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Production method and application of ractopamine electrochemistry biology immunosensor

A technology of ractopamine and biological immunity, which is applied in the direction of chemiluminescence/bioluminescence, electrochemical variables of materials, and analysis by making materials undergo chemical reactions, can solve the problems affecting the convenience of operation and restricting wide application, etc., to achieve Easy operation, high selectivity detection, improved detection sensitivity and detection limit effect

Inactive Publication Date: 2016-07-20
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, since the electrochemiluminescence sensor requires external light signal capture devices such as photodiodes, and the photoelectrochemical sensor requires an external light source to excite the photosensitive material, this affects the convenience of the operation of the two to a certain extent and limits them. Wider application in actual production and life

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 A ractopamine electrochemical bioimmunosensor, the specific preparation steps are:

[0042] (1) Preparation of electrochemiluminescence working electrode W1:

[0043] 1) Using ITO conductive glass as the working electrode, drip NH on the electrode surface 4 NiPO 4 Solution, covering an area of ​​1cm×1cm, dry at room temperature;

[0044] 2) Put the working electrode obtained in 1) in NH 4 NiPO 4 AuAg coating on the surface 2 SNRs, covering an area of ​​1cm×1cm, dry at room temperature;

[0045] 3) Immerse the working electrode obtained in 2) into the electrolyte, the immersion area is NH 4 NiPO 4 And AuAg 2 In the area covered by SNRs, the working electrode is electrochemically deposited using a three-electrode system. After deposition, the working electrode is taken out, cleaned with ultrapure water, and dried at 4°C in the dark to prepare the electrochemiluminescence working electrode W1;

[0046] The NH 4 NiPO 4 The solution is a nickel ammonium phosphate micro-nano ...

Embodiment 2

[0064] Example 2 A ractopamine electrochemical bioimmunosensor, the specific preparation steps are:

[0065] (1) Preparation of electrochemiluminescence working electrode W1:

[0066] The preparation steps are the same as the preparation steps of W1 in Example 1, except that in the preparation steps of the nickel ammonium phosphate micro-nano material, the amount of ammonium salt added is 0.3g, the amount of phosphate added is 0.20g, and the amount of nickel dichloride added is 0.20g, stirring temperature and time are 38℃ and 12h respectively; the length of gold nanorods is 30nm; the concentration of luminol in the electrolyte is 5mmol / L, and the concentration of sulfuric acid is 0.5mol / L; cyclic voltammetry for electrochemistry When depositing, circulate 25 times.

[0067] (2) Preparation of photoelectrochemical working electrode W2:

[0068] 1) Using ITO conductive glass as the working electrode, drip 10μL TiO on the electrode surface 2 NSs, dry at room temperature;

[0069] 2) Put ...

Embodiment 3

[0074] Example 3 A ractopamine electrochemical bioimmunosensor, the specific preparation steps are:

[0075] (1) Preparation of electrochemiluminescence working electrode W1:

[0076] The preparation steps are the same as the preparation steps of W1 in Example 1, except that in the preparation step of the nickel ammonium phosphate micro-nano material, the amount of ammonium salt added is 0.4g, the amount of phosphate added is 0.25g, and the amount of nickel dichloride added is 0.25g, stirring temperature and time are 45℃ and 14h respectively; the length of gold nanorods is 40nm; the concentration of luminol in the electrolyte is 10mmol / L, the concentration of sulfuric acid is 1.0mol / L; cyclic voltammetry is used for electrochemistry When depositing, circulate 30 times.

[0077] (2) Preparation of photoelectrochemical working electrode W2:

[0078] 1) Using ITO conductive glass as the working electrode, drip 12μL TiO on the electrode surface 2 NSs, dry at room temperature;

[0079] 2) ...

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Abstract

The invention discloses a production method of a biology immunosensor having double signal amplification strategies of electrochemiluminescence and photoelectrochemistry, which is built based on a functional nanometer material. The produced immunosensor is simple to operate, convenient to carry, quick in detection, and low in cost, and can be used for quick and acute detection in the fields of daily production, living and the like.

Description

Technical field [0001] The invention relates to a preparation method of a telephone biological immunosensor for detecting ractopamine. The sensor integrates the dual functions of electrochemiluminescence and photoelectrochemistry. It belongs to the technical field of new nano-functional materials and electrochemical biosensing analysis. Background technique [0002] Ractopamine is a synthetic clembaan beta adrenergic receptor agonist, used by some pig farms as a new type of clenbuterol. Because eating large amounts of meat or internal organs containing ractopamine residues may cause people to have symptoms such as breathing difficulties, nausea, dizziness, palpitations, and increased blood pressure. In severe cases, it may affect the reproductive system. Therefore, the use of ractopamine in China has been banned from February 5, 2013. [0003] At present, the main methods for detecting ractopamine include chromatography and mass spectrometry. This kind of method is expensive and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N21/76G01N27/30G01N27/327
CPCG01N21/76G01N27/305G01N27/327G01N33/54306G01N33/54386
Inventor 张勇魏琴杜斌马洪敏范大伟
Owner UNIV OF JINAN
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