoS in Example 1 2 The method of preparation of the solution differs in: CdCl 2 The addition amount of ascorbic acid solution is 0.01 g, the addition amount of ascorbic acid solution is 6 mL, Na 2 MoO 4 The amount of solution added was 1.5 mL and Na 2 The amount of S added was 0.03 g, and the reaction was carried out at 220° C. for 12 hours.

[0074] (2) Preparation of electrochemiluminescent working electrode W1:

[0075] The preparation steps are the same as those of W1 in Example 1, except that the Cd-MoS prepared in (1) in this example is used 2 Modified electrodes; the concentration of luminol in the electro...

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Abstract

The invention discloses a preparation method of a bioimmune sensor with dual signal development strategies of electrochemiluminescence and photoelectrochemistry constructed on the basis of functional nanomaterials. The prepared sensor is simple to operate, easy to carry, fast to detect, and low in cost, and can be used in Rapid and sensitive detection of parathion in daily production and daily life.

Description

technical field [0001] The invention relates to a preparation method and application of an immunosensor for detecting methyl parathion, and belongs to the technical field of novel nanometer functional materials and electrochemical biosensing analysis. Background technique [0002] Methyl parathion is a broad-spectrum highly toxic insecticide, which has contact killing, stomach poisoning and fumigation effects, and can penetrate into plants. Methyl parathion has a fast action on insects, and can be used to control cotton, apple, citrus, pear, peach and other fruit tree pests and wheat red spiders. In soil, methyl parathion can slightly migrate to the deep soil layer through water leaching. Methyl parathion in the soil can enter the plant body through the absorption of plant roots. After people accidentally eat such plants or plants containing their residues, methyl parathion can enter the human body through the digestive tract, respiratory tract and intact skin and mucous m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/48G01N21/76B82Y30/00
CPCB82Y30/00G01N21/76G01N27/48
Inventor 张勇邓保军庞雪辉任祥魏琴
Owner UNIV OF JINAN
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