45 results about "Base analog" patented technology

Compositions and methods for forming hydrocarbon products from triglycerides are disclosed. In one aspect, the methods involve the thermal decomposition of fatty acids, which can be derived from the hydrolysis of triglycerides. The thermal decomposition products can be combined with low molecular weight olefins, such as Fischer-Tropsch synthesis products, and subjected to molecular averaging reactions. Alternatively, the products can be subjected to hydrocracking reactions, isomerization reactions, and the like. The products can be isolated in the gasoline, jet and / or diesel fuel ranges. Thus, vegetable oils and / or animal fats can be converted using water, catalysts, and heat, into conventional products in the gasoline, jet and / or diesel fuel ranges. These products are virtually indistinguishable from those derived from their petroleum-based analogs, except that they can have virtually no aromatic, sulfur or nitrogen content, they are derived, in whole or in part, from renewable resources, and can also be derived from domestically available coal and / or natural gas.

The invention relates to the generation and characterization of Archaeal DNA polymerase mutants with deficient 3′-5′ exonuclease activity and reduced base analog detection activity. The invention further provides for Archaeal DNA polymerase mutants with deficient 3′-5′ exonuclease activity and reduced base analog detection activity containing additional mutations that modulate other DNA polymerase activities including DNA polymerization or reverse transcriptase activity. The invention also discloses methods and applications of DNA polymerases with deficient 3′-5′ exonuclease activity and reduced base analog detection activity.

A system and method of designing the physical layout of an SoC incorporating row-based placement of analog standard cells whose heights are constrained to a predetermined row height or integer multiple thereof. A library of analog standard cells may be utilized by an ECAD tool to map, place, and route analog and mixed signal circuits in a manner similar to how such ECAD tool may utilize a library of digital standard cells to map, place, and route digital circuits. Mapping, placing, and routing of digital, analog, and mixed signal circuits may proceed within a unified ECAD SoC physical design flow. Finally, a general type analog standard cell is taught to further increase the speed and efficiency of analog and mixed-signal SoC layout.

The invention relates to a complete digital electric energy meter quantity value tracing system based on analog quantity detection and a digital electric energy meter quantity value tracing method based the analog quantity detection. The digital electric energy meter quantity value tracing system based on the analog quantity detection includes an analog electric energy device, an analog standard electric energy meter, an analog standard power meter, a voltage and current conversion message module, a standard power calculation and standard electric energy generation module, and three error calculation modules; the voltage and current conversion message module samples voltage and current parameters of the analog electric energy device, and transmits an IEC61850 message under sequential logic control; and the standard power calculation and standard electric energy generation module samples the content of the IEC61850 message, computes the power of the IEC61850 message real time, and generates electric energy, and performs display and pulse output. With the digital electric energy meter quantity value tracing system based on the analog quantity detection of the invention adopted, quantity values of digital electric energy meters can be traced back to analog quantity measurement standards through an improved method, and the technical problem of difficult tracing of the digital quantity of the digital electric energy meters can be solved, and an improved solution is provided for the tracing of the quantity values of the digital electric energy meters.

Various systems and methods for capturing data are disclosed. For example, some embodiments of the present invention provide methods for performing a first analog to digital conversion using a delta-sigma based analog to digital converter, and performing a second analog to digital conversion using a SAR based analog to digital converter. The delta-sigma converter provides a first portion of a conversion result, and the SAR based analog to digital converter provides a second portion of the conversion result. The methods further include combining the first portion of the conversion result with the second portion of the conversion result to produce a combined conversion result.

The invention discloses a reagent, detection method and application for the detection of African hog cholera virus. The reagent comprises a specific primer pair and a probe, upstream and downstream primers of the primer pair are respectively sequences shown in Seq ID No. 1 and 2, and the probe is a sequence shown in Seq ID No. 3 or its reverse complement sequence; in the probe sequence shown in the SEQ ID No.3, a 28th base modifies fluorescent quenching group-dT, a 31st base is replaced with a base analog, a 33rd base modifies a fluorescent group-dT, and a 3' end modifies C3 Spacer. The reagent is sensitive, specific and efficient for detection of the African hog cholera virus by recombinase polymerase amplification; compared with conventional conventional or real-time fluorescent PCR, thereagent and the detection method have short detection time and simple operation, are especially suitable for on-site testing, and are of great significance for the rapid prevention and control of theAfrican hog cholera virus and guarantee of production safety.

