9results about How to "Highly specific" patented technology

The present invention belongs to the technical field of surface enhanced Raman scattering, and in particular, is a noble metal SERS substrate for detecting specific nerve agents and a preparation method thereof. The substrate of the present invention is composed of a nanostructured noble metal sensitive layer with a Raman enhancement effect and a capture agent molecule, wherein the capture agent molecule includes an anchoring functional group that forms a stable bond with the nanostructured noble metal sensitive layer, an active functional group with a Raman scattering cross section, and a capture functional group that forms a chemical bond with the nerve agent. The present invention highly selectively captures trace amounts of organic phosphine nerve agent molecules in a complex environment by modifying the capture agent molecules with multiple functional groups on the surface of the noble metal substrate, thereby achieving trace detection.

The application provides a high-expression molecular marker 5'-tiRNA for gastric cancer screening Gly , and relates to the technical field of biomedicine. The application provides a high-expression molecular marker for gastric cancer screening, characterized in that the molecular marker is 5'-tiRNA Gly , and the 5'-tiRNA Gly is derived from the 5'-half of tRNA-Gly-GCC. The application proves that the 5'-tiRNA Gly can be used as a gastric cancer diagnosis marker, and can be used for diagnosing and predicting gastric cancer by detecting the expression amount of the 5'-tiRNA Gly in serum, and has the advantages of high specificity and sensitivity.

The invention relates to the technical field of nano-materials, in particular to a polypeptide nano-material for binding HER2 and a preparation method and application thereof, the polypeptide nano-material comprises polypeptide with an amino acid sequence as shown in SEQ ID NO: 1 and an I-type photosensitizer connected with the last amino acid of the polypeptide; the polypeptide comprises a ligand unit and a fiber deformation element; the I-type photosensitizer is connected to one end, deviating from the ligand unit, of the fiber deformation element, and the I-type photosensitizer has the aggregation-induced emission characteristic. The polypeptide nano-material can be self-assembled in an aqueous solution through hydrophilic and hydrophobic effects to form spherical nano-particles, the spherical nano-particles are disintegrated through ligand-receptor interaction induction, and an ordered, stable and continuous beta-folding structure is formed through a pi-pi accumulation effect and a hydrogen bond, so that the polypeptide nano-material is converted into a nanofiber network structure in situ in a tumor, and the tumor activity is improved. The type and intensity of active oxygen generated by the I-type photosensitizer with AIE characteristics can be remarkably improved, and finally, specific efficient photodynamic therapy of HER2 positive breast cancer is realized.

The present invention relates to an antigen-binding protein, antibody or antibody active fragment obtained by immunizing camelids with Rab8 protein. The present invention relies on the immune system of camelids to screen, identify and prepare antibodies that specifically recognize and bind to Rab8 protein. The obtained antibodies have strong specificity and can be used for target antigen detection, with potential clinical diagnosis and treatment value; and the antibody provided by the present invention has a simple structure, is easy to be genetically engineered, is easy to be humanized, has high stability, and has low mass production cost, which is conducive to large-scale production.

This invention discloses a metal nanoparticle reagent kit, its preparation method, and its application in the detection of eczema biomarkers. The metal nanoparticle reagent kit utilizes a chemical self-assembly method to deposit metal nanoparticles onto the surface of the kit's well plates. ADNA is modified onto the surface of the metal nanoparticles through Ag-S bonds. Subsequently, through a complementary pairing process between the ADNA and cDNA chains, a cDNA chain with a Cy3 terminus at one end is brought close to the metal nanoparticles to synthesize a nucleic acid aptamer sensor. The metal nanoparticle reagent kit can specifically recognize and rapidly capture eczema biomarkers using nucleic acid aptamers. Combined with surface-enhanced Raman spectroscopy, it achieves rapid, sensitive, and accurate qualitative and quantitative detection of eczema biomarkers. Simultaneously, the internally encapsulated internal standard molecule signal can dynamically correct the Raman signal in the metal nanoparticle reagent kit.

The invention relates to the technical field of biological detection, in particular to a nucleic acid composition, a detection system and a detection device for rapidly detecting klebsiella pneumoniae and carbapenem drug resistance genes. The invention relates to a nucleic acid composition for rapidly detecting klebsiella pneumoniae and carbapenem drug resistance genes. The nucleic acid composition comprises a one-step detection primer group combining isothermal amplification and CRISPR (clustered regularly interspaced short palindromic repeats), the invention discloses a detection system for rapidly detecting klebsiella pneumoniae and carbapenem drug resistance genes. The detection system comprises a nucleic acid composition, a loop-mediated isothermal amplification reaction reagent, a CRISP reaction reagent and an indicator, a detection device for rapidly detecting klebsiella pneumoniae and carbapenem drug resistance genes is characterized by comprising a mixing plate and a reaction plate, reaction is performed under the constant temperature condition, a reaction carrier is easy to obtain, a temperature cycle instrument which is high in value and tedious in step is not needed, and the detection cost is reduced. The reaction time is greatly shortened, and the detection efficiency is improved.

The invention discloses a cyanide ratio response Raman probe and a preparation method and application thereof.The probe is composed of a gold nanostar substrate and cyanide ion response Raman reporter molecules connected through gold-sulfur bonds, and the reporter molecules are connected with 1, 1, 2, 3, 4-tetracarboxylic acid through ethylene bridges. 2, 2-trimethyl-3-ethyl-1H-benzoindole and N-ethylcarbazole-3-formaldehyde are used as recognition units, and can specifically react with CN <->. The probe adopts a ratio detection strategy, takes a 1507cm <-1 > peak as an internal reference and a 2105cm <-1 > peak as a response signal, is linearly correlated with the CN <-> concentration in a range of 0.1-10 mu M (R2 is equal to 0.9902), the detection limit reaches 0.1 mu M, and the sensitivity is improved by more than 10 times compared with that of a traditional SERS (Surface Enhanced Raman Scattering) method. The probe is high in anti-interference performance, response to common anions, thiol and active oxygen can be ignored, signal change to CN <-> reaches 15.5 times, single detection only needs 3 minutes, and the probe is suitable for rapid and high-selectivity quantitative detection of cyanide in a complex environment.

Provided are a recombinant yeast for producing lacto-N-triose, and an application thereof, belonging to the bioengineering field. The recombinant yeast comprises an amino acid sequence of a β-N-acetylglucosaminyl transferase and a coding nucleic acid sequence thereof, and / or a disruption of a marker gene. The technical solution of the present invention has the advantages of easy availability of raw materials, simple and feasible method, suitable for industrial production, and excellent application prospects.

The invention relates to the technical field of microorganisms, in particular to a penicillium oxalicum strain and preparation and application of a fermentation crude extract of the penicillium oxalicum strain, penicillium oxalicum D10 is obtained from mangrove forest rhizosphere soil, and detection shows that the fermentation crude extract of the penicillium oxalicum strain has a strong bacteriostatic effect on key vibrio pathogens. The minimum inhibitory concentrations (MIC) of the compound on vibrio parahaemolyticus, vibrio frenesii and vibrio alginolyticus are respectively 31.25 [mu] g / mL, 7.81 [mu] g / mL and 0.49 [mu] g / mL through determination by a trace double dilution method. The activity of the crude extract on vibrio alginolyticus is particularly prominent, more obvious high efficiency is achieved, active substances of the crude extract possibly derive from novel secondary metabolites and are different from an existing antibacterial mechanism, the problem of drug resistance is expected to be overcome, and high-quality germplasm resources are provided for later preparation of microecological preparation products.