70results about How to "Improve targeting efficiency" patented technology

A composite magnetic nanoparticle drug delivery system provides targeted controlled release chemotherapies for cancerous tumors and inflammatory diseases. The magnetic nanoparticle includes a biocompatible and biodegradable polymer, a magnetic nanoparticle, the biological targeting agent human serum albumin, and a therapeutic pharmaceutical composition. The composite nanoparticles are prepared by oil-in-oil emulsion/solvent evaporation and high shear mixing. An externally applied magnetic field draws the magnetic nanoparticles to affected areas. The biological targeting agent draws the nanoparticles into the affected tissues. Polymer degradation provides controlled time release delivery of the pharmaceutical agent.

A magnetron sputtering apparatus is composed of a vacuum chamber (10), a target (15), a substrate (13), an anode (14) for supporting the substrate (13) that is disposed in the vacuum chamber, a cathodic body (16) for supporting the target (15) that is allocated so as to confront with the anode (14) and a magnetic field generating section (50) for generating a magnetic field on a surface of the target (15) that is allocated in neighborhood of one side of the cathodic body (16) opposite to the target (15). The target (15) is in a shape of square flat plate. The magnetic field generating section (50) is further composed of a yoke (51) in flat plate corresponding to the target (15), a first permanent magnet (52) in rectangular parallelepiped that is disposed in the middle of the yoke (51) and second and third permanent magnets (53, 54) in rectangular parallelepiped that are disposed in both end portions of the yoke (51) respectively. The magnetron sputtering apparatus is further composed of a driving unit (56) for swinging the magnetic field generating section (50) within a prescribed angle with centering a line as an axis of rotation, wherein the line passes through an approximate center (56) of the yoke (51) and is perpendicular to magnetic flux lines of the magnetic field and in parallel with the target (15).

The invention relates to a hyaluronic-acid-based double-targeting nano-composite medicament and a preparation method thereof. Hydrophobic group ursodeoxycholic acid is included in a hyaluronic acid nano-polymer structure and can form an amphipathic polymer and automatically generate micelles in an aqueous solution, and polyethylene glycol can be introduced into the micelles to improve the dispersity and stability of a composite. An anti-tumor medicament can enter a nano-carrier through electrostatic adsorption or physical inclusion to generate a nano-medicament composite, wherein the nano-medicament composite is selectively concentrated in a tumor cell under an active targeting effect of hyaluronic acid and a surface CD44 receptor of a tumor cell, and promotes a tumor tissue to absorb the nano medicament-carrying composite by using a passive osmotic accumulation effect (EPR) at the same time. After an anti-tumor medicament is wrapped by a modified hyaluronic acid polymer, the anti-tumor medicament has the advantages of improving the bioavailability of the medicament, improving the targeting property, reducing the toxic and side effects, prolonging the half-life period of the medicament, being stably stored and the like, so that the tumor targeting therapy efficiency is improved in many ways.

A method for conversion of an animal into an appropriate recipient of tumor cells derived from a different species. Animals for such purpose can be immuno-incompetent animals that are doubly grafted with orthotopic tissues, in which one grafted tissue (i.e., breast) is from an organ of the same class as the tumor of origin (graft A), and the second grafted tissue (i.e., bone) is from a organ of the same class as a target organ for metastasis (graft B). These dual grafted animals can be used to model human diseases. In one implementation, human tumor cells are orthotopically seeded in graft A in order to analyze the occurrence of metastasis in graft B. Methods and compositions are described for creating a multiorgan human environment in mice, by grafting human stem cells (mesenchymal, embryonic or others) into mice, e.g., injured to enhance specific tissue engraftment. Such chimeric mice can be used to grow human tumors and to study the occurrence of metastasis.

The invention belongs to the field of pharmaceutical preparations, and relates to preparation of a novel target nano carrier and application of the novel target nano carrier in a camptothecin drug targeting delivery system. The system can be represented as PAMAM-HA/drug, wherein PAMAM is polyamide amine-amine arboraceous macromolecules of different generations, HA is hyaluronic acid of different molecular weights, and the drug is one or more of camptothecin anticancer drugs such as camptothecin, 10- hydroxycamptothecine, topotecan and the like. The carrier is used for wrapping the camptothecin drugs, and has the actions of prolonging the drug blood circulation time, improving the drug bioavailability, reducing the non-tumor part concentration of the drug, reducing the drug cytotoxicity, and simultaneously protecting the lactonic rings of the camptothecin drugs from damage, thereby improving the tumor treatment efficiency from multiple aspects. The nano carrier provided by the invention has important significance in promoting the safe delivery of the camptothecin drugs and the effective treatment of tumor.

