10results about How to "Improve targeting efficiency" patented technology

The present invention proposes a durable three-axis drive mechanism for a minimally invasive cell puncture instrument, which belongs to the field of precision transmission technology. The drive mechanism includes an X-degree-of-freedom motion table, a Y-degree-of-freedom motion table, and a Z-degree-of-freedom motion table connected in series; the X-degree-of-freedom motion table, the Y-degree-of-freedom motion table, and the Z-degree-of-freedom motion table all include a base, on which a stator assembly, a pre-tightening magnet, a magnetic friction foot, and a link magnet and a piezoelectric body are installed; the stator assembly consists of a slide rail and a slider, the slide rail is fixed on the base, and the slider forms a sliding fit with the slide rail, and the sliding directions of the sliders of the X-degree-of-freedom motion table, the Y-degree-of-freedom motion table, and the Z-degree-of-freedom motion table are their degrees of freedom directions, respectively. The present invention realizes minimally invasive cell puncture based on the inertial drive principle, and has the advantages of simple structure, strong wear resistance, wear self-adaptation, modular replacement, and no need for pre-tightening and loosening prevention, and has the potential to achieve durable drive.

The invention discloses a targeted nano photosensitizer carrier and a preparation method and application thereof, and relates to the technical field of photosensitizer carriers, the preparation method of the targeted nano photosensitizer carrier comprises the following steps: activating 4-carboxyphenylboronic acid, dropwise adding the activated 4-carboxyphenylboronic acid into a methanol solution of polyethyleneimine, and reacting to obtain phenylboronic acid modified polyethyleneimine; dissolving the polyethyleneimine into a phosphate buffer solution, dropwise adding a methanol solution of 1, 2, 4-trihydroxy anthraquinone, and reacting in a dark place to obtain anthraquinone-boric acid ester modified polyethyleneimine; and activating and reacting with a folic acid solution to obtain the folic acid. The prepared targeted nano photosensitizer carrier can stably load a photosensitizer containing an aromatic ring structure, realizes light-controlled release and pH response, can specifically recognize tumor cells with high folate receptor expression, and has a wide application prospect in the field of photodynamic therapy.

This invention relates to novel, highly efficient subcellular targeted drug delivery conjugates, their preparation methods, compositions, and uses. The conjugates include a carrier module, a controlled-release module, a connecting arm, a subcellular targeting module, and a pharmacodynamic module. The conjugates of this invention can be used in applications such as highly efficient photodynamic therapy, chemotherapy, and immunotherapy targeting organelles, or in combination thereof.

The invention relates to the technical field of dexamethasone pharmaceutical preparations, and discloses a dexamethasone liposome targeting synovial fibroblasts and a preparation method of the dexamethasone liposome, the liposome comprises dexamethasone and a lipid bilayer used for wrapping the dexamethasone, and the lipid bilayer comprises DSPE-PEG2000-GPA-KLVFF. The preparation method comprises the following steps: step S1, preparation of DSPE-PEG2000-GPA-KLVFF (Distearoyl Pyrrolidone-Polyethylene Glycol 2000); s2, the soybean lecithin, the cholesterol, the DSPE-PEG2000-GPA-KLVFF and the dexamethasone are weighed according to the proportion, and the soybean lecithin, the cholesterol, the DSPE-PEG2000-GPA- And evaporating the solvent, and preparing the dexamethasone liposome by adopting a film dispersion method. The dexamethasone liposome prepared by the invention has definite in-vivo synovial fibroblasts and RA inflammation joint targeting characteristics, is good in dispersity and stability and high in FLS targeting efficiency, reduces MHC-II expression and inhibits RA inflammation development.

The present invention relates to: a toll-like receptor 7 or 8 agonist-drug complex material in which a functional drug and a toll-like receptor 7 or 8 agonist are bonded by a chemical bond containing a cleavable site; and using the properties of the temporarily inactivated state, the side effects of the toll-like receptor 7 or 8 agonist can be reduced. In particular, the complex material is designed such that the immunostimulatory function of the toll-like receptor 7 or 8 agonist is inhibited due to the chemical bond, while the immunostimulatory function is recovered at an injection site or in a tumor microenvironment or target immune cells, thus exhibiting kinetic properties in which immunostimulatory efficacy can be controlled over time, and minimizing the induction of nonspecific immune responses. In addition, since various functions can be combined with a drug, the inactivated toll-like receptor 7 or 8 agonist-functional drug complex can be widely used in pharmaceutical compositions for various applications, which are capable of controlling cancer cell death, the activation of therapeutic immune cells, and immunosuppressive cells and environments, at an injection site or in a tumor microenvironment.

