6 results about "Deoxyribonucleotide Triphosphate" patented technology

The application relates to the field of organic membrane separation, and specifically discloses a method for replacing deoxyribonucleotide triphosphate amine salt with sodium salt by adopting nanofiltration roll type membrane. The method specifically comprises the following steps: a concentration step, concentrating deoxyribonucleotide triphosphate amine salt fraction after high-pressure liquid phase purification; a replacement step, adding 1.5mol / L-6mol / L sodium (potassium) carbonate or sodium (potassium) hydrogen carbonate 0.5V-2V salt solution to the deoxyribonucleotide triphosphate amine salt solution for low-temperature replacement; and a desalination step, introducing carbon dioxide into the system in the above step, and washing with water until sodium ions cannot be detected in the filtered liquid, so as to obtain a deoxyribonucleotide triphosphate sodium salt solution. The preparation method can replace the organic amine salt with sodium salt, and 100% replacement of the organic amine salt can be realized.

The invention provides a DNA paper-cutting system, method and product for resisting polymerase chain displacement, and belongs to the field of DNA nanotechnology, the DNA paper-cutting system comprises: a DNA nanostructure comprising a cutting region and a non-cutting region; wherein on the template chain of the non-cutting area, a blocking sequence is arranged at the 5'end of a template chain section corresponding to at least one nicking site, and the blocking sequence is composed of one or more basic groups belonging to a target basic group type; the specific reaction buffer solution contains DNA (deoxyribonucleic acid) polymerase and a plurality of deoxyribonucleoside triphosphates except a specific deoxyribonucleoside triphosphate; wherein the type of the specific deoxyribonucleoside triphosphate deleted in the specific reaction buffer solution is complementary to the type of the target basic group.

This invention discloses a method for detecting B7-H3 protein based on signal conversion and multiplex isothermal amplification of nucleic acids. The detection method involves adding the sample to be tested, an anti-B7-H3 antibody-DNA1 / DNA3 complex, an anti-B7-H3 antibody-DNA2 conjugate, a hairpin probe assembly, deoxyribonucleotide triphosphate, nicking endonuclease, DNA polymerase, and a fluorescent dye to a detection system buffer. After reaction, fluorescence detection is performed. This method enables rapid detection of B7-H3 protein. It uses fluorescence as the detection signal, exhibits high specificity and sensitivity, and achieves a single-tube, one-step detection method with only one sample addition, eliminating the need for opening the tube for cleaning.

This invention relates to methods for amplifying target nucleic acid. A target nucleic acid is contacted with specific primers in the presence of DNA polymerase and deoxyribonucleotide triphosphates to produce an amplification solution with a volume of 5 pl or less. The amplification solution is subjected to a denaturation temperature of 75° C.-85° C. for 1.5 second or less; and then to a polymerisation temperature of 25° C.-45° C. for 1 second or less. These denaturation and polymerisation steps are repeated to amplify the target nucleic acid. Devices and systems for performing such methods are also provided.

The disclosure herein includes methods and compositions for nucleic acid synthesis using terminal deoxynucleotidyl transferase with a plurality of deoxyribonucleotide triphosphates each comprising a modified base having a photocleavable carbon chain moiety, which when present, enables single incorporation.