The invention discloses a genetic programming-based analog circuit fault test optimal sequential search method. A plurality of fault diagnosis trees are firstly randomly generated according to a test dependency matrix of a modular circuit, a genetic programming method is adopted to carry out selection, crossover and mutation on the fault diagnosis trees, a reciprocal of the test cost corresponding to the fault diagnosis tree serves as the individual fitness of the fault diagnosis tree, a fault rally point and a sub tree are exchanged only during the crossover process according to features of the fault diagnosis tree, a testing point is only selected for mutation during the mutation process, a sub tree under the testing point and the rally point is generated again, the fault diagnosis tree with the minimal test cost is selected from the current plurality of fault diagnosis trees after multiple times of iteration to serve as the final fault diagnosis tree. Through accurate search, a testing point sequence isolating the maximal fault points at the minimal test cost can be obtained, and guidance is provided for modular circuit fault test.

The invention provides an imager readout architecture utilizing analog-to-digital converters (ADC), the architecture comprising a band-limited sigma delta modulator (SDM) ADC; and a serpentine readout, which can be configured to allow the band-limited SDM to multiplex between multiple columns by avoiding discontinuities at the edges of a row. SDM ADC image reconstruction artifacts are minimized using a modified serpentine read out methodology, the methodology comprising using primary and redundant slices with the serpentine read out in opposite directions and averaging the slices. Advantageously, the invention can be used to develop a read out integrated circuit (ROIC) for strained layer superlattice imagers (SLS) using sigma delta modulator (SDM) based analog to digital converters (SDM ADC).

The present invention relates to an oligonucleotide for analyzing a target nucleotide sequence by hybridization and its applications. The oligonucleotide has the following general structure: 5′-Xp—Yq-Zr-3′ or 5′-Zr—Yq-Xp-3′ wherein Xp represents a first hybridization portion having a specific hybridizing nucleotide sequence substantially complementary to said target nucleotide sequence in said sample nucleic acid to hybridize therewith; Yq represents a regulator portion comprising at least two universal bases or non-discriminatory base analogs; Zr represents a second hybridization portion having a pre-selected arbitrary nucleotide sequence; p, q and r represent the number of nucleotides; and X, Y and Z is deoxyribonucleotide or ribonucleotide.

The invention relates to a neural-network-based analog simulation system of vehicle gas emissions. The system includes a basic simulation model building module, a neural network input data processingmodule, a neural network module, a neural network training module and an emission calculation and output module. Firstly, the basic simulation model building module is used for building modules for engines, transmission systems and the overall vehicle; secondly, the neural network input data processing module is used for simulation of the modules, and original emission data is obtained. The original emission data, catalytic converter parameters and the emission influence conditions are combined, and input data of the neural network training module is obtained. The neural network training module completes analog calculation on the input data, and final emission data of the overall vehicle is obtained; finally, special working conditions are added in the emission calculation and output module for correction of the final emission data. Through the combination of the neural network technology and the generation and transformation rule of the emissions of the overall vehicle during the running test cycle, the emission result of the whole vehicle is accurately predicted.

The present invention relates to an annealing control primer for improving annealing specificity in nucleic acid amplification and its applications to all fields of nucleic acid amplification-involved technology. The present primer comprises (a) a 3'-end portion having a hybridizing nucleotide sequence substantially complementary to a site on a template nucleic acid to hybridize therewith; (b) a 5'-end portion having a pre-selected arbitrary nucleotide sequence; and (c) a regulator portion positioned between said 3'-end portion and said 5'-end portion comprising at least one universal base ornon-discriminatory base analog, whereby said regulator portion is capable of regulating an annealing portion of said primer in association with annealing temperature.

A process for determining the sequence of nucleic acids of interest employs nucleotides or nucleotide analogs that have been made detectable by non-radioactive modifying or labeling. Such nucleotides or nucleotide analogs are modified on the sugar moieties, the phosphate moieties or the base moieties, including base analogs. Modified nucleotide analogs can be attached to or coupled to or incorporated into DNA or RNA. The modified or labeled nucleotides or nucleotide analogs are also useful in processes for detecting the presence of nucleic acids of interest and for characterizing chromosomal sequences. Detection processes using the modified or labeled nucleotides or nucleotide analogs extend to the use of a gel for separating or resolving hybrids formed between non-radioactively labeled oligonucleotides or polynucleotides and such nucleic acids of interest.

