6results about How to "Highly specific" patented technology

The application provides a high-expression molecular marker 5'-tiRNA for gastric cancer screening Gly , and relates to the technical field of biomedicine. The application provides a high-expression molecular marker for gastric cancer screening, characterized in that the molecular marker is 5'-tiRNA Gly , and the 5'-tiRNA Gly is derived from the 5'-half of tRNA-Gly-GCC. The application proves that the 5'-tiRNA Gly can be used as a gastric cancer diagnosis marker, and can be used for diagnosing and predicting gastric cancer by detecting the expression amount of the 5'-tiRNA Gly in serum, and has the advantages of high specificity and sensitivity.

The invention relates to the technical field of nano-materials, in particular to a polypeptide nano-material for binding HER2 and a preparation method and application thereof, the polypeptide nano-material comprises polypeptide with an amino acid sequence as shown in SEQ ID NO: 1 and an I-type photosensitizer connected with the last amino acid of the polypeptide; the polypeptide comprises a ligand unit and a fiber deformation element; the I-type photosensitizer is connected to one end, deviating from the ligand unit, of the fiber deformation element, and the I-type photosensitizer has the aggregation-induced emission characteristic. The polypeptide nano-material can be self-assembled in an aqueous solution through hydrophilic and hydrophobic effects to form spherical nano-particles, the spherical nano-particles are disintegrated through ligand-receptor interaction induction, and an ordered, stable and continuous beta-folding structure is formed through a pi-pi accumulation effect and a hydrogen bond, so that the polypeptide nano-material is converted into a nanofiber network structure in situ in a tumor, and the tumor activity is improved. The type and intensity of active oxygen generated by the I-type photosensitizer with AIE characteristics can be remarkably improved, and finally, specific efficient photodynamic therapy of HER2 positive breast cancer is realized.

This invention discloses a metal nanoparticle reagent kit, its preparation method, and its application in the detection of eczema biomarkers. The metal nanoparticle reagent kit utilizes a chemical self-assembly method to deposit metal nanoparticles onto the surface of the kit's well plates. ADNA is modified onto the surface of the metal nanoparticles through Ag-S bonds. Subsequently, through a complementary pairing process between the ADNA and cDNA chains, a cDNA chain with a Cy3 terminus at one end is brought close to the metal nanoparticles to synthesize a nucleic acid aptamer sensor. The metal nanoparticle reagent kit can specifically recognize and rapidly capture eczema biomarkers using nucleic acid aptamers. Combined with surface-enhanced Raman spectroscopy, it achieves rapid, sensitive, and accurate qualitative and quantitative detection of eczema biomarkers. Simultaneously, the internally encapsulated internal standard molecule signal can dynamically correct the Raman signal in the metal nanoparticle reagent kit.

The invention discloses a cyanide ratio response Raman probe and a preparation method and application thereof.The probe is composed of a gold nanostar substrate and cyanide ion response Raman reporter molecules connected through gold-sulfur bonds, and the reporter molecules are connected with 1, 1, 2, 3, 4-tetracarboxylic acid through ethylene bridges. 2, 2-trimethyl-3-ethyl-1H-benzoindole and N-ethylcarbazole-3-formaldehyde are used as recognition units, and can specifically react with CN <->. The probe adopts a ratio detection strategy, takes a 1507cm <-1 > peak as an internal reference and a 2105cm <-1 > peak as a response signal, is linearly correlated with the CN <-> concentration in a range of 0.1-10 mu M (R2 is equal to 0.9902), the detection limit reaches 0.1 mu M, and the sensitivity is improved by more than 10 times compared with that of a traditional SERS (Surface Enhanced Raman Scattering) method. The probe is high in anti-interference performance, response to common anions, thiol and active oxygen can be ignored, signal change to CN <-> reaches 15.5 times, single detection only needs 3 minutes, and the probe is suitable for rapid and high-selectivity quantitative detection of cyanide in a complex environment.

Provided are a recombinant yeast for producing lacto-N-triose, and an application thereof, belonging to the bioengineering field. The recombinant yeast comprises an amino acid sequence of a β-N-acetylglucosaminyl transferase and a coding nucleic acid sequence thereof, and / or a disruption of a marker gene. The technical solution of the present invention has the advantages of easy availability of raw materials, simple and feasible method, suitable for industrial production, and excellent application prospects.

The invention relates to the technical field of microorganisms, in particular to a penicillium oxalicum strain and preparation and application of a fermentation crude extract of the penicillium oxalicum strain, penicillium oxalicum D10 is obtained from mangrove forest rhizosphere soil, and detection shows that the fermentation crude extract of the penicillium oxalicum strain has a strong bacteriostatic effect on key vibrio pathogens. The minimum inhibitory concentrations (MIC) of the compound on vibrio parahaemolyticus, vibrio frenesii and vibrio alginolyticus are respectively 31.25 [mu] g / mL, 7.81 [mu] g / mL and 0.49 [mu] g / mL through determination by a trace double dilution method. The activity of the crude extract on vibrio alginolyticus is particularly prominent, more obvious high efficiency is achieved, active substances of the crude extract possibly derive from novel secondary metabolites and are different from an existing antibacterial mechanism, the problem of drug resistance is expected to be overcome, and high-quality germplasm resources are provided for later preparation of microecological preparation products.