Preparation method and application of a dual-functional ethinyl estradiol sensor based on electrochemiluminescence and photoelectrochemistry

A photoelectrochemical and electrochemical technology, applied in the fields of chemiluminescence/bioluminescence, electrochemical variables of materials, and analysis by chemical reactions of materials, etc. Achieve high selectivity detection, easy operation, improve detection sensitivity and detection limit effect

Inactive Publication Date: 2018-08-03
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, since electrochemiluminescence sensors require external light signal capture devices such as photodiodes, while photoelectrochemical sensors require external light sources to excite photosensitive materials, this affects the convenience of both operations to a certain extent and limits They are more widely used in actual production and life

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 An ethinyl estradiol sensor based on electrochemiluminescence and photoelectrochemical dual functions, the specific preparation steps are:

[0037] (1) Preparation of electrochemiluminescence working electrode W1:

[0038] 1) Using ITO conductive glass as the working electrode, Au@NiPd NRs was drop-coated on the surface of the electrode, covering an area of ​​1 cm × 1 cm, and dried at room temperature;

[0039] 2) Immerse the working electrode obtained in 1) into the electrolyte, and the immersion area is the area covered by Au@NiPd NRs. The working electrode is electrochemically deposited using a three-electrode system. After deposition, the working electrode is taken out and cleaned with ultrapure water. , and dried at 4°C in the dark to prepare the electrochemiluminescence working electrode W1;

[0040] The Au@NiPd NRs is an aqueous solution of rod-shaped nanomaterials with a core-shell structure, and the rod-shaped nanomaterials with a core-shell structure ...

Embodiment 2

[0055] Example 2 An ethinyl estradiol sensor based on electrochemiluminescence and photoelectrochemical dual functions, the specific preparation steps are:

[0056] (1) Preparation of electrochemiluminescent working electrode W1:

[0057] The preparation steps are the same as those of W1 in Example 1, except that the length of the gold nanorods is 30 nm, the concentration of luminol in the electrolyte is 5 mmol / L, the concentration of sulfuric acid is 0.5 mol / L, and the cyclic voltammetry When the electrochemical deposition method is used, the cycle is 25 times.

[0058] (2) Preparation of photoelectrochemical working electrode W2:

[0059] 1) Use ITO conductive glass as the working electrode, drop-coat 10 µL TiO on the surface of the electrode 2 NSs, dry at room temperature;

[0060] 2) Put the working electrode obtained in 1) into a muffle furnace, perform annealing treatment at 450 °C, and cool to room temperature after treatment;

[0061] 3) Apply 10 µL of the ethinyl...

Embodiment 3

[0065] Example 3 An ethinyl estradiol sensor based on electrochemiluminescence and photoelectrochemical dual functions, the specific preparation steps are:

[0066] (1) Preparation of electrochemiluminescent working electrode W1:

[0067] The preparation steps are the same as those of W1 in Example 1, except that the length of the gold nanorods is 40 nm, the concentration of luminol in the electrolyte is 10 mmol / L, the concentration of sulfuric acid is 1.0 mol / L, and the cyclic voltammetry When the electrochemical deposition method is used, the cycle is 30 laps.

[0068] (2) Preparation of photoelectrochemical working electrode W2:

[0069] 1) Using ITO conductive glass as the working electrode, drop-coat 12 µL TiO on the surface of the electrode 2 NSs, dry at room temperature;

[0070] 2) Put the working electrode obtained in 1) into a muffle furnace, perform annealing treatment at 450 °C, and cool to room temperature after treatment;

[0071] 3) Apply 12 µL of the ethin...

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Abstract

The invention discloses a preparation method of a bioimmune sensor with dual signal development strategies of electrochemiluminescence and photoelectrochemistry constructed on the basis of functional nanomaterials. The prepared sensor is simple to operate, easy to carry, fast to detect, and low in cost, and can be used in Rapid and sensitive detection of ethinyl estradiol in daily production, life and other fields.

Description

technical field [0001] The invention relates to a preparation method of a sensor for detecting ethinyl estradiol, and the sensor integrates dual functions of electrochemiluminescence and photoelectrochemistry. It belongs to the field of new nano functional materials and electrochemical biosensing analysis technology. Background technique [0002] Environmental estrogen refers to a class of compounds that interfere with the synthesis, release, transport, binding, and metabolism of normal endocrine substances in the body after entering the body, activate or inhibit the function of the endocrine system, and thereby destroy the stability and regulation of the body. There are many kinds of environmental estrogens, including synthetic compounds and plant natural estrogens, which are widely distributed in nature. Estrogen estradiol is a synthetic estrogen that is a steroid. At present, the methods for detecting ethinyl estradiol mainly include chromatography and mass spectrometry...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76G01N27/30
CPCG01N21/76G01N27/305
Inventor 颜兆庆张勇马洪敏魏琴杜斌
Owner UNIV OF JINAN
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