37 results about "Magnesium pyrophosphate" patented technology

The invention discloses a kit and oligonucleotide sequences for detecting rotavirus A, in particular to a group of oligonucleotides which are used for detecting rotavirus A and have the oligonucleotide sequences shown from the sequence table SEQ ID No.1 to the sequence table SEQ ID No.5, the kit containing the oligonucleotides and a detection method thereof. The kit of the invention has high sensitivity, strong specificity, low cost and simple and convenient operation. During detection, when lots of nucleic acid is synthesized, a by-product magnesium pyrophosphate precipitate is generated, which has high specificity. Whether the products are amplified or not can be judged only by observing the turbidity of the products with naked eyes.

The invention provides a gene fast diagnostic reagent kit for Mycobacterium tuberculosis based on a loop-mediated isothermal amplification technology and a detection method thereof, wherein, the reagent kit consists of two pairs of primers, DNA polymerase, reaction liquid, lysate 1, lysate 2, stabilizing liquid, chromogenic solution and positive control liquid, and the eight liquids are respectively placed in containers. The gene fast diagnostic reagent kit uses six sections and four primers, and can judge whether a target substance exists according to the condition of the augmentation, so the gene fast diagnostic reagent kit has high specificity; the gene fast diagnostic reagent kit is quick and efficient, has high sensitivity, can perform the augmentation reaction only with a constant temperature, and does not need special reagent and equipment; the gene fast diagnostic reagent kit has simple and convenient identification, pyrophosphate ions precipitated from dNTP are combined with Mg<2+> in reaction solution to produce a byproduct, namely magnesium pyrophosphate precipitate, the reaction can be identified through the observation of naked eyes, and positive and negative results have a remarkable chromogenic difference when the chromogenic solution is added, thereby being more apparent and reliable.

A product which contains acid magnesium pyrophosphate (magnesium dihydrogen diphosphate) and at least magnesium orthophosphate, producible by removal of water from an aqueous solution or suspension of a magnesium phosphate compound which has a molar ratio of Mg:P of 0.4-0.6:I, wherein the product has a loss on ignition of 7.5 to 13.5% and its method of preparation and its use in a leavening agent for bakery products.

The invention discloses establishment of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) detection method for Prunus necrotic ringspot virus (PNRSV). According to the RT-LAMP detection method for the PNRSV, four specific primers are designed for six regions of a target gene PNRSV, a type of strand displacement deoxyribonucleic acid (DNA) polymerase is utilized at the constant temperature of around 65 DEG C, and efficient amplification of nucleic acids can be achieved in dozens of minutes. The four specific primers are used for identifying the six specific sequence regions of a target sequence, and therefore high specificity of amplification of LAMP is guaranteed. In the process of LAMP, thermal denaturation of template is not needed, temperature is cycled for a long time, the amplification is carried out under isothermal conditions, a waste of time due to temperature change is not caused, and the reaction speed can be increased by 30-50%. Based on whether visible white precipitate of magnesium pyrophosphate exists in a reaction tube, whether the nucleic acids are amplified can be easily judged. The RT-LAMP method for detection of the PNRSV is high in specificity, and the RT-LAMP method for detection of the PNRSV has the advantages of being rapid, high in efficiency, simple in instrument requirement, simple and convenient to operate, low in cost and easy to popularize and use at the grass-roots.

The invention discloses a rapid diagnostic reagent kit of legionella pneumophilia genes based on a loop-mediated isothermal amplification technique and a detecting method thereof, wherein the reagentkit consists of two pairs of primers, DNA polymerase, a stable liquid, a reaction liquid, a sample pretreatment liquid, a color rendering liquid and a positive contrast liquid which are respectively placed in a container. The gene rapid diagnostic reagent kit can judge whether target substances exist or not by applying applies six sections and the four primers according amplification or non-amplification, and thereby has high specificity. The gene rapid diagnostic reagent kit has high speed, high efficiency and high sensitivity, only needs a constant temperature for amplification reaction without special reagents and equipment, and has low detection cost. The gene rapid diagnostic reagent kit has simple identification, can generate magnesium pyrophosphate deposits as a byproduct by combining pyrophosphate ions precipitated from dNTP and Mg<2+> in a reaction solution, can identify the magnesium pyrophosphate deposits through visual study, and has remarkable color rendering difference ofnegative and positive results after the color rendering liquid is added, and is more marked and reliable.

