9 results about "Magnesium pyrophosphate" patented technology

The invention relates to a sliding material based on PTFE polymer with a filler to improve the friction properties, wherein the filler comprises at least one phosphate, in particular calcium phosphate, calcium pyrophosphate, magnesium phosphate, magnesium pyrophosphate, lithium phosphate, hydroxyapatite or combinations thereof, and at least one metal sulfide, wherein the proportion of metal sulfide is > 2%. The invention also relates to the use of the sliding material.

The present invention provides a universal fluorescent probing system and method for result readout of loop-mediated isothermal amplification (LAMP) or reverse transcription-loop-mediated isothermal amplification (RT-LAMP) in a closed-tube manner using at least three different types of universal quantum dot probes, including sulfonate-, carboxyl-, and amine-modified quantum dots (QDs). Detection mechanism of the present system and method relies on a co-precipitation of the modified QDs with magnesium pyrophosphate crystals (one of the positive LAMP / RT-LAMP reaction products) formed during the reaction. The present system and method are advantageous over the existing technologies in terms of simple preparation, low cost, as well as excellent universality, specificity and sensitivity.

The application discloses a dye-free naked-eye recognizable nucleic acid culture detection counting method, and belongs to the technical field of digital nucleic acid culture. The method comprises the following steps: (1) adding water gel monomers acrylamide and methylene bisacrylamide into a loop-mediated isothermal amplification reaction system of a to-be-detected sample, and initiating crosslinking to form a water gel system; (2) isothermal amplification reaction; (3) after the amplification is completed, a magnesium pyrophosphate precipitate point formed in the water gel system is recognized and counted, and the concentration of target nucleic acid molecules in the to-be-detected sample is calculated. The application combines polyacrylamide water gel and digital LAMP, utilizes the adsorption flocculation capacity of PAM to control the LAMP reaction by-product magnesium pyrophosphate precipitation in the vicinity of a nucleic acid amplification group, forms a white point which has a clear color difference with a background, and the position and size of the white point are basically consistent with those of the nucleic acid amplification group, and thus the dye-free naked-eye recognizable nucleic acid culture counting effect is realized.

The invention discloses a method for preparing a high-thermal-stability frit from glass waste, and relates to the technical field of ceramic frits, and the method comprises the following steps: reducing and sintering preparation raw materials to obtain the high-thermal-stability frit; the composite material is prepared from the following raw materials in parts by mass: 40-60 parts of waste glass powder, 10-20 parts of boron tail powder, 2-8 parts of a fixing agent and 1-5 parts of a phosphate mineralizer, the fixing agent is at least one of zirconium oxide, lanthanum oxide and tin oxide; and the phosphate mineralizer is aluminophosphate or magnesium pyrophosphate. The reduction sintering process comprises the following steps: firstly, raising the temperature to 750-850 DEG C in a reducing atmosphere, and keeping the temperature for 20-30 minutes; the temperature is increased to 1100-1250 DEG C, melting is conducted for 25-50 min, and finally the molten mass is quenched. The main raw materials are all glass wastes, the cost of the raw materials is low, and the prepared ceramic frit is high in thermal stability.

The invention provides a preparation method of magnesium pyrophosphate coated magnesium oxide. The preparation method comprises the following steps: firstly, sequentially carrying out ball-milling pretreatment on magnesium oxide, an ethanol-water mixed solution and a silane coupling agent, and forming a magnesium hydroxide layer and a silane hydrophobic protection layer on the surface of magnesium oxide to obtain pre-hydrolyzed magnesium oxide particles; and then mixing and ball-milling with a phosphorus-containing mineralizing agent, and carrying out high-temperature melting and calcining, so that the mineralizing agent reacts with the magnesium hydroxide layer to generate a compact magnesium pyrophosphate coating layer, namely the magnesium pyrophosphate-coated magnesium oxide is prepared. According to the preparation method disclosed by the invention, effective coating of magnesium pyrophosphate on magnesium oxide is successfully realized through a simple ball-milling and calcining process, the hydrolysis resistance is remarkably improved, and meanwhile, the obtained powder is good in dispersity and uniform in particle size, and has a wide application prospect in the field of high-thermal-conductivity composite materials.

