Test paper for identifying and detecting virulent strain and low virulent strain of hog cholera virus

A swine fever virus, differential detection technology, applied in the field of swine fever virus virulent and weak virulent differential detection test strips, can solve problems such as difficulty in distinguishing immune weak and wild virus infection, lack of differential diagnosis methods, and unfavorable CSF purification, and achieves easy large-scale Scope of promotion and application, easy and fast operation, high mark rate effect

Active Publication Date: 2015-03-11
HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the prevention and control of swine fever in my country still adopts a strategy based on vaccine immunization. HCLV vaccine is a world-recognized excellent vaccine developed in the 1950s in my country. It has good safety, good immunogenicity, and stable genetic characteristics. It can be used simultaneously Inducing humoral immunity and cellular immunity can produce immune protection against different gene subgroups (1.1-3.4 subgroups). However, due to the lack of serological markers and supporting differential diagnosis methods, its large-scale application in my country makes it difficult to pass antibody detection. Difficult to distinguish between immune attenuated virus and wild virus infection, which is not conducive to the purification of CSF
With the completion of the complete gene sequences of CSFV wild strains and vaccine strains from different sources and

Method used

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  • Test paper for identifying and detecting virulent strain and low virulent strain of hog cholera virus
  • Test paper for identifying and detecting virulent strain and low virulent strain of hog cholera virus

Examples

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Embodiment 1

[0064] Example 1, rapid diagnosis of virulent classical swine fever infection, collect diseased tissues of sick (dead) pigs, including lung, liver, spleen, kidney, trachea, small intestine, large intestine and lymph nodes, etc., add appropriate amount at 1:5 or 1:10 Simply grind PBS or water to prepare the solution to be tested, use test paper to identify strong and weak strains of CSFV according to the operation method (15) for detection and result judgment, and differentially diagnose sick (dead) pigs with strong virus infection or vaccine immunity . The test paper shows three reddish-brown bands (strong virus detection line, weak virus test line and quality control line) "|||" is positive for strong CSFV virus, indicating that the pigs to be tested are infected with strong CSFV virus; two reddish-brown bands (weak virus Detection line and quality control line) "||" is CSFV attenuated positive, indicating that the pigs to be tested are immune to CSFV vaccine; only one reddis...

Embodiment 2

[0065]Example 2, swine fever virus inspection and quarantine of pigs, collect pig swabs (throat swab and anal swab) or feces, add appropriate amount of PBS or water at 1:5 or 1:10 for simple suspension, prepare the solution to be tested, and use The detection test paper for the identification of strong and weak strains of CSFV is carried out according to the operation method (15) and the results are judged to identify and detect the strong virus pollution or vaccine immunity of pigs. Three reddish-brown bands appear on the test paper (strong toxin detection line, weak toxin detection line and quality control line) "|||" is positive for strong CSFV virus, indicating that the pigs to be tested or the environment are highly toxic to CSFV pollution; two reddish-brown bands (Attenuated virus detection line and quality control line) "||" is positive for CSFV attenuated virus, indicating that the pigs to be inspected or the environment are free of CSFV virulence pollution, and they ar...

Embodiment 3

[0066] Embodiment 3, the quality detection of the attenuated swine fever vaccine, add appropriate amount of PBS or water to dissolve the attenuated live vaccine of swine fever at 1:5 or 1:10, including milk rabbit vaccine, cell vaccine and lymphocytic vaccine, etc., to prepare the vaccine solution to be tested , use test paper to identify strong and weak strains of CSFV according to the operation method (15) for detection and result judgment, to detect the virus content of the attenuated CSF vaccine and to identify and detect the strong virus contamination of the vaccine. The test paper shows three reddish-brown bands (strong virus detection line, weak virus test line and quality control line) "|||" is positive for CSFV strong virus, indicating that the vaccine to be tested is contaminated by CSFV strong virus; two reddish-brown bands (weak virus Detection line and quality control line) "||" is positive for attenuated CSFV, indicating that the vaccine to be tested has no strong...

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Abstract

The invention relates to livestock epidemic disease infection and immune identification and detection instruments, and in particular relates to a piece of test paper for identifying and detecting a virulent strain and a low virulent strain of hog cholera viruses. The test paper consists of a support plate, a sample pad, a gold mark pad, a detection membrane and a water absorbing pad, wherein a virulent infection detection line T1 '|', a low virulent infection detection line T2 '|' and a quality control line C '|' are arranged on the detection membrane. When the test paper is used, three red strips '|||' mean virulent virus infection of hog cholera viruses, two red strips '||' mean vaccine immunity of hog cholera viruses, and one red strip '|' means hog cholera viruse negative. The test paper is high in specificity, high in sensitivity, wide in reaction spectrum and simple, convenient and rapid to operate, can be used for detecting conventional low virulent vaccine strains and multiple epidemic virulent strains, can be widely used for identifying and detecting hog cholera virus infection and immune, and can be easily popularized and applied in production practice.

Description

technical field [0001] The invention relates to a detection device for identification and immunity of livestock epidemic diseases, in particular to a test paper for identification and detection of strong and weak swine fever virus. Background technique [0002] Classical swine fever (CSF) is a highly contagious disease characterized by high fever, hemorrhage and high mortality caused by classical swine fever virus (CSFV), the World Organization for Animal Health (OIE) It is included in the OIE Epidemic Disease List as an animal infectious disease that must be declared, and it is listed as a first-class animal epidemic disease in my country. my country has used hog cholera lapinized vaccine (HCLV) vaccine to prevent and control the large-scale epidemic of CSF. Sexual infection, so-called "atypical swine fever", "mild swine fever" and "venomous sow syndrome" have appeared, which are still one of the main infectious diseases that endanger the pig industry. CSFV belongs to the F...

Claims

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Application Information

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IPC IPC(8): G01N33/569
CPCG01N33/558G01N33/56983G01N33/577G01N2333/183
Inventor 郭军庆邢广旭王丽孙亚宁杨继飞杨艳艳郅玉宝柴书军李青梅王瑞宁邓瑞广张改平
Owner HENAN ACAD OF AGRI SCI
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