The invention belongs to the medical technical field, and relates to a new function of high density lipoproteins (HDLs) of different structures as a targeted carrier for a liposoluble cardiovascular medicament. The recombinant HDL can be taken as a cholesterol receptor for receiving excess extra-hepatic cellular cholesterol and transporting the cholesterol to liver for metabolism, thus achieving the purpose of treating cardiovascular or arteriosclerotic disease. In the invention, the HDLs or lipid components thereof are recombined into the carrier for the liposoluble medicament in vitro so as to achieve dual functions of medicament delivery to an atherosclerosis focal area on a vascular wall and reverse cholesterol transport (RCT), which provides a new effective way for treating cardiovascular disease.

A magnetron sputtering apparatus in which a target is disposed to face a substrate includes a magnet array body including a magnet group arranged on a base body, and a rotating mechanism for rotating the magnet array body around an axis perpendicular to the substrate. In the magnet array body, N poles and S poles constituting the magnet group are arranged to be spaced from each other along a surface facing the target such that a plasma is generated based on a drift of electrons by a cusp magnetic field. Magnets located on the outermost periphery of the magnet group are arranged in a line to prevent the electrons from being released from constraint of the cusp magnetic field and jumping out of the cusp magnetic field. A distance between the target and the substrate during sputtering is equal to or less than 30 mm.

The invention discloses a dual-targeting ultrasonic contrast agent and a preparation method thereof. The contrast agent is an Integrin alpha v beta 3/MCP-1 dual-targeting micro-vesicle, the dual-targeting micro-vesicle comprises a lipid shell and a gas inner core, and the external surface of the lipid shell is connected with an Integrin alpha v beta 3 monoclonal antibody and an MCP-1 monoclonal antibody. Through the combined application of different target spots, the targeting efficiency of the contrast agent can be effectively increased, and the degree of enrichment of the carrying genes in the target tissue can be greatly increased. In addition, the contrast agent disclosed by the invention is an ultrasonic contrast agent with double functions of development and treatment, and tumor images can be accurately observed in real time through an ultrasonic imaging system; and after gene transfection is realized through high-strength ultrasonic irradiation, the tumor gene treatment effect can be achieved.

The invention belongs to the field of medicinal preparations, and relates to a targeted nanometer delivery release system aiming at a multidrug resistant tumor and a preparation method of the targeted nanometer delivery release system. According to the system and the preparation method, the photosensitizer, namely, pyrophaeophorbide-a (PPA) is bonded to two ends of the copolymer, namely, hydroxylated-polylactic acid-polyglycolic acid (HO-PLA-PEG-PLA-OH) through a chemical bonding method, thus the product, namely, the photosensitizer-embedded polylactic acid-polyglycolic acid polymer (PPA-PLA-PEG-PLA-PPA) is obtained, then the chemotherapy medicine, namely, paclitaxel is encapsulated physically by adopting an emulsified solvent evaporation method, thus the binary-drug-loading nanometer delivery release system (PPA NP-PTX) is prepared, then F3 peptide with the specific targeting function and the cell penetrating function is modified on the surface of the system, and thus the photodynamic therapy and chemotherapy combined drug delivery nanometer delivery release system with the binary targeting property is prepared. The experiment proves that the delivery system has the strong lethal effect for drug resistant tumor cells, has the obvious in-vivo tumor targeting effect, and has the obvious treatment effect for the mice bearing the multidrug resistant tumor, and thus the combined drug delivery nanometer system has the clinical application prospect.

Provided is an apparatus for capturing a target component from a gas including a rotating packed bed and a packed bed. The rotating packed bed has a first absorbent inlet, a first absorbent outlet, a first gas inlet and a first gas outlet. The packed bed has a second absorbent inlet, a second absorbent outlet, a second gas inlet and a second gas outlet. The first absorbent outlet is in connection with the second absorbent inlet to form an absorbent flow path that sequentially passes through the rotating packed bed and the packed bed. The second gas outlet is in connection with the first gas inlet to form a gas flow path that sequentially passes through the packed bed and the rotating packed bed.

The invention provides a military-police integrated laser automatic target-scoring device with high precision and relates to training equipment. According to the automatic target-scoring device, a point of impact can be rapidly determined, and automatic target scoring is realized. The technical scheme is that the automatic target-scoring device comprises a square target frame, laser transmitting tubes, laser receiving tubes, a laser transmitter, a microcontroller and an upper computer, wherein bullet-proof glass is inlaid in the target frame, and edges of the bullet-proof glass are connected with the inner side surfaces of the target frame; the laser transmitting tubes are arranged on the four inner side surfaces of the target frame; multiple laser receiving tubes are arranged on two sides of each laser transmitting tube; the laser transmitting tubes are connected with the laser transmitter; the laser receiving tubes are connected with the microcontroller through data lines; the microcontroller is connected with the upper computer.