Various thienopyrimidine-based analog compounds are able to selectively inhibit the Src family of tyrosine kinases. Compounds of the present invention, capable of such selective inhibition, are of the basic structure seen in formulae (I), (II) or (III):These compounds are useful in the treatment of a wide variety of diseases including hyperproliferative diseases, hematologic allergic / immunological diseases, or viral infections. Methods of synthesis of these compounds and their methods of inhibiting the Src family of tyrosine kinases are presented.

The invention discloses an FPGA-based portable electrical impedance imaging system and a working method thereof. Imaging is mainly performed according to the characteristic of different conductivity of different biological tissue or the same biological tissue in different physiological states, and distribution information of conductivity in the tissue is reflected. A Red pitaya development board kit based on the ZYNQ series FPGA chip is adopted as the core, Red pitaya OS runs in embedded main control, and a board is loaded with a wireless / wired network module, a DDS signal generator, a signal conditioning module, an operational amplifier and filter circuit and a data acquisition circuit, and a voltage-controlled constant current source, a C51-based analog multiplexer with variable channel number and an electrode sensor are matched to be adopted as peripheral measurement equipment. High-precision, informationized and intelligent biological tissue information detection is realized by PC-side STEM lab development environment and Matlab software. A solution with high imaging quality, high accuracy rate and portability is provided by fully considering problems such as EMC.

The embodiment of the invention discloses an FPGA (field programmable gate array)-based analog video ADC (analog to digital converter) automatic adjustment method and device. The method comprises the following steps: presetting the ideal field value range; obtaining actual field values of light colors according to the preset obtaining rule; and when the actual field values of the light colors are not in the ideal field value range, adjusting the offset coefficients and gain coefficients corresponding to the light colors according to the preset adjustment rule until the adjusted actual field values of the light colors are in the ideal field value range, wherein the light colors are at least one of red R, green G or blue B. The method and the device disclosed by the embodiment of the invention have the following beneficial effects: digital automatic adjustment can be implemented; the effect precision after adjustment is high; the adjustment results are unified and reliable; and the adjustment time is greatly shortened.

Disclosed herein are novel platinum-based analogs with a single substituted imine ligand: R7RC═NR8, wherein the R7RC═NR8 functional group is covalently bonded to the platinum through nitrogen. The analogs also have nitrogen donor ligands capable of forming hydrogen bonds with the bases in DNA or RNA, and one or more leaving groups which can be displaced by water, hydroxide ions or other nucleophiles, which is thought to form active species in vivo, and then, form cross-linked complexes between nucleic acid strands, principally between purines in DNA (or RNA), i.e., at the Guanine or Adenine bases, thereof. These platinum analogs may also be more easily transported into tumor cells, due to their increased lipophilicity and are likely to be useful as anti-neoplastic agents, and in modulating or interfering with the synthesis or replication or transcription of DNA or translation or function of RNA in vitro or in vivo, as they are potentially capable of forming a platinum coordinate complex with a intact or nascent DNA or RNA and thereby interfering with cellular synthesis, transcription or replication of nucleic acid polynucleotides.

The present invention provides improved methods for generating fluorescent aptamer polynucleotides, novel polynucleotides, and methods for use thereof.

The invention discloses a method for monitoring number of cell division, and an application thereof. The method for assistant monitoring the number of the cell division comprises the following steps: (1) adopting base analogs for marking a cell to be monitored, wherein a single-stranded DNA of all double-stranded DNA of the marked cell to be monitored is marked with the base analog after marking,the marked cell to be monitored is recorded as a undivided initial cell, a base analog marked chromosome is recorded as a marked chromosome; (2) determining division number of the initial cell according to the following method 1) or the method 2): 1) observing the marked chromosome in the daughter cell generated from the division of the initial cell, then observing and counting the amount of the marked chromosome in single daughter cell if each cell of the daughter cell contains the marked chromosome, and obtaining the division number of the initial cell according to the amount of the marked chromosome in the single daughter cell. The method provides correct and reliable result, and does not require extra operations during tracking except routine cell culture, routine animal feeding and final cell dyeing operation. Therefore, the method provided by the present invention has advantages of simple operation, and time saving and labour saving.

