72results about How to "Improve targeting efficiency" patented technology

Methods of amplifying a target nucleic acid whereby the target nucleic acid in a sample is highly sensitively and specifically amplified; and compositions and kits to be used in these methods.

A composite magnetic nanoparticle drug delivery system provides targeted controlled release chemotherapies for cancerous tumors and inflammatory diseases. The magnetic nanoparticle includes a biocompatible and biodegradable polymer, a magnetic nanoparticle, the biological targeting agent human serum albumin, and a therapeutic pharmaceutical composition. The composite nanoparticles are prepared by oil-in-oil emulsion / solvent evaporation and high shear mixing. An externally applied magnetic field draws the magnetic nanoparticles to affected areas. The biological targeting agent draws the nanoparticles into the affected tissues. Polymer degradation provides controlled time release delivery of the pharmaceutical agent.

A magnetron sputtering apparatus is composed of a vacuum chamber (10), a target (15), a substrate (13), an anode (14) for supporting the substrate (13) that is disposed in the vacuum chamber, a cathodic body (16) for supporting the target (15) that is allocated so as to confront with the anode (14) and a magnetic field generating section (50) for generating a magnetic field on a surface of the target (15) that is allocated in neighborhood of one side of the cathodic body (16) opposite to the target (15). The target (15) is in a shape of square flat plate. The magnetic field generating section (50) is further composed of a yoke (51) in flat plate corresponding to the target (15), a first permanent magnet (52) in rectangular parallelepiped that is disposed in the middle of the yoke (51) and second and third permanent magnets (53, 54) in rectangular parallelepiped that are disposed in both end portions of the yoke (51) respectively. The magnetron sputtering apparatus is further composed of a driving unit (56) for swinging the magnetic field generating section (50) within a prescribed angle with centering a line as an axis of rotation, wherein the line passes through an approximate center (56) of the yoke (51) and is perpendicular to magnetic flux lines of the magnetic field and in parallel with the target (15).

Methods of amplifying a target nucleic acid whereby the target nucleic acid is amplified in the presence of a deoxyribonucleotide triphosphate, a DNA polymerase having strand displacement activity, at least one chimeric oligonucleotide primer, at least one upstream block oligonucleotide and a RNase H; wherein the chimeric oligonucleotide primer contains a ribonucleotide positioned at the 3′-terminus; wherein the upstream block oligonucleotide is capable of annealing to a region 3′ to a portion in the nucleic acid as the template to which the chimeric oligonucleotide primer anneals; and compositions and kits thereof.

A method for identifying a molecule that binds an irradiated tumor in a subject and molecules identified thereby. The method includes the steps of: (a) exposing a tumor to ionizing radiation; (b) administering to a subject a library of diverse molecules; and (c) isolating from the tumor one or more molecules of the library of diverse molecules, whereby a molecule that binds an irradiated tumor is identified. Also provided are therapeutic and diagnostic methods using targeting ligands that bind an irradiated tumor.

The invention relates to a hyaluronic-acid-based double-targeting nano-composite medicament and a preparation method thereof. Hydrophobic group ursodeoxycholic acid is included in a hyaluronic acid nano-polymer structure and can form an amphipathic polymer and automatically generate micelles in an aqueous solution, and polyethylene glycol can be introduced into the micelles to improve the dispersity and stability of a composite. An anti-tumor medicament can enter a nano-carrier through electrostatic adsorption or physical inclusion to generate a nano-medicament composite, wherein the nano-medicament composite is selectively concentrated in a tumor cell under an active targeting effect of hyaluronic acid and a surface CD44 receptor of a tumor cell, and promotes a tumor tissue to absorb the nano medicament-carrying composite by using a passive osmotic accumulation effect (EPR) at the same time. After an anti-tumor medicament is wrapped by a modified hyaluronic acid polymer, the anti-tumor medicament has the advantages of improving the bioavailability of the medicament, improving the targeting property, reducing the toxic and side effects, prolonging the half-life period of the medicament, being stably stored and the like, so that the tumor targeting therapy efficiency is improved in many ways.

A method for conversion of an animal into an appropriate recipient of tumor cells derived from a different species. Animals for such purpose can be immuno-incompetent animals that are doubly grafted with orthotopic tissues, in which one grafted tissue (i.e., breast) is from an organ of the same class as the tumor of origin (graft A), and the second grafted tissue (i.e., bone) is from a organ of the same class as a target organ for metastasis (graft B). These dual grafted animals can be used to model human diseases. In one implementation, human tumor cells are orthotopically seeded in graft A in order to analyze the occurrence of metastasis in graft B. Methods and compositions are described for creating a multiorgan human environment in mice, by grafting human stem cells (mesenchymal, embryonic or others) into mice, e.g., injured to enhance specific tissue engraftment. Such chimeric mice can be used to grow human tumors and to study the occurrence of metastasis.

