4 results about "Magnesium pyrophosphate" patented technology

The invention relates to a sliding material based on PTFE polymer with a filler to improve the friction properties, wherein the filler comprises at least one phosphate, in particular calcium phosphate, calcium pyrophosphate, magnesium phosphate, magnesium pyrophosphate, lithium phosphate, hydroxyapatite or combinations thereof, and at least one metal sulfide, wherein the proportion of metal sulfide is > 2%. The invention also relates to the use of the sliding material.

The application discloses a dye-free naked-eye recognizable nucleic acid culture detection counting method, and belongs to the technical field of digital nucleic acid culture. The method comprises the following steps: (1) adding water gel monomers acrylamide and methylene bisacrylamide into a loop-mediated isothermal amplification reaction system of a to-be-detected sample, and initiating crosslinking to form a water gel system; (2) isothermal amplification reaction; (3) after the amplification is completed, a magnesium pyrophosphate precipitate point formed in the water gel system is recognized and counted, and the concentration of target nucleic acid molecules in the to-be-detected sample is calculated. The application combines polyacrylamide water gel and digital LAMP, utilizes the adsorption flocculation capacity of PAM to control the LAMP reaction by-product magnesium pyrophosphate precipitation in the vicinity of a nucleic acid amplification group, forms a white point which has a clear color difference with a background, and the position and size of the white point are basically consistent with those of the nucleic acid amplification group, and thus the dye-free naked-eye recognizable nucleic acid culture counting effect is realized.

The invention provides a preparation method of magnesium pyrophosphate coated magnesium oxide. The preparation method comprises the following steps: firstly, sequentially carrying out ball-milling pretreatment on magnesium oxide, an ethanol-water mixed solution and a silane coupling agent, and forming a magnesium hydroxide layer and a silane hydrophobic protection layer on the surface of magnesium oxide to obtain pre-hydrolyzed magnesium oxide particles; and then mixing and ball-milling with a phosphorus-containing mineralizing agent, and carrying out high-temperature melting and calcining, so that the mineralizing agent reacts with the magnesium hydroxide layer to generate a compact magnesium pyrophosphate coating layer, namely the magnesium pyrophosphate-coated magnesium oxide is prepared. According to the preparation method disclosed by the invention, effective coating of magnesium pyrophosphate on magnesium oxide is successfully realized through a simple ball-milling and calcining process, the hydrolysis resistance is remarkably improved, and meanwhile, the obtained powder is good in dispersity and uniform in particle size, and has a wide application prospect in the field of high-thermal-conductivity composite materials.

The present invention relates to a method for target nucleic acid detection using a loop-mediated isothermal amplification (LAMP) technique. In order to solve the problem of high cost, complexity, or low specificity of conventional LAMP detection methods, the present invention confirms that magnesium pyrophosphate, which is a byproduct of a LAMP reaction, is produced as microparticles having unique morphological characteristics under a specific condition, and provides a new detection principle for directly identifying spherical microparticles having an average diameter in the range of 0.5-2.0 micrometers present in a reaction mixture by using a high-magnification (400x or higher) optical device. Through such morphological analysis, false positive signals resulting from non-specific amplification may be effectively excluded, and whether nucleic acid amplification occurs may be determined with high reliability.