2 results about "Deoxyribonucleotide Triphosphate" patented technology

The application belongs to the technical field of sequencing-by-synthesis chip, and particularly relates to a three-dimensional porous array enhanced SBS sequencing chip and a preparation method thereof, which comprises a silicon wafer as a substrate and a light-controlled reversible terminator reaction system; the silicon wafer surface has a mesoporous film, and the mesoporous film has reaction cavities and a closed microchannel network; the inner wall of the mesoporous film is grafted with mercaptopropionic acid and covalently coupled with an amino-modified probe; the reaction cavity bottom is etched with a cylindrical array; the light-controlled reversible terminator reaction system comprises deoxyribonucleotide triphosphates, the 3'-OH end of the deoxyribonucleotide triphosphates is introduced with a protective group, and the 5' end amino group of the deoxyribonucleotide triphosphates is specifically combined with a fluorescent group; and the application effectively breaks through the technical bottlenecks of the existing NGS sequencing chip in terms of throughput, accuracy, cost and adaptability of complex samples.

The disclosure herein includes methods and compositions for nucleic acid synthesis using terminal deoxynucleotidyl transferase with a plurality of deoxyribonucleotide triphosphates each comprising a modified base having a photocleavable carbon chain moiety, which when present, enables single incorporation.