The invention discloses a rapid diagnosis kit and a detection method of genes of comma bacillus. The kit consists of two pairs of primers, DNA polymerase, stabilizing solution, reaction solution, sample pre-treatment solution, color development solution and positive comparison solution, wherein, the seven solutions are respectively placed in containers. The rapid diagnosis kit of genes of the invention applies six sections and four primers and can judge whether target materials exist only according to the amplification, thus being high in specificity. The rapid diagnosis kit of genes of the invention is high in speed, high in efficiency and sensitivity, can amplify the reaction with only constant temperature, without special reagents or equipment, and is low in detection cost. The rapid diagnosis kit of genes of the invention is simple and convenient in identification. Pyrophosphate ions separated out from the dNTP are combined with Mg<2+> in the reaction solution to produce a by-product which is the sediment of magnesium pyrophosphate which can be identified by naked eyes. Furthermore, after the color development solution is added, the color development differences of negative and positive results are notable and the identification is more obvious and reliable.

The invention discloses a primer group and a kit for detecting Roundup Ready transgenic soybeans. The kit consists of the primer group, Bst deoxyribonucleic acid (DNA) polymerase, a stabilizing solution, a reaction solution, a developing solution and a positive control solution. In the kit, six segments and four primers are utilized; and the kit has high specificity according to the condition that whether the existence of target materials can be judged or not through amplification. The quick diagnostic kit provided by the invention has high speed, high efficiency and high sensitivity, can be subjected to an amplification reaction only by a constant temperature without the usage of special reagents and equipment, has low detection cost and is easy and convenient to identify; pyrophosphate ions precipitated from deoxyribonucleoside triphosphate (dNTP) is combined with Mg<2+> in the reaction solution; the produced byproduct, namely magnesium pyrophosphate is precipitated and can be observed and identified through naked eyes; and after the developing solution is added, the kit has obvious development differences of negative and positive results and is more obvious and reliable. A soybean endogenous gene lectin is used as an internal label, so that errors and false negatives caused by the extraction of nucleic acid are prevented; and real-time monitoring is performed, so that detection time is greatly reduced, and human power and material resources are saved.

The invention provides a silver-containing inorganic antibacterial agent with excellent transparency. The silver-containing inorganic antibacterial agent with excellent transparency is a silver-containing inorganic antibacterial agent which contains magnesium pyrophosphate and is represented by a formula AgaMbMgcRd(PO4)2.NH2O, wherein M is at least one selected from alkali metal ions, ammonium ions, hydrogen ions and oxonium ions, R is a metal ion having a valence of 2, a, b, c, d and n are 3 or less, a and c are positive numbers, 2.5 < (c + d) < 3 and a + b + 2(c + d) = 6. The silver-containing inorganic antibacterial agent disclosed by the invention has the characteristics of excellent heat resistance, discoloration resistance, washing resistance and processability when being applied tohigh polymer materials, and particularly can be applied to transparent products.

The invention provides a mononuclear hyperplasia Listeria monocytogenes gene quick diagnosis reagent box based on a loop-mediated isothermal amplification technology and a method for detecting the same. The reagent box consists of two pairs of primers, DNA polymerase, reaction liquid, sample pretreatment liquid, developing liquid and a positive contrast solution, and the six kinds of liquid are respectively contained in a container. The gene quick diagnosis reagent box adopts six segments and four primers, and can judge whether a target substance exists or not according to the fact whether amplification occurs or not, thereby having high specificity. Moreover, the reagent box has quickness, high efficiency and high sensitivity, and can carry out amplification reaction just at a constant temperature without adopting special reagent and equipment. In addition, the reagent box can realize simple identification; therefore, pyrophosphate radical ion separated out from dNTP is combined with Mg<2+> in a reaction solution so as to generate byproduct magnesium pyrophosphate precipitate which can be identified through unaided viewing; moreover, after the developing liquid is added, the remarkable positive and negative result color developing difference is more obvious and reliable.