The invention belongs to the field of pharmaceutical preparations, and relates to preparation of a novel target nano carrier and application of the novel target nano carrier in a camptothecin drug targeting delivery system. The system can be represented as PAMAM-HA / drug, wherein PAMAM is polyamide amine-amine arboraceous macromolecules of different generations, HA is hyaluronic acid of different molecular weights, and the drug is one or more of camptothecin anticancer drugs such as camptothecin, 10- hydroxycamptothecine, topotecan and the like. The carrier is used for wrapping the camptothecin drugs, and has the actions of prolonging the drug blood circulation time, improving the drug bioavailability, reducing the non-tumor part concentration of the drug, reducing the drug cytotoxicity, and simultaneously protecting the lactonic rings of the camptothecin drugs from damage, thereby improving the tumor treatment efficiency from multiple aspects. The nano carrier provided by the invention has important significance in promoting the safe delivery of the camptothecin drugs and the effective treatment of tumor.

The invention belongs to the medical technical field, and relates to a new function of high density lipoproteins (HDLs) of different structures as a targeted carrier for a liposoluble cardiovascular medicament. The recombinant HDL can be taken as a cholesterol receptor for receiving excess extra-hepatic cellular cholesterol and transporting the cholesterol to liver for metabolism, thus achieving the purpose of treating cardiovascular or arteriosclerotic disease. In the invention, the HDLs or lipid components thereof are recombined into the carrier for the liposoluble medicament in vitro so as to achieve dual functions of medicament delivery to an atherosclerosis focal area on a vascular wall and reverse cholesterol transport (RCT), which provides a new effective way for treating cardiovascular disease.

The information processing device comprises first and second master circuits and an arbiter for arbitrating access rights to a bus to which the master circuits are connected. The arbiter has storage units retaining information representing priorities of the access rights, and an arbitration control logical unit for arbitrating the access rights of the master circuits based on the information. When the priority of the first master circuit is higher than the priority of the second master circuit and there is no access request from the first master circuit but there is an access request from the second master circuit, the arbitration control logical unit permits access of the second master circuit, and the storage units lower the priority of the second master circuit without changing the priority of the first master circuit.

A magnetron sputtering apparatus in which a target is disposed to face a substrate includes a magnet array body including a magnet group arranged on a base body, and a rotating mechanism for rotating the magnet array body around an axis perpendicular to the substrate. In the magnet array body, N poles and S poles constituting the magnet group are arranged to be spaced from each other along a surface facing the target such that a plasma is generated based on a drift of electrons by a cusp magnetic field. Magnets located on the outermost periphery of the magnet group are arranged in a line to prevent the electrons from being released from constraint of the cusp magnetic field and jumping out of the cusp magnetic field. A distance between the target and the substrate during sputtering is equal to or less than 30 mm.

The invention discloses a dual-targeting ultrasonic contrast agent and a preparation method thereof. The contrast agent is an Integrin alpha v beta 3 / MCP-1 dual-targeting micro-vesicle, the dual-targeting micro-vesicle comprises a lipid shell and a gas inner core, and the external surface of the lipid shell is connected with an Integrin alpha v beta 3 monoclonal antibody and an MCP-1 monoclonal antibody. Through the combined application of different target spots, the targeting efficiency of the contrast agent can be effectively increased, and the degree of enrichment of the carrying genes in the target tissue can be greatly increased. In addition, the contrast agent disclosed by the invention is an ultrasonic contrast agent with double functions of development and treatment, and tumor images can be accurately observed in real time through an ultrasonic imaging system; and after gene transfection is realized through high-strength ultrasonic irradiation, the tumor gene treatment effect can be achieved.