The invention discloses nanoparticles prepared by applying a microfluidic technology, a preparation method and device and application of the nanoparticles. The particle size of the nanoparticles is 175nm-225nm, the particle size dispersity is 15%-30%, and the electric potential is -25MV to -5MV. The method comprises the following steps: completely dissolving shellac in a polar solvent, and then, dissolving a medicine CAPE in the polar solvent to prepare an internal phase solution; dissolving Arabic gum in deionized water to prepare an external water phase solution; wrapping the internal phase solution in the external water phase solution, and preparing a nanoparticle solution by adopting a one-step method device; and carrying out rotary evaporation on the nanoparticle solution to remove the solvent from the solution, and carrying out drying, thereby obtaining the product. The nanoparticles prepared by the invention are loaded with the medicine CAPE and have good dispersibility and stability, the structure and activity of the encapsulated CAPE are protected in a stomach, and the encapsulated CAPE is specifically released in an intestinal tract region.

The present invention concerns a method for modulating double-strand break-induced homologous recombination through the identification of effectors that modulate said double-strand break-induced homologous recombination by uses of interfering agents; these agents are capable of modulating double-strand break-induced homologous recombination through their respective actions on said effectors. The present invention also concerns the uses of these effectors and interfering agents and derivatives, respectively, by introducing them in an eukaryotic cell in order to modulate and more particularly to increase double-strand break-induced homologous recombination and gene targeting efficiency. The present invention also relates to specific derivatives of identified effectors and interfering agents, vectors encoding them, compositions and kits comprising such derivatives in order to modulate and more particularly to increase double-strand break-induced homologous recombination and gene targeting efficiency.

The invention discloses a method for preparing a dual-hepatic-targeting long-circulation gypenoside liposome by modifying beta-sitosterol by virtue of galacturonic acid, glycyrrhetinic acid and polyethylene glycol. Compared with a normal gynostemma liposome, the targeting efficiency to parenchymal hepatic cells and long-circulation effect of the gypenoside liposome are greatly improved. The novel long-circulation liposome is good in hepatic targeting property, and can also obviously prolong the retention time of the gypenoside in a body, so that the gypenoside can actively target to hepatic tumor cells, and an effect of treating tumors is enhanced.

The molecular mechanisms of peroxisome biogenesis have begun to emerge: in contrast, relatively little is known about how the organelle functions as cells age. The present inventors characterized age-related changes in peroxisomes of human cells and showed that aging compromises peroxisomal targeting signal 1 (PTS1) protein import, with the critical antioxidant enzyme, catalase, especially affected. The number and appearance of peroxisomes are altered in these cells, and the organelles accumulate the PTS1-import receptor. Pex5p, on their membranes. Concomitantly, cells produce increasing amounts of the toxic metabolite, H₂O₂, and this increased load of reactive oxygen species (ROS) may further reduce peroxisomal protein import and exacerbate the effects of aging. Disclosed are novel compositions and methods for restoring catalase in peroxisomes by use of targeted catalase modified at its C-terminus and/or N-terminus, optionally in combination with polypeptides which promote cellular uptake of proteins, to prevent or overcome the changes that follows aging or that are associated with a number of diseases or disorders.

The invention relates to a hyperstable monodisperse fluorescent magnetic diagnosis and treatment nanoprobe and a preparation method and application thereof. The nanoprobe comprises magnetic nano particles coating polyethylene glycol PEG and a CaCO3 coating layer doped with a photosensitizer, wherein a mass ratio of the magnetic nano particles to the CaCO3 coating layer is 10 to (1 to 3), and the load of the photosensitizer is 8.9-10.3 wt%; ascorbic acid is placed in an iron salt solution, a solution pH is adjusted to be 9 to 12, and through hydrothermal reaction, magnetic nano particle dispersion liquid is obtained; CaCl2 and ICG are added into the magnetic nano particle dispersion liquid, reaction is carried out while stirring, and then Na2CO3 and ICG are added for reaction for 24 hours to obtain a target product. The nanoprobe is used for bimodal imaging in an animal body, and has the advantages that blood circulation time is long, tumor location retention time is long, the medicinetargeted delivery efficiency is high, a preparation technology is simple, and the like.

A sputter deposition apparatus can sputter a wider surface area of a sputtering surface of a target in comparison to an area that could be sputtered by a conventional apparatus. An adhesion-preventing member surrounding the outer periphery of a sputtering surface of a target made of a metal material is formed by insulating ceramic. The target is sputtered while moving a magnet device between a position where the entire outer periphery of an outer peripheral magnet is on the inside of the outer periphery of the sputtering surface and another position where a part of the outer periphery of the outer peripheral magnet protrudes out to the outside of the outer periphery of the sputtering surface.

The invention relates to a preparation method of a double-target modified liposome. The preparation method includes the steps that (1) target molecules are respectively connected to phospholipid molecules, target molecules include glycyrrhetinic acid and peanut agglutinin, and DSPE-PEG<2000>-GA and DSPE-PEG<2000>-PNA are obtained after connection; the DSPE-PEG<2000>-GA and the DSPE-PEG<2000>-PNA obtained in the step (1) are taken and dissolved in chloroform with soybean phospholipid and cholesterol, and a blank liposome is prepared by adopting a thin film dispersion method; and (3) the obtained blank liposome in the step (2) is taken, and a drug carrying liposome is prepared by adopting an ammonium sulfate gradient