The present invention relates to an annealing control primer for improving annealing specificity in nucleic acid amplification and its applications to all fields of nucleic acid amplification-involved technology. The present primer comprises (a) a 3′-end portion having a hybridizing nucleotide sequence substantially complementary to a site on a template nucleic acid to hybridize therewith; (b) a 5′-end portion having a pre-selected arbitrary nucleotide sequence; and (c) a regulator portion positioned between said 3′-end portion and said 5′-end portion comprising at least one universal base or non-discriminatory base analog, whereby said regulator portion is capable of regulating an annealing portion of said primer in association with annealing temperature.

The application discloses a reagent, a detection method and an application for detecting deer epidemic hemorrhagic fever virus. The reagent comprises a specific primer pair and a probe, wherein upstream and downstream primers of the primer pair are shown in SEQ ID No. 1 and 2 respectively, and the probe is shown in SEQ ID No. 3; and in the sequence shown in SEQ ID No. 3, a 32nd base modifies fluorescence quenching group-dT, a 33rd base is replaced by base analog, a 35th base modifies fluorescence group-dT, and a 3' end modifies C3Spacer. The reagent can sensitively, specifically and efficiently detect deer epidemic hemorrhagic fever virus through one-step reverse transcription recombinase polymerase amplification. By adopting the reagent, on-site detection of deer epidemic hemorrhagic fever virus can be conveniently carried out, so that the detection result can be quickly obtained; and the regent is of great significance for the rapid prevention and control of deer epidemic hemorrhagic fever virus, preventing the spread of the epidemic, and ensuring production safety to the greatest extent.

Disclosed herein are novel platinum-based analogs with a single substituted azole ligand: RN═NR7, wherein the RN═NR7 functional group is covalently bonded to the platinum through nitrogen of NR7. The analogs also have nitrogen donor ligands capable of forming hydrogen bonds with the bases in DNA or RNA, and one or more leaving groups which can be displaced by water, hydroxide ions or other nucleophiles, which is thought to form active species in vivo, and then, form cross-linked complexes between nucleic acid strands, principally between purines in DNA (or RNA), i.e., at the Guanine or Adenine bases, thereof. These platinum analogs may also be more easily transported into tumor cells, due to their increased lipophilicity and are likely to be useful as anti-neoplastic agents, and in modulating or interfering with the synthesis or replication or transcription of DNA or translation or function of RNA in vitro or in vivo, as they are potentially capable of forming a platinum coordinate complex with an intact or nascent DNA or RNA and thereby interfering with cellular synthesis, transcription or replication of nucleic acid polynucleotides.

The invention provides a bushing type electric fireplace reflective beam, which includes a reflective strip and a matrix for fixing the reflective strip. The matrix for fixing the reflective strip is composed of a mandrel and a rotating cylinder sleeved on the mandrel. The mandrel is fixed on the electric fireplace. Fireplace furnace body, the drum rotates along the center line of the mandrel and drives the reflective strips bonded or bundled on it to rotate, making the soft reflective strips form irregular jumps; the overall flame profile is simulated by various shapes of the base, and then superimposed reflective The irregular jumping of the strips makes the simulated flame shape of the reflected light on the imaging device richer, and the simulation effect is more realistic; the base of the fixed reflective strips also has the ability to reshape, that is, after use, the base can also be manually bent to form The flame profile that is more in line with the user's requirements makes the present invention expandable to simulated flames; meanwhile, the present invention also has the characteristics of low noise, simple structure, and low cost.

A method is provided for treating a patient having a disease associated with undesirable or uncontrolled cell proliferation, the method comprising: administering to the patient a 20(S)-camptothecin for a period of time during which a pyrimidine base analog is not being administered to the patient; and administering a pyrimidine base analog to the patient.

The invention discloses tylophora ovata base analogs with anti-tumor activity and a preparation method and a use thereof. The tylophora ovata base analogs can be applied in the preparation of drugs for treating malignant tumors, leukemia and cancers, and the compound structural formula is as follows: R1, R2, R3 or R4 can be CH3O, R1R2, R3R4 and OCH2O, and R1, R2, R3 and R4 can not be CH3O at the same time. The tylophora ovata base analogs have the characteristics of high efficiency, low toxicity and significant role of inhibiting tumor cells, and can be applied in anti-cancer new drugs; the tylophora ovata base analogs are synthesized by adopting the one kettle way, the process is not separated, and final products are separated; and PIDA is added for synthesis, the synthesis route is simple, the efficiency is high and the cost is low.