15 results about "Estradiolum" patented technology

The invention discloses a traditional Chinese medicine composition, a preparation method thereof and an application of the traditional Chinese medicine composition in preparation of a medicine for preventing and treating ovarian reserve hypofunction, and belongs to the technical field of traditional Chinese medicines. The composition is prepared from the following raw materials in parts by weight: 10 to 15 parts of radix paeoniae alba, 6 to 10 parts of radix bupleuri, 10 to 15 parts of radix rehmanniae preparata, 15 to 30 parts of stir-fried rhizoma dioscoreae, 12 to 20 parts of semen cuscutae, 10 to 12 parts of radix angelicae sinensis, 10 to 15 parts of poria cocos, 12 to 25 parts of fructus ligustri lucidi, 10 to 15 parts of radix salviae miltiorrhizae, 10 to 15 parts of fructus corni and 10 to 15 parts of radix morindae officinalis. The traditional Chinese medicine composition can obviously reduce the level of follicle-stimulating hormone (FSH) of cis-platinum model mice, increase estradiol (E2), increase the number of follicles at all levels, especially the number of primordial follicles, and reduce the number of atresia follicles; the ovarian function of naturally aged mice can be improved, FSH (follicle stimulating hormone) is reduced, anti-mullerian hormone (AMH) and E2 are increased, and the quantity of follicles at all levels, especially the quantity of corpus luteum, primordial follicles and anterior sinus follicles, is increased; therefore, the composition disclosed by the invention has an obvious protection effect on the hypoovarian reserve function.

The invention provides a strain of lactobacillus plantarum YS-AG23 with high antioxidant activity, and a rhizoma polygonati and radix angelicae sinensis fermented product obtained by fermenting rhizoma polygonati and radix angelicae sinensis with the strain of lactobacillus plantarum YS-AG23. In a D-galactose induced premature ovarian failure mouse model, the fermentation product can regulate up the levels of antioxidant and stress protection genes SOD2 and HO-1 and an anti-aging gene SIRT1 by regulating down the expression of a key gene AGER of an inflammation and oxidative stress pathway, so that the oxidative stress and inflammatory response of model mouse ovarian tissues are remarkably reduced; meanwhile, the serum hormone level can be specifically adjusted, the serum estradiol (E2) level can be increased, the follicle stimulating hormone (FSH) and luteinizing hormone (LH) levels can be reduced to be close to the normal range, the expression of anti-mullerian hormone (AMH) and hormone regulatory gene LHR of ovarian reserve markers can be up-regulated, the proliferative activity and estrogen secretion function of follicular granulosa cells can be improved, and the effect of preventing ovarian reserve is achieved. The wet weight of atrophic uterus and ovary of the model mouse is remarkably increased, uterine and ovary atrophy is effectively reversed, and the ovarian reserve function of the D-galactose induced premature ovarian failure model mouse is recovered. Besides, in an H2O2-induced human dermal fibroblast aging model, the yeast can improve the activity of aging cells in a concentration-dependent manner and reduce the level of reactive oxygen species (ROS) in the cells; in addition, by up-regulating the expression of a collagen synthesis key gene COL1A1 and down-regulating the expression of a cell senescence marker gene p16, skin cell senescence caused by oxidative stress can be effectively improved. In conclusion, the polygonatum sibiricum and angelica sinensis leavening can synchronously improve premature ovarian failure and skin cell senescence caused by oxidative stress by accurately regulating and controlling oxidative stress, inflammatory response and tissue function related genes, and has wide application potential.

Treatment of hirsutism, polycystic ovary syndrome, and other related conditions. This invention relates to a novel treatment for hirsutism, wherein a combination of dinogest and ethinylestradiol is administered to the subject in need.

The invention belongs to the technical field of liquid crystal laser sensing, and relates to an estradiol detection device based on a liquid crystal-gold nanoparticle laser sensor. The optical flow control optical fiber is used as an echo wall resonator. In order to realize an estradiol sensing function, the DNA aptamer is modified on the inner surface of the optical flow control optical fiber to realize specific capture of estradiol. After the aptamer is combined with estradiol, the interaction between the aptamer and estradiol triggers the aptamer imaging change, so that the liquid crystal molecule realizes the conversion from a bipolar type to a radial type, and the conversion leads to the reduction of the refractive index, thereby causing the blue shift of the spectrum. By virtue of a local disturbance effect based on the gold nanoparticles, a quadruple synergistic effect of hyperpolarizability change of modified biomolecules, liquid crystal molecule orientation and echo wall mode resonance, an opportunity is provided for sensing and amplifying subtle detection of a target binding event. The problem of low sensitivity caused by common naked eye observation or wide spectrum monitoring in a liquid crystal microdroplet sensor is solved.