The invention belongs to the field of medicinal preparations, and relates to a targeted nanometer delivery release system aiming at a multidrug resistant tumor and a preparation method of the targeted nanometer delivery release system. According to the system and the preparation method, the photosensitizer, namely, pyrophaeophorbide-a (PPA) is bonded to two ends of the copolymer, namely, hydroxylated-polylactic acid-polyglycolic acid (HO-PLA-PEG-PLA-OH) through a chemical bonding method, thus the product, namely, the photosensitizer-embedded polylactic acid-polyglycolic acid polymer (PPA-PLA-PEG-PLA-PPA) is obtained, then the chemotherapy medicine, namely, paclitaxel is encapsulated physically by adopting an emulsified solvent evaporation method, thus the binary-drug-loading nanometer delivery release system (PPA NP-PTX) is prepared, then F3 peptide with the specific targeting function and the cell penetrating function is modified on the surface of the system, and thus the photodynamic therapy and chemotherapy combined drug delivery nanometer delivery release system with the binary targeting property is prepared. The experiment proves that the delivery system has the strong lethal effect for drug resistant tumor cells, has the obvious in-vivo tumor targeting effect, and has the obvious treatment effect for the mice bearing the multidrug resistant tumor, and thus the combined drug delivery nanometer system has the clinical application prospect.

The invention relates to a hyperstable monodisperse fluorescent magnetic nano probe and preparation and application thereof. The fluorescent magnetic nano probe comprises a carrier and a light-sensitive agent, wherein the carrier is composed of carboxylated ferriferrous oxide magnetic nano particles, PEG and target molecules, and the light-sensitive agent is loaded on the carrier. The preparation method includes the specific steps that firstly, ascorbic acid is placed in an ferric salt solution, the pH value of the solution is adjusted to be 9-12, stirring and hydrothermal reaction are conducted, and an MNPs-COOH dispersion solution is obtained; secondly, the MNPs-COOH dispersion solution is centrifuged, then carboxyl groups of the MNPs-COOH dispersion solution are activated, PEG is added, mixing reaction is conducted, and an MNPs-PEG dispersion solution is obtained; thirdly, activated target molecules are added into and mixed with the MNPs-PEG dispersion solution for reaction, and an MNPs-PEG dispersion solution with the target molecules is obtained; fourthly, the MNPs-PEG dispersion solution with the target molecules and the light-sensitive agent solution are stirred and mixed and react, and a target product is obtained. The nano probe is used for dual-mode tomography in animal bodies. Compared with the prior art, the hyperstable monodisperse fluorescent magnetic nano probe has the advantages that targeting efficiency is high, residence time on tumor portions is long, and the preparation technology is simple.

The invention provides to improved methods for the modification of genes in plant cells, and plants and seeds derived therefrom. More specifically, the invention relates to the increased efficiency of targeted gene mutation by combining gene repair oligonucleotides with approaches that enhance the availability of components of the target cell gene repair mechanisms.

The invention relates to a nano-carrier entrapping anticancer drugs and gold nanoparticles lipids and a preparation method of the nano-carrier, and belongs to the technical field of medicines. The nano-carrier entraps the anticancer drugs and the gold nanoparticles jointly and then is modified by functional molecules and (or) high molecular polymers. Draw ratio of gold nanoparticles in the carrier is 1:1-8:1, average grain diameters are 1 nanometer to 100 nanometers, and the temperature can rise to 25-80 DEG C after solution of the nano-carrier is irradiated by near-infrared light. The average grain diameter of the lipid nano-carrier is 20 nanometers to 500 nanometers. The lipid nano-carrier has effects of tumor targeted chemical treatment and / or tumor thermal synergy therapy, toxic and side effects of the anticancer drugs can be reduced, and an anti-tumor effect is improved. A preparation technology is simple, and the stability of the lipid nano-carrier is high.

The invention relates to a targeted superparamagnetic monodisperse nano-flower probe, the preparation and the application thereof. A nano-flower probe is composed of three parts, namely a magnetic gold-bearing particle nano-flower, a hyperbranched macromolecular HPG and a target molecule, wherein one end of the hyperbranched macromolecular HPG is provided with a mercapto group and a branched terminal. The nano-flower probe is prepared through the hot solvent method. That is, firstly, a Fe3O4 carrier is prepared. Secondly, a golden seed is loaded on the carrier, and then the magnetic gold-bearing particle nano-flower is obtained. Finally, the magnetic gold-bearing particle nano-flower is connected sequentially with the HPG and the target molecule. The above nano-probe is applied to the oncotherapy for animal in-vivo double-targeted imaging under the alternating magnetic field. Compared with the prior art, the targeted superparamagnetic monodisperse nano-flower probe is simple to prepare, low in cost, high in targeting efficiency, and small in oncotherapy side effect. Compared with the prior art, the targeted superparamagnetic monodisperse nano-flower probe has the advantages of simple preparation, low cost, high targeting efficiency, small oncotherapy side effect and the like.

