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Preparation method and application of estradiol photoelectrochemical sensor based on self-illumination excitation

A self-luminous excitation, photoelectrochemical technology, applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, electrochemical variables of materials, etc., can solve problems such as limiting wide application and affecting the convenience of operation, Achieve high selectivity detection, easy operation, improve detection sensitivity and detection limit effect

Inactive Publication Date: 2016-07-27
UNIV OF JINAN
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, since electrochemiluminescence sensors require external light signal capture devices such as photodiodes, while photoelectrochemical sensors require external light sources to excite photosensitive materials, this affects the convenience of both operations to a certain extent and limits They are more widely used in actual production and life

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1 A kind of estradiol photoelectrochemical sensor based on self-luminous excitation

[0038] A method for preparing an estradiol photoelectrochemical sensor based on self-luminescence excitation, the specific preparation steps are:

[0039] (1) Preparation of electrochemiluminescence working electrode W1:

[0040] 1) Use ITO conductive glass as the working electrode, and drop-coat NH on the surface of the electrode 4 NiPO 4 , covering an area of ​​1cm×1cm, and dry at room temperature;

[0041] 2) Immerse the working electrode obtained in 1) into the electrolyte solution, and the immersion area is NH 4 NiPO 4 For the covered area, use a three-electrode system to electrochemically deposit the working electrode, take out the working electrode after deposition, wash it with ultrapure water, and dry it at 4°C in the dark to prepare the electrochemiluminescence working electrode W1;

[0042] The NH 4 NiPO 4 The solution is an aqueous solution of nickel ammon...

Embodiment 2

[0059] Embodiment 2 A kind of estradiol photoelectrochemical sensor based on self-luminous excitation

[0060] A method for preparing an estradiol photoelectrochemical sensor based on self-luminescence excitation, the specific preparation steps are:

[0061] (1) Preparation of electrochemiluminescent working electrode W1:

[0062] The preparation steps are the same as the preparation steps of W1 in Example 1, the difference is: the ammonium salt addition is 0.3g in the preparation step of the nickel ammonium phosphate micro-nano material, the phosphate addition is 0.20g, and the nickel dichloride addition is 0.20g, the stirring temperature and time are 38°C and 12h respectively; the concentration of luminol in the electrolyte is 5mmol / L, and the concentration of sulfuric acid is 0.5mol / L; when electrochemical deposition is carried out by cyclic voltammetry, cycle 25 times.

[0063] (2) Preparation of photoelectrochemical working electrode W2:

[0064] 1) Using ITO conducti...

Embodiment 3

[0070] Embodiment 3 A kind of estradiol photoelectrochemical sensor based on self-luminous excitation

[0071] A method for preparing an estradiol photoelectrochemical sensor based on self-luminescence excitation, the specific preparation steps are:

[0072] (1) Preparation of electrochemiluminescent working electrode W1:

[0073] The preparation steps are the same as the preparation steps of W1 in Example 1, the difference is: the ammonium salt addition is 0.4g in the preparation step of the nickel ammonium phosphate micro-nano material, the phosphate addition is 0.25g, and the nickel dichloride addition is 0.25g, the stirring temperature and time are 45°C and 14h respectively; the concentration of luminol in the electrolyte is 10mmol / L, and the concentration of sulfuric acid is 1.0mol / L; when electrochemical deposition is carried out by cyclic voltammetry, cycle 30 times.

[0074] (2) Preparation of photoelectrochemical working electrode W2:

[0075] 1) Using ITO conduct...

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Abstract

The invention discloses a preparation method of a biological immunity sensor which is based on functional nano-material construction and has dual signal development strategies of electrogenerated chemiluminescence and photoelectrochemistry, and the prepared sensor is easy to operate, convenient to carry, fast in detection, low in cost and capable of being used for fast and sensitive detection of estradiol in the fields of routine production and life and the like.

Description

technical field [0001] The invention relates to a preparation method of a sensor for detecting estradiol, and belongs to the technical field of novel nanometer functional materials and electrochemical biosensing analysis. Background technique [0002] Environmental estrogen refers to a class of compounds that interfere with the synthesis, release, transport, binding, and metabolism of normal endocrine substances in the body after entering the body, activate or inhibit the function of the endocrine system, and thereby destroy the stability and regulation of the body. There are many kinds of environmental estrogens, including synthetic compounds and plant natural estrogens, which are widely distributed in nature. Estradiol is a synthetic estrogen that is a steroid. At present, the methods for detecting estradiol mainly include chromatography and mass spectrometry. Such methods require expensive instruments and complex operations, and laboratory personnel need professional tr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N21/76G01N27/30G01N27/327
CPCG01N21/76G01N27/305G01N27/327G01N33/54306G01N33/54386
Inventor 张勇邓保军马洪敏王耀光魏琴
Owner UNIV OF JINAN
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