The invention provides application of 2-methoxyestradiol in preparation of products for preventing and treating heart failure, and belongs to the technical field of medicines. The invention provides the effect of 2-methoxyestradiol (2ME) for preventing and treating heart failure for the first time. According to the invention, an isoproterenol induced myocardial fibroblast proliferation and migration model and an in-vivo heart failure mouse model are constructed in vitro; according to the present invention, the cardiac muscle fibroblast migration rate, the cardiac function, the serum heart failure marker level, the cardiac muscle tissue collagen deposition key protein, the pathological analysis and the heart electrophysiological signal change on the heart failure mouse are detected, the anti-heart failure effect of the 2ME is researched, and the results show that the 2ME has significant treatment effect on the heart failure disease, and is superior to the positive control drug captopril;

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to application of a Dan'e Fukang preparation in preparation of a medicine for treating premature ovarian failure. The invention provides application of a Dan'e Fukang preparation in preparation of a medicine for treating premature ovarian failure. Experiments prove that the Dan'e Fukang preparation can improve uterus and ovarian organ indexes of premature ovarian failure mice; the contents of estradiol and progesterone in the serum of the premature ovarian failure mouse are improved. The Dan'e Fukang preparation can reduce the FSH level and the LH level in the serum of a premature ovarian failure rat, and can significantly increase the E2 level of the rat; the Dan'e Fukang preparation disclosed by the invention can be used for inhibiting premature ovarian failure and promoting ovarian function recovery.

This invention provides a Bacillus licheniformis strain that degrades estradiol, a bacterial agent, and its applications, belonging to the field of microbiology and environmental protection technology. This invention screened and isolated a Bacillus licheniformis strain HMF11, which possesses the function of degrading estradiol in water at low temperatures (CGMCC No. 30477). This strain exhibits strong adaptability and maintains good activity even at low temperatures, retaining its estradiol-degrading function and effectively remediating environments, especially water bodies, contaminated by estradiol. Compared to other strains, the bacterial agent product prepared using Bacillus licheniformis HMF11 can effectively remediate water environments contaminated by estradiol in early spring or late autumn in northern regions.

The invention discloses a positive ion saliva metabolite marker related to meningioma, a product and application of the positive ion saliva metabolite marker, and relates to the field of biological medicine. The positive ion saliva metabolite marker is prepared from one or more of 1-palmitoyl-2-glutaryl phosphatidylcholine, 2, 3, 5-triiodobenzene alcohol, beta-estradiol, cortisol, N-acetylcadaverine, prilocaine, octadecanoic acid, sulfamethoxam and rimsulfuron. The invention provides a kit which is used for detecting positive ion saliva metabolite markers related to meningioma and comprises a specific detection reagent. In addition, the invention also provides a computer program product which is specially used for evaluating meningioma. The products have good feasibility and accuracy, the risk of meningioma can be effectively evaluated, and a brand new powerful tool is provided for clinical diagnosis.

The invention provides bacillus licheniformis for degrading estradiol, a fungicide and application, and belongs to the technical field of microorganisms and environmental protection. According to the invention, a bacillus licheniformis HMF11 strain with a function of degrading estradiol in a water body at a low temperature is obtained through screening and separation, and the preservation number is CGMCC No.30477. The strain is strong in adaptive capacity, can keep good activity in a relatively low temperature environment, still has the function of degrading estradiol, and can effectively repair an environment, especially a water body, polluted by estradiol. Compared with other strains, a microbial agent product prepared by applying the bacillus licheniformis HMF11 can be used for effectively repairing a water environment polluted by estradiol in early spring or late autumn in northern areas.

The invention relates to the field of pharmacy, and discloses a triboelectricity-assisted micro-channel electroporation estradiol transdermal patch as well as a preparation method and application thereof. The transdermal patch comprises a skin fitting layer, a micro-channel electroporation layer, a medicine storage layer, a triboelectric layer and a backing layer, the medicine storage layer is prepared from estradiol, choline vanillin ionic liquid, estradiol-loaded ZIF-8 nanoparticles and a transdermal penetration enhancer; a micro-channel array and an interdigital electrode are arranged in the micro-channel electroporation layer; the friction electricity generation layer comprises a silver-plated nylon fabric layer, an elastic silica gel column layer, a polytetrafluoroethylene film and a polyurethane film. According to the transdermal patch disclosed by the invention, through a multi-layer structural design, on one hand, the problem of insolubility of estradiol is solved by utilizing the choline aromatic folic acid ionic liquid and the ZIF-8 nanoparticles; on the other hand, the triboelectricity structure and the micro-channel electroporation technology are ingeniously integrated, the flow direction of the medicine is guided through the micro-channel, the triboelectricity provides electroporation energy, and efficient transdermal drug delivery under the self-powered condition is achieved.