The invention discloses a rapid diagnosis kit and a detection method of genes of staphylococcus epidermidis. The kit consists of two pairs of primers, DNA polymerase, stabilizing solution, reaction solution, sample pre-treatment solution, color development solution and positive comparison solution, wherein, the seven solutions are respectively placed in containers. The rapid diagnosis kit of genes of the invention applies six sections and four primers and can judge whether target materials exist only according to the amplification, thus being high in specificity. The rapid diagnosis kit of genes of the invention is high in speed, high in efficiency and sensitivity, can amplify the reaction with only constant temperature, without special reagents or equipment, and is low in detection cost. The rapid diagnosis kit of genes of the invention is simple and convenient in identification. Pyrophosphate ions separated out from the dNTP are combined with Mg<2+> in the reaction solution to produce a by-product which is the sediment of magnesium pyrophosphate which can be identified by naked eyes. Furthermore, after the color development solution is added, the color development differences of negative and positive results are notable and the identification is more obvious and reliable.

The invention discloses a golden yellow staphylococcus gene quick diagnosis reagent box and a method for detecting the same. The reagent box consists of two pairs of primers, DNA polymerase, reaction liquid, sample pretreatment liquid, developing liquid and a positive contrast solution, and the six kinds of liquid are respectively contained in a container. The gene quick diagnosis reagent box adopts six segments and four primers, and can judge whether a target substance exists or not according to the fact whether amplification occurs or not, thereby having high specificity. Moreover, the reagent box has quickness, high efficiency and high sensitivity, and can carry out amplification reaction just at a constant temperature without adopting special reagent and equipment, thereby ensuring low detection cost. In addition, the reagent box can realize simple identification; therefore, pyrophosphate radical ion separated out from dNTP is combined with Mg<2+> in a reaction solution so as to generate byproduct magnesium pyrophosphate precipitate which can be identified through unaided viewing; moreover, after the developing liquid is added, the remarkable positive and negative result color developing difference is more obvious and reliable.

The invention discloses a rapid diagnosis kit and a detection method of genes of campylobacter jejuni. The kit consists of DNA polymerase, a stabilizing solution, a reaction solution, a sample pre-treatment solution, a color development solution and a positive comparison solution, wherein, the five solutions are respectively placed in containers. The rapid diagnosis kit of genes of the invention applies six sections and four primers and can judge whether target materials exist only according to the amplification, thus being high in specificity. The rapid diagnosis kit of genes of the invention is high in speed, high in efficiency and sensitivity, can amplify the reaction with only constant temperature, without special reagents or equipment, and is low in detection cost. The rapid diagnosis kit of genes of the invention is simple and convenient in identification. Pyrophosphate ions separated out from the dNTP are combined with Mg<2+> in the reaction solution to produce a by-product which is the sediment of magnesium pyrophosphate which can be identified by naked eyes. Furthermore, after the color development solution is added, the color development differences of negative and positive results are notable and the identification is more obvious and reliable.

Colored CTE modifiers may be added to a glass frit system to modify the CTE of a resulting fired enamel. The CTE modifier is colored. The colored CTE modifier may include a modified Pseudo-Brookite type material having a formula Al₂TiO₅, where Al and/or Ti are partially substituted with one or more coloring ions including Fe, Cr, Mn, Co, Ni, and Cu; a modified Cordierite type material having a formula Mg₂Al₄Si₅O₁₈, wherein Mg and/or Al is partially substituted with one or more of the coloring ions; a Perovskite type material having a formula Sm_1−xSr_xMnO_3−δ, where x=0.0-0.5 and δ=0.0-0.25, or a modified version of the Perovskite type material wherein Sr is partially substituted with Ba and/or Ca; a modified magnesium pyrophosphate type material having a formula Mg₂P₂O₇ wherein Mg is substituted with Co and/or Zn ions; or combinations thereof.