The invention relates to a preparation method and applications of a double-stage brain-targeting polymer micelle drug delivery system. According to the preparation method, the double-stage targeting micelle is formed via the subject-object interaction effect between hydrophilic molecules (Ang-2-polyethylene glycol-beta-cyclodextrin and cRGD-polyethylene glycol-beta-cyclodextrin) and hydrophobic molecule (adamantine-polycaprolactone-fluorescein isothiocyanate). The core of the double-stage targeting micelle can be used for coating hydrophobic anti-tumor drugs; entering into glioma cells throughblood cerebral barrier is realized by surface targeting peptide (Ang-2 and cRGD) induced endocytic pathway; chemotherapeutic curative effect is improved; toxic or side effect is reduced; the micelleis high in stability; the hydrophilic and hydrophobic chain length and the ratio are controllable; targeting efficiency is high; the preparation method is simple; operation is convenient; and subsequent development and industrialization are convenient.

Provided is an apparatus for capturing a target component from a gas including a rotating packed bed and a packed bed. The rotating packed bed has a first absorbent inlet, a first absorbent outlet, a first gas inlet and a first gas outlet. The packed bed has a second absorbent inlet, a second absorbent outlet, a second gas inlet and a second gas outlet. The first absorbent outlet is in connection with the second absorbent inlet to form an absorbent flow path that sequentially passes through the rotating packed bed and the packed bed. The second gas outlet is in connection with the first gas inlet to form a gas flow path that sequentially passes through the packed bed and the rotating packed bed.

An object of the present invention is to provide a gasket capable of improving cooling efficiency with a simpler configuration while keeping performance of the gasket and a manufacturing method thereof. A gasket includes a first annular portion which is formed by a metal wire woven fabric obtained by weaving a first metal wire and includes a seal target hole and a main body portion which is in contact with an outer peripheral edge of the first annular portion, in which the first metal wire forming the first annular portion and the first metal wire forming the main body portion are entangled with each other and a second metal wire is woven together with the first metal wire forming the first annular portion to form the first annular portion as a high thermal conduction region.

The invention provides a military-police integrated laser automatic target-scoring device with high precision and relates to training equipment. According to the automatic target-scoring device, a point of impact can be rapidly determined, and automatic target scoring is realized. The technical scheme is that the automatic target-scoring device comprises a square target frame, laser transmitting tubes, laser receiving tubes, a laser transmitter, a microcontroller and an upper computer, wherein bullet-proof glass is inlaid in the target frame, and edges of the bullet-proof glass are connected with the inner side surfaces of the target frame; the laser transmitting tubes are arranged on the four inner side surfaces of the target frame; multiple laser receiving tubes are arranged on two sides of each laser transmitting tube; the laser transmitting tubes are connected with the laser transmitter; the laser receiving tubes are connected with the microcontroller through data lines; the microcontroller is connected with the upper computer.

The invention discloses nanoparticles prepared by applying a microfluidic technology, a preparation method and device and application of the nanoparticles. The particle size of the nanoparticles is 175nm-225nm, the particle size dispersity is 15%-30%, and the electric potential is -25MV to -5MV. The method comprises the following steps: completely dissolving shellac in a polar solvent, and then, dissolving a medicine CAPE in the polar solvent to prepare an internal phase solution; dissolving Arabic gum in deionized water to prepare an external water phase solution; wrapping the internal phase solution in the external water phase solution, and preparing a nanoparticle solution by adopting a one-step method device; and carrying out rotary evaporation on the nanoparticle solution to remove the solvent from the solution, and carrying out drying, thereby obtaining the product. The nanoparticles prepared by the invention are loaded with the medicine CAPE and have good dispersibility and stability, the structure and activity of the encapsulated CAPE are protected in a stomach, and the encapsulated CAPE is specifically released in an intestinal tract region.

The present invention concerns a method for modulating double-strand break-induced homologous recombination through the identification of effectors that modulate said double-strand break-induced homologous recombination by uses of interfering agents; these agents are capable of modulating double-strand break-induced homologous recombination through their respective actions on said effectors. The present invention also concerns the uses of these effectors and interfering agents and derivatives, respectively, by introducing them in an eukaryotic cell in order to modulate and more particularly to increase double-strand break-induced homologous recombination and gene targeting efficiency. The present invention also relates to specific derivatives of identified effectors and interfering agents, vectors encoding them, compositions and kits comprising such derivatives in order to modulate and more particularly to increase double-strand break-induced homologous recombination and gene targeting efficiency.