The invention belongs to the field of biomedicine, and particularly belongs to the crossing field of biomedical engineering and regenerative medicine. The technical problem to be solved by the invention is to provide an effective means for functional repair of premature ovarian failure or premature ovarian insufficiency. The technical means for solving the technical problem is to provide a method for preparing ovarian granular cell precursor cells and extracellular vesicles. The method comprises the following steps: culturing mesenchymal stem cells in the presence of estradiol, follicle stimulating hormone and transforming growth factor beta so as to differentiate the mesenchymal stem cells into ovarian granular cell precursor cells; wherein the precursor cells of the ovarian granulosa cells express granulosa cell markers FOXL2 and AMHR2. The extracellular vesicles obtained by culturing and collecting the precursor cells of the ovarian granular cells obtained by the method can achieve a better ovarian function repairing effect, so that a new means is provided for treating ovarian insufficiency diseases such as premature ovarian failure and the like.

The invention relates to the field of biology, and provides a culture medium for cervical stomach type adenocarcinoma organs and application of the culture medium, the culture medium comprises a basic culture medium and an additive, and the additive comprises Neuregulin 1 and at least one of the following components: B27, Y-27632, estradiol, A83-01, EGF, Noggin, R-Spondin1, hydrocortisone, Forskolin and wnt3a. According to the present invention, with the organoid culture medium, the cervical stomach type adenocarcinoma organoid can be effectively obtained, and the cervical stomach type adenocarcinoma organoid can maintain the histomorphological characteristics of the original tumor tissue, can rapidly amplify and grow, and can be stably subjected to passage cryopreservation and resuscitation, such that the important research platform is provided for the personalized drug screening and treatment, and the application prospect is broad. The method has profound significance for scientific research and clinical application.

An estrosphere preparation comprising an ester phase and an aqueous phase characterised in that the ester phase comprises α-linolenic acid ethyl ester, or a mixture of α-linolenic acid ethyl ester with one or more components selected from the group comprising polyunsaturated fatty acids ethyl esters (PUFA ethyl esters) or triglycerides, ethanol, and emulsifier, in a weight ratio of between 2:0.5:1 and 8:2:1, respectively, and the aqueous phase comprises water with glycerol at a final concentration of 2.25% by weight, wherein the weight ratio of α-linolenic acid ethyl ester, or of the mixture of α-linolenic acid ethyl ester with one or more components to ethanol, emulsifier and water is between 2:0.5:1:45 and 8:2:1:45, respectively. A method for producing such an estrosphere preparation. Said preparation for use in the treatment of cancer and viral diseases.

The invention discloses an induction method of dopaminergic neural stem cells, and relates to the technical field of cell culture and neurobiology, and the induction method comprises the following steps: inoculating neural stem cells into an induction culture medium, culturing under the conditions of 37 DEG C and 5% CO2, and carrying out induced differentiation for 6-14 days to obtain dopaminergic neurons; the induction culture medium takes DMEM / F12 as a basic culture medium, and contains an effective amount of the following components: human insulin, progesterone, hydrocortisone, dexamethasone, estradiol, transferrin, an epidermal growth factor (EGF) and a fibroblast growth factor (FGF). The induction culture medium and the method have the advantages that the survival of neural stem cells can be effectively supported; and the gene is guided to directionally differentiate towards the dopaminergic neuron lineage. The method is clear in operation flow and stable in differentiation process, the expression of the characteristic marker of the dopaminergic neuron can be detected in the obtained cell population, and a stable and reliable cell source is provided for related scientific research.

FIELD: virology; neurology.SUBSTANCE: method for treating rabies in humans after symptoms appear has been proposed. The method includes administering to the patient an estradiol-type compound, anti-rabies immunoglobulin, and the preparation Imunofan, performing therapeutic hypothermia, and placing the patient into a therapeutic coma with artificial ventilation of the patient's lungs.EFFECT: increase in the survival rate of rabies patients after the onset of symptoms.3 cl, 1 tbl