The application discloses a ROS response type selenium-cerium composite nanometer enzyme and a preparation and application thereof, and comprises the following steps: S1, preparation of an organic silicon source double-[3-(triethoxy)silyl propyl] diselenide silane; S2, preparation of a mesoporous organic silicon nanometer material bridged by a diselenide bond; S3, preparation of a hyaluronic acid coated cerium dioxide nanometer enzyme; and S4, preparation of the ROS response type selenium-cerium composite nanometer enzyme. Through the three core innovations of embedding a diselenide bond skeleton, preloading HA-CeO2 in a channel and multi-enzyme simulation cooperation, the application systematically solves the key bottlenecks of stability, functionality, targeting and antioxidant efficiency of the existing nanometer platform, and provides a high-efficiency, safe and intelligent drug-free nanometer treatment new strategy for acute kidney injury.

The invention discloses small interfering RNA (Ribonucleic Acid) nanoparticles as well as a preparation method and application thereof. The small interfering RNA nanoparticle comprises a polyethyleneimine vector, a small interfering RNA and an sp13c aptamer, wherein the polyethyleneimine vector is a polyethyleneimine vector; the nucleotide sequence of the polyethyleneimine vector is as shown in SEQ ID NO.3, the nucleotide sequence of the small interfering RNA is as shown in SEQ ID NO.11, and the compounding ratio of the polyethyleneimine vector to the small interfering RNA to the sp13c aptamer is (0.2-2): (0.5-5): (0.2-2). The drug delivery carrier of the small interfering RNA is prepared by adopting a rolling circle transcription method, meanwhile, the sp13c aptamer is used as a bladder cancer targeting molecule of the nano delivery system, the drug loading capacity of the small interfering RNA is high, and compared with a control group of an unmodified sp13c aptamer, the bladder cancer cell targeting efficiency of the small interfering RNA is effectively improved.

The invention belongs to the technical field of novel small-molecule drugs, and particularly relates to a zwitterionic fluorescent probe containing a dimer targeting ligand and a preparation method and application thereof. The chemical structural formula of the zwitterionic fluorescent probe containing the dimer targeting ligand is as shown in formula (1): # imgabs0 #, the fluorescent probe can be used for near-infrared fluorescence imaging of tumor targeting, and the structure of the fluorescent probe contains two same tumor targeting ligands, so that the zwitterionic fluorescent probe has a better targeting function compared with a single targeting ligand. The introduction of the alkaline amino acid in the fluorescent probe enables the charge on the surface of the probe to return to zero, and the zwitterionic design enables the probe to have a lower tissue background signal. The probe disclosed by the invention is completely symmetrical in structure, simpler in synthesis method, good in water solubility, low in non-specific fluorescence signal and suitable for industrial production and clinical application.

The invention provides a magnetic self-assembly OPN protein detection nanoprobe as well as a construction method and application thereof, and belongs to the technical field of biological analysis. An alpha-Fe2O3 / Fe3O4 (at) Au-Report / MCH electrode modifier and a CRISPR / Cas13a system are mixed and then act to obtain a magnetic alpha-Fe2O3 / Fe3O4 (at) Au-MCH nano-composite, and the nano-composite is assembled to the surface of MGCE by utilizing the magnetism of the nano-composite to form the magnetic self-assembly OPN protein detection nano-probe. The magnetic self-assembly OPN protein detection nanoprobe can be used as a working electrode of an electrochemical RNA aptamer sensor for detecting the content of OPN protein; the electrochemical RNA aptamer sensor has a relatively low detection limit (0.33 pg / mL), a relatively wide linear range (1 pg / mL to 1 ng / mL) and relatively high sensitivity, and has good practicability.

The application relates to a composition for liver cancer imaging and treatment, relates to the field of tumor detection, realizes efficient and accurate cancer tissue identification by identifying specific markers on the surface of tumor cells or tissues, and further finds a highly potential liver cancer marker.