Engineering intracellular sialylation pathways

a technology of intracellular sialylation and sialylation pathway, which is applied in the direction of transferases, enzymology, organic chemistry, etc., can solve the problems of limiting the wider application of this expression system, insect cell lines generating complex carbohydrates, and specific and limiting carbohydrate processing, so as to enhance the production of limiting components
US20050287637A1Inactive Publication Date: 2005-12-29BETENBAUGH MICHAEL +3
3 Cites 12 Cited by

Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
BETENBAUGH MICHAEL
Publication Date
2005-12-29
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

Methods for manipulating carbohydrate processing pathways in cells of interest are provided. Methods are directed at manipulating multiple pathways involved with the sialylation reaction by using recombinant DNA technology and substrate feeding approaches to enable the production of sialylated glycoproteins in cells of interest. These carbohydrate engineering efforts encompass the implementation of new carbohydrate bioassays, the examination of a selection of insect cell lines and the use of bioinformatics to identify gene sequences for critical processing enzymes. The compositions comprise cells of interest producing sialylated glycoproteins. The methods and compositions are useful for heterologous expression of glycoproteins.
Need to check novelty before this filing date? Find Prior Art

Description

[0001] This application claims benefit under 35 U.S.C. section 119(e) based on copendino U.S. Provisional Application Ser. No. 60 / 227,579, filed Aug. 25, 2000, which is herein incorporated by reference in its entirety, and also claims benefit under 35 U.S.C. section 120 to U.S. application Ser. No. 09 / 516,793, filed Mar. 1, 2000, which is herein incorporated by reference in its entirety and which claims benefit under 35 U.S.C. section 119(e) based on U.S. Provisional Applications Nos. 60 / 169,624, filed Dec. 8, 1999, and 60 / 122,582, filed Mar. 2, 1999, both of which are herein incorporated by reference in their entireties.

[0002] Part of the work performed during the development of this invention utilized U.S. Government funds in the form of grants from the National Science Foundation, Grant Numbers BES9814157, BES9814100, and the National Institutes of Health, Grant Number RO1-GM-49734. The U.S. Government has certain rights in this invention.FIELD OF THE INVENTION

[0003] The inventi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More