Detection method of Staphylococcus aureus in milk with double signal amplification

A technology of amplification and dual signals, applied in the field of biosensing, can solve the problems of high technical requirements for operators, complicated PCR technology operation, and high cost, and achieve the effects of high sensitivity, simple operation, and low cost

Active Publication Date: 2020-08-28
JIANGSU UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

PCR technology is complicated to operate, high in cost, and requires high technical requirements for operators, etc.

Method used

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  • Detection method of Staphylococcus aureus in milk with double signal amplification
  • Detection method of Staphylococcus aureus in milk with double signal amplification

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Embodiment Construction

[0034] Below in conjunction with accompanying drawing and embodiment the present invention is described in further detail, and concrete operation method is as follows:

[0035] (1) Preparation of gold nanoparticles (AuNPs)

[0036] Take 50mL 0.3mM HAuCl 4 ·6H 2 Heat the O solution to a boil with constant stirring, add 0.75mL of 1% sodium citrate, continue to boil for 4min until the color turns wine red and remains unchanged, cool to room temperature, and store in the refrigerator.

[0037] (2) Preparation of graphene-gold nanoparticles nanocomposites (GO-AuNPs)

[0038] Mix 1mL of 0.5mg / mL carboxylated graphene oxide (GO) solution with 2mL of 1% polydiallyldimethylammonium chloride (PDDA) solution and stir for 0.5h to make it evenly mixed, then centrifuge and wash, Then 2 mL of the above AuNPs solution was added and stirred for 0.5 h to make it evenly mixed, then centrifuged and washed and stored in a refrigerator at 4°C.

[0039] (3) Preparation of gold-platinum nanocompo...

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Abstract

The invention discloses a detection method of staphylococcus aureus in milk through a double signal amplification effect and belongs to the technical field of biosensing. According to the technology,a gold-platinum nano composite material and DNA mimic enzyme are used for synthesizing nano probes which have the good double catalytic effect on H2O2, a modified electrode made of a graphene / gold nanoparticle nano composite material is used so that the electron transfer capacity of the modified electrode can be improved, and accordingly quick high-sensitivity detection for staphylococcus aureus is achieved. Under the optimized experimental conditions, an electrochemical immunosensor has a wide linear detection range for staphylococcus aureus. In addition, the sensor is successfully applied toquantitative detection of staphylococcus aureus in pure milk and yogurt samples. Compared with traditional methods such as a slab colony counting method, the detection method is convenient and fast,easy to operate and high in sensitivity, and quick high-sensitivity detection for staphylococcus aureus is achieved.

Description

technical field [0001] The invention provides an electrochemical immunosensor based on double signal amplification of nanomaterials and DNA imitating enzymes to realize highly sensitive detection of Staphylococcus aureus in milk, belonging to the technical field of biosensing. Background technique [0002] Food safety is a public health issue that has attracted global attention. With the gradual expansion of food production scale, the obvious manifestation is the continuous outbreak of food safety incidents in the world in the past period of time, which caused various food-borne diseases. Disease is one of them. Foodborne diseases not only cause serious harm to human health, but also cause a lot of social and economic losses. Staphylococcus aureus can be found everywhere in nature, and can be found in water, air and excrement. It is one of the most important food-borne pathogens. It can cause superficial skin infection, soft tissue infection, lung infection, sepsis and oth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/36G01N27/30G01N33/569
CPCG01N27/308G01N27/3277G01N27/3278G01N27/36G01N33/56938G01N2333/31
Inventor 韩恩程喜红丁文龙蔡健荣
Owner JIANGSU UNIV
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