14 results about "Processing enzymes" patented technology

Feedstock including an amphiphilic lignocellulosic biomass can be processed using enzymes prior to pulping. The enzymes break down the biomass by disrupting the surface or cell walls or by reducing wax or other components. Such enzymes include cellulases, hemicellulases, laccases, peroxidases, and pectinases. Suitable enzymes can be obtained from secretions of microbes in soil organic matter from soil in which the feedstock was grown. If the pulping process includes steam explosion, such enzy matic processing of a biomass prior to steam explosion will increase absorption of water by the processed feedstock. In steam explosion, due to the increased ability of the processed feedstock to absorb water, steam explosion is more effective in breaking apart the processed feedstock. For other pulping processes, such enzymatic treatments break down the biomass, enabling extraction of the desired components from the processed feedstock through subsequent processing using less energy and fewer chemicals.

The application discloses RhVPE1 protein and application of a coding gene thereof in regulation and delay of cell programmed death of Chinese rose petal senescence, and an amino acid sequence of the RhVPE1 protein is shown as SEQ ID NO. 3. The application clones a gamma-VPE vacuole processing enzyme RhVPE1 gene from Chinese rose petal, finds that expression of the RhVPE1 gradually rises with petal senescence, and is induced by ethylene, and that the speed of petal from the 5th grade to losing ornamental value is slowed down by silencing of the RhVPE1 gene. In the petal with the RhVPE1 silenced, expression of an aging indicating gene SAG12 is significantly down-regulated. The research result of the application reveals the role of the RhVPE1 in ethylene-regulated petal cell programmed death and senescence, provides a new thought and technical means for regulating plant flower senescence, especially postharvest preservation of Chinese rose fresh-cut flowers, opens up a new flower regulation research field, and has great academic value and important production application prospect.

Provided is a novel technique for improving the taste quality of a glycosylated steviol glycoside composition. In the present technology, provided are a method for producing a glycosylated steviol glycoside composition and a method for improving the taste quality of a glycosylated steviol glycoside composition, each of which comprises a step in which a cyclodextrin glycosyltransferase derived from a microorganism belonging to the genus anaerobic bacillus is caused to act on a stevia extract and dextrin. In the production method and the taste quality improvement method according to the present technology, a step for causing a saccharide processing enzyme different from the cyclodextrin glycosyltransferase derived from the microorganism belonging to the genus anaerobic bacillus to act can be further performed.

Feedstock including an amphiphilic lignocellulosic biomass can be processed using enzymes prior to pulping. The enzymes break down the biomass by disrupting the surface or cell walls or by reducing wax or other components. Such enzymes include cellulases, hemicellulases, laccases, peroxidases, and pectinases. Suitable enzymes can be obtained from secretions of microbes in soil organic matter from soil in which the feedstock was grown. If the pulping process includes steam explosion, such enzy matic processing of a biomass prior to steam explosion will increase absorption of water by the processed feedstock. In steam explosion, due to the increased ability of the processed feedstock to absorb water, steam explosion is more effective in breaking apart the processed feedstock. For other pulping processes, such enzymatic treatments break down the biomass, enabling extraction of the desired components from the processed feedstock through subsequent processing using less energy and fewer chemicals.

The invention discloses a multi-filtering device and method for enzyme processing, and relates to the technical field of enzyme processing. A feed port; a filter funnel; a conical flow guide block; a blocking deslagging piece; and the gap swaying part is arranged at the top of the filtering funnel and is used for increasing the flowability of the filtered impurities on the inner side of the filtering funnel. By arranging the blocking and deslagging piece and rotating a rotating connecting shaft, the rotating connecting shaft rotates by 180 degrees relative to the tank body, and when the rotating connecting shaft rotates by 90 degrees, a first blocking block moves to the inner side of a material storage pipe, so that the first blocking block is located above a second blocking block; when the rotating connecting shaft continues to rotate by 90 degrees, the second blocking block retracts into the inner side of the first storage bin, so that the impurities above the second blocking block are not blocked, the impurities on the inner side of the material storage pipe are discharged along the slag discharging pipe at the moment, and therefore the filtered impurities can be cleaned under the condition that equipment operation is not affected; the filtering efficiency of the equipment is further improved.

The invention provides a GlycoDepth method for remarkably improving the identification depth of a glycoprotein group by utilizing a CRISPR / Cas9 (clustered regularly interspaced short palindromic repeats / CRISPR associated protein 9) gene editing technology and an inhibitor treatment material and combining site-specific N-glycoprotein omics analysis. According to the method, enrichment of specific glycoforms in plant materials such as tomatoes is realized through targeted knockout or inhibition of specific glycosyl processing enzymes, so that heterogeneity of glycopeptides is reduced, and the sensitivity of mass spectrometric detection and the identification depth of N-glycoprotein groups are improved. The invention also provides application of the method in glycoprotein function research, crop quality improvement and sugar engineering.

The invention provides a novel method for performing injection treatment on fleshy fruits by using an N-glycosylation processing enzyme inhibitor and researching the influence of the enzyme on fruit maturation through phenotype and protein glycotype changes. As the tomato is rich in nutrition, short in growth cycle, small in genome and mature in genetic transformation system, the tomato becomes an excellent model plant for researching fleshy fruits; the method specifically comprises the following steps: marking flowers after flowering of tomatoes, selecting fruits 17-24 days after flowering, injecting an inhibitor with the concentration of 1-10 M into a fruit navel part for 1-3 times, injecting 10-100 times every time, injecting 5-10 days every time, and taking injection water as a contrast; the reduced N-sugar chain is detected by combining SA / 2HTA. Results show that fruits of a treatment group turn color about 5 days earlier than that of a control group, softening and ethylene release are accelerated, fruit stems fall off 5 days after color turning, seeds are immature, and the germination rate is 0%; detection finds that the content of Man9GlcNAc2 is remarkably increased, and the efficient feasibility of screening the key N-glycosylation processing enzyme for fruit regulation and control through an inhibitor-phenotype-protein glycotype integrated platform is proved.