The invention discloses an mRNA (messenger ribonucleic acid) preservation and purification method based on a coacervate, and belongs to the technical field of biology. The invention provides an mRNA (messenger Ribonucleic Acid) preservation method and purification method based on a condensation body. The preparation process of the mRNA aggregate is simple, the mRNA aggregate can be formed by directly mixing mRNA, magnesium pyrophosphate particles, magnesium ions and a glucan enrichment phase, and long-term storage of mRNA is achieved by freeze-drying the mRNA aggregate. The purified mRNA can be obtained by freeze-drying and preserving mRNA agglomerates formed by direct mixing, and then redissolving, centrifuging and filtering gel. Based on the protection effect of the aggregate on the mRNA, the method can realize long-term storage of the mRNA without carrying out LNP packaging on the mRNA; and the freeze-drying time of the condensation body is short, the post-treatment process is simple, and a research basis is provided for the development of a novel mRNA process route.

The present application relates to a kind of preparation method and application of high stability bioactive amorphous calcium magnesium phosphate.The preparation method includes the following steps: (1) preparation pyrophosphate aqueous solution;(2) soluble magnesium salt is dispersed into pyrophosphate aqueous solution, fully stirred, and pyrophosphate magnesium dispersion is prepared;(3) inorganic calcium salt is dispersed into pyrophosphate magnesium dispersion, and pH is adjusted to 10 or more, fully stirred, and colloidal substance is obtained, and high stability bioactive amorphous calcium magnesium phosphate is prepared by separating colloidal substance.The application of high stability bioactive amorphous calcium magnesium phosphate prepared is used for the preparation of bone defect support and the repair of bone defect.The beneficial effect is that pyrophosphate with complexing property realizes efficient loading and controllable release of calcium and magnesium ions.Pyrophosphate can be hydrolyzed into phosphate in vivo, and is an important substance involved in in vivo metabolism.The raw materials added in the whole preparation process are cheap and easy to obtain, simple and non-toxic, simple and green process, and simple operation.

The present invention discloses a method for preparing a high-thermal-stability frit using waste glass, which relates to the technical field of ceramic frits. The method comprises the following steps: reducing and sintering the raw materials to obtain the high-thermal-stability frit; the raw materials include, by weight, 40-60 parts of waste glass powder, 10-20 parts of boron tail powder, 2-8 parts of a fixative, and 1-5 parts of a phosphate mineralizer; the fixative is at least one of zirconium oxide, lanthanum oxide, and tin oxide; and the phosphate mineralizer is aluminum phosphate or magnesium pyrophosphate. The reduction sintering process involves first heating the temperature to 750-850°C in a reducing atmosphere and holding the temperature for 20-30 minutes; then heating the temperature to 1100-1250°C and melting the mixture for 25-50 minutes, and finally quenching the melt. The main raw materials of the present invention are all waste glass, which reduces the cost of the raw materials and produces ceramic frits with high thermal stability.

The present invention relates to a method for target nucleic acid detection using a loop-mediated isothermal amplification (LAMP) technique. In order to solve the problem of high cost, complexity, or low specificity of conventional LAMP detection methods, the present invention confirms that magnesium pyrophosphate, which is a byproduct of a LAMP reaction, is produced as microparticles having unique morphological characteristics under a specific condition, and provides a new detection principle for directly identifying spherical microparticles having an average diameter in the range of 0.5-2.0 micrometers present in a reaction mixture by using a high-magnification (400x or higher) optical device. Through such morphological analysis, false positive signals resulting from non-specific amplification may be effectively excluded, and whether nucleic acid amplification occurs may be determined with high reliability.