The invention discloses a method for preparing a dual-hepatic-targeting long-circulation gypenoside liposome by modifying beta-sitosterol by virtue of galacturonic acid, glycyrrhetinic acid and polyethylene glycol. Compared with a normal gynostemma liposome, the targeting efficiency to parenchymal hepatic cells and long-circulation effect of the gypenoside liposome are greatly improved. The novel long-circulation liposome is good in hepatic targeting property, and can also obviously prolong the retention time of the gypenoside in a body, so that the gypenoside can actively target to hepatic tumor cells, and an effect of treating tumors is enhanced.

The molecular mechanisms of peroxisome biogenesis have begun to emerge: in contrast, relatively little is known about how the organelle functions as cells age. The present inventors characterized age-related changes in peroxisomes of human cells and showed that aging compromises peroxisomal targeting signal 1 (PTS1) protein import, with the critical antioxidant enzyme, catalase, especially affected. The number and appearance of peroxisomes are altered in these cells, and the organelles accumulate the PTS1-import receptor. Pex5p, on their membranes. Concomitantly, cells produce increasing amounts of the toxic metabolite, H2O2, and this increased load of reactive oxygen species (ROS) may further reduce peroxisomal protein import and exacerbate the effects of aging. Disclosed are novel compositions and methods for restoring catalase in peroxisomes by use of targeted catalase modified at its C-terminus and / or N-terminus, optionally in combination with polypeptides which promote cellular uptake of proteins, to prevent or overcome the changes that follows aging or that are associated with a number of diseases or disorders.

The invention relates to a hyperstable monodisperse fluorescent magnetic diagnosis and treatment nanoprobe and a preparation method and application thereof. The nanoprobe comprises magnetic nano particles coating polyethylene glycol PEG and a CaCO3 coating layer doped with a photosensitizer, wherein a mass ratio of the magnetic nano particles to the CaCO3 coating layer is 10 to (1 to 3), and the load of the photosensitizer is 8.9-10.3 wt%; ascorbic acid is placed in an iron salt solution, a solution pH is adjusted to be 9 to 12, and through hydrothermal reaction, magnetic nano particle dispersion liquid is obtained; CaCl2 and ICG are added into the magnetic nano particle dispersion liquid, reaction is carried out while stirring, and then Na2CO3 and ICG are added for reaction for 24 hours to obtain a target product. The nanoprobe is used for bimodal imaging in an animal body, and has the advantages that blood circulation time is long, tumor location retention time is long, the medicinetargeted delivery efficiency is high, a preparation technology is simple, and the like.

The present invention concerns a method for modulating double-strand break-induced homologous recombination through the identification of effectors that modulate said double-strand break-induced homologous recombination by uses of interfering agents; these agents are capable of modulating double-strand break-induced homologous recombination through their respective actions on said effectors. The present invention also concerns the uses of these effectors and interfering agents and derivatives, respectively, by introducing them in an eukaryotic cell in order to modulate and more particularly to increase double-strand break-induced homologous recombination and gene targeting efficiency. The present invention also relates to specific derivatives of identified effectors and interfering agents, vectors encoding them, compositions and kits comprising such derivatives in order to modulate and more particularly to increase double-strand break-induced homologous recombination and gene targeting efficiency.

A sputter deposition apparatus can sputter a wider surface area of a sputtering surface of a target in comparison to an area that could be sputtered by a conventional apparatus. An adhesion-preventing member surrounding the outer periphery of a sputtering surface of a target made of a metal material is formed by insulating ceramic. The target is sputtered while moving a magnet device between a position where the entire outer periphery of an outer peripheral magnet is on the inside of the outer periphery of the sputtering surface and another position where a part of the outer periphery of the outer peripheral magnet protrudes out to the outside of the outer periphery of the sputtering surface.

The invention relates to a preparation method of a double-target modified liposome. The preparation method includes the steps that (1) target molecules are respectively connected to phospholipid molecules, target molecules include glycyrrhetinic acid and peanut agglutinin, and DSPE-PEG<2000>-GA and DSPE-PEG<2000>-PNA are obtained after connection; the DSPE-PEG<2000>-GA and the DSPE-PEG<2000>-PNA obtained in the step (1) are taken and dissolved in chloroform with soybean phospholipid and cholesterol, and a blank liposome is prepared by adopting a thin film dispersion method; and (3) the obtained blank liposome in the step (2) is taken, and a drug carrying liposome is prepared by adopting an ammonium sulfate gradient method. According to the preparation method, the target molecules are firstly connected to phospholipid and added with the soybean phospholipid and the cholesterol when the liposome is prepared, the connection efficiency is high, operation is convenient, and transformation is facilitated; and the two-target modified liposome obtained by the preparation method improves the targeting efficiency, and has obvious inhibitory effect on liver cancer cells.