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Preparation method and application of a dimeform-free enzyme sensor based on molybdenum sulfide composite material

A composite material, enzyme sensor technology, applied in the field of new nano functional materials and electrochemical biosensing analysis, can solve the problems affecting the convenience of operation, limit wide application, etc., achieve convenient operation, high selectivity detection, preparation simple effect

Inactive Publication Date: 2018-09-14
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, since the electrochemiluminescence sensor requires external light signal capture devices such as photodiodes, and the photoelectrochemical sensor requires an external light source to excite the photosensitive material, this affects the convenience of the operation of the two to a certain extent and limits them. Wider application in actual production and life

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 A chlordimeform enzyme-free sensor based on molybdenum sulfide composite material, the specific preparation steps are:

[0038] (1) Zn-MoS 2 Preparation of:

[0039] 0.005 g zinc chloride ZnCl 2 , 2 mL of 0.1 mol / L ascorbic acid solution, 0.5 mL of sodium molybdate Na 2 MoO 4 solution and 0.01 g sodium sulfide Na 2 S, after mixing and stirring for 15 minutes, put it into the reaction kettle, and react for 16 hours at 150°C; after cooling to room temperature, use deionized water to wash centrifugally, and vacuum-dry at 40°C to obtain Zn-MoS 2 , which was dissolved in deionized water to obtain Zn-MoS 2 solution.

[0040] (2) Preparation of ECL working electrode W1:

[0041] 1) Using ITO conductive glass as the working electrode, drop-coat the Zn-MoS prepared in (1) on the surface of the electrode 2 solution, covering an area of ​​1 cm × 1 cm, and allowed to dry at room temperature;

[0042] 2) Immerse the working electrode obtained in 1) into the electr...

Embodiment 2

[0057] Example 2 A chlordimeform enzyme-free sensor based on a molybdenum sulfide composite material, the specific preparation steps are:

[0058] (1) Zn-MoS 2 Preparation of:

[0059] The preparation steps are the same as that of Zn-MoS in Example 1 2 The method of preparation of the solution differs in: ZnCl 2 The addition amount of ascorbic acid solution is 0.008 g, the addition amount of ascorbic acid solution is 4 mL, Na 2 MoO 4 The amount of solution added was 1.0 mL and Na 2 The amount of S added was 0.02 g, and the reaction was carried out at 220° C. for 12 hours.

[0060] (2) Preparation of ECL working electrode W1:

[0061] The preparation steps are the same as those of W1 in Example 1, except that the Zn-MoS prepared in (1) in this example is used 2 Modified electrodes; the concentration of luminol in the electrolyte was 5 mmol / L, the concentration of sulfuric acid was 0.5 mol / L, and the electrochemical deposition was performed by cyclic voltammetry for 25 cy...

Embodiment 3

[0069] Example 3 A chlordimeform enzyme-free sensor based on a molybdenum sulfide composite material, the specific preparation steps are:

[0070] (1) Zn-MoS 2 Preparation of:

[0071] The preparation steps are the same as that of Zn-MoS in Example 1 2 The method of preparation of the solution differs in: Zn Cl 2 The addition amount of ascorbic acid solution is 0.01 g, the addition amount of ascorbic acid solution is 6 mL, Na 2 MoO 4 The amount of solution added was 1.5 mL and Na 2 The amount of S added was 0.03 g, and the reaction was carried out at 220° C. for 12 hours.

[0072] (2) Preparation of ECL working electrode W1:

[0073] The preparation steps are the same as those of W1 in Example 1, except that the Zn-MoS prepared in (1) in this example is used 2 Modified electrode; the concentration of luminol in the electrolyte is 10 mmol / L, the concentration of sulfuric acid is 1.0 mol / L, and the electrochemical deposition is performed by cyclic voltammetry, and the cyc...

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Abstract

The invention discloses a preparation method of a biological immunosensor with electrochemiluminescence and photoelectrochemical dual signal development strategies constructed based on functional nanomaterials. Rapid and sensitive detection of ciprofloxacin in daily production, life and other fields.

Description

technical field [0001] The invention relates to a preparation method of a sensor for detecting dimeform, and the sensor integrates dual functions of electrochemiluminescence and photoelectrochemistry. It belongs to the field of new nano functional materials and electrochemical biosensing analysis technology. Background technique [0002] Dimeform is a kind of pesticide. In the soil, dimeform can migrate slightly to the deep layer of the soil through the leaching of water. Animals with chronic poisoning of dimeform may experience weight loss, hematocrit, hemoglobin and red blood cell count decrease, white blood cell count increase, etc. According to a 1972 WHO report, long-term exposure to chlordimeform can lead to cancer. [0003] At present, the methods for detecting dimeform mainly include chromatography and mass spectrometry. Such methods require expensive instruments and complex operations, and laboratory personnel need professional training before they can perform det...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/48G01N21/76B82Y30/00B82Y40/00
CPCB82Y30/00B82Y40/00G01N21/76G01N27/48
Inventor 张勇邓保军孙旭王耀光杜斌
Owner UNIV OF JINAN
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