The embodiment of the application discloses a kind of prediction model training method, computer equipment and computer storage medium.The joint learning of amino acid sequence and molecular information sequence of two " languages " is carried out in pre-training phase, and the parameter optimization of enzyme-substrate interaction judgment task is carried out in fine-tuning phase, and the prediction model constructed by the method can naturally fuse the amino acid sequence characteristics of enzyme and the molecular information sequence characteristics of substrate.The model is no longer isolatedly analyzed enzyme or substrate when processing enzyme-substrate pair, but the two are jointly modeled as an organic whole.This cross-modal feature fusion capability enables the model to more deeply understand the nature mechanism of enzyme-substrate interaction, thereby effectively breaking through the limitation of " single language " model when processing heterogeneous data, significantly improving the generalization ability and prediction accuracy in the enzyme-substrate interaction prediction task of cross enzyme family or far evolutionary distance.

The invention discloses an automatic filling machine for producing a food processing enzyme preparation and a method of the automatic filling machine, and belongs to the technical field of filling machines. The conveying assembly comprises a fixed conveying assembly, an adjustable conveying assembly, an electronic weighing instrument, a horizontal direction adjusting assembly, a clamping assembly and a roller type conveying assembly, the electronic weighing instrument is connected with the horizontal direction adjusting assembly, the driving end of the horizontal direction adjusting assembly is fixedly connected with the roller type conveying assembly, and the roller type conveying assembly is fixedly connected with the clamping assembly; the fixed conveying assemblies are located at the two ends of the electronic weighing instrument, and the ends, close to the electronic weighing instrument, of the fixed conveying assemblies are rotationally connected with the adjustable conveying assembly. A liquid injection assembly for adding liquid is arranged on the rear side of the electronic weighing instrument; the liquid injection assembly is fixedly connected with a direction monitoring assembly used for detecting the position of a filling opening of the tank body and collecting dripping liquid of the liquid injection assembly. According to the invention, automatic filling for food processing enzyme preparation production is realized, and the automatic filling device is especially suitable for a tank body with a filling port staggered with the central axis of the tank body.

The invention relates to a method for processing enzymes comprising preparing an enzyme composition, exposing the enzyme composition to multiple transitions from a high pressure to a low pressure to provide a processed enzyme composition. The invention extends to a method for processing enzymes using an apparatus, a processed enzyme composition and methods of using the processed enzyme composition.

The utility model provides a Qilong capsule processing enzyme powder drying device, and mainly relates to the technical field of Qilong capsule processing enzyme powder drying equipment. A Qilong capsule processing enzyme powder drying device comprises a drying box, a motor is installed on one side of the drying box, a motor rotating shaft penetrates through the drying box, a connecting pipe is installed at one end of the motor rotating shaft through a coupler, and one end of the connecting pipe penetrates through the drying box and is rotationally connected with the drying box through a sealing bearing. The enzyme powder drying device has the beneficial effects that heated air is introduced into the connecting pipe through the air pump, is introduced into the drying plate through the vertical rod and is discharged from the air outlet in the drying plate, so that enzyme powder is heated and dried, and meanwhile, the connecting pipe and the drying plate can be driven to rotate under the driving of the motor, so that the enzyme powder is dried. The enzyme powder can be dried while being stirred, so that the flowability of the enzyme powder in the drying box is improved, the drying effect of the enzyme powder is improved, and convenience is brought to processing of the Qilong capsules.

The application discloses a method for improving expression of exogenous glycoprotein by yeast, comprising the following steps: without changing endogenous genes of a yeast host cell and without introducing exogenous sugar chain processing enzymes, an exogenous N-acetylglucosamine transferase I (GnT1) gene from different species is integrated into a genome of a Pichia pastoris host bacterium for expressing glycoprotein, and a Pichia pastoris engineering bacterium for expressing the exogenous GnT1 is constructed, so that the expression amount of the exogenous glycoprotein in the Pichia pastoris engineering bacterium is significantly improved, the proportion of high mannose type N-glycan is reduced, and the proportion of complex type N-glycan is increased. The method is suitable for biosynthesis of polysaccharide glycosylation protein, high isoelectric point protein and complex folding protein, and has good popularization and application prospect in the fields of biological pharmacy, functional protein, nutritional and healthy raw material and industrial biological manufacturing.

According to the invention, the action mechanism of key amino acids in the pullulanase PulA active framework is disclosed through the approach of structural analysis-site-directed mutagenesis-functional verification, the mutant enzyme with high catalytic efficiency is obtained, and a new direction is provided for the development of industrial starch processing enzyme resources. The purified mutant protein is successfully obtained, the enzyme activity of a wild type is improved to 296% through N98D, the activity of the wild type is improved to 157% through T175S, the activity of the wild type is improved to 170% through N379D, and the three mutants of N98D, T175S and N379D have important contributions to improvement of the enzyme activity and also have wide application prospects.

The present disclosure relates generally to engineered nucleic acid processing enzymes, based on DNA polymerases (e.g., engineered family B polymerases), and derivatives thereof having reverse transcriptase activity and substantially lacking strand displacement activity; kits comprising the engineered family B polymerases; and methods of generating and using the engineered family B polymerases.