Kit for detecting nuclear factor-kappa B and application of kit

A nuclear factor and kit technology, applied in the field of nuclear factor-κB detection kits, can solve the problems of low sensitivity, complicated design, and long time consumption, and achieve high sensitivity, low detection limit, and overcome interference
CN112114154AActive Publication Date: 2020-12-22JIANGSU INST OF NUCLEAR MEDICINE
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGSU INST OF NUCLEAR MEDICINE
Publication Date
2020-12-22

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Abstract

The invention provides a probe capable of detecting a nuclear factor-kappa B with high sensitivity, good specificity and short consumed time. The probe is a DNA double-stranded probe; the DNA double-stranded probe is formed by reverse complementation of DNA1 and DNA2; and the nucleotide sequence of the DNA1 is as shown in SEQ ID No. 1, and the nucleotide sequence of the DNA2 is as shown in SEQ IDNo. 2. The invention develops a simple, convenient and sensitive nuclear factor kappa B detection method. According to the method, DNA binding protein, exonuclease III (ExoIII) and an isothermal indexamplification technology are combined, and dual amplification of signals is successfully realized by adopting a molecular beacon dependent amplification fluorescence analysis technology. Compared with other methods, the method has higher specificity and lower detection limit, and can be directly used for detecting the nuclear factor kappa B in a cancer cell nuclear extracting solution.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a kit for detecting nuclear factor-κB and its application. Background technique

[0002] At present, severe acute respiratory syndrome coronavirus (SARS-CoV-2) has swept the world, causing serious harm and impact on human health. The autopsy results showed that the pathological features of patients with new coronary pneumonia (COVID-19) suggested the occurrence of acute respiratory distress syndrome (ARDS), and the cause of ARDS was cytokine storm. When the virus invades cells, the release of nucleic acid RNA activates Toll-like receptor 7 (TLR7) and recruits multiple proteins to form a complex, thereby promoting transcription factors (TFs) such as interferon regulatory factor 7 (IRF7) and nuclear factor-κB ( NF-κB) enters the nucleus and then activates the expression of pro-inflammatory cytokines, causing the immune system to become overactive, causing a cytokine storm, which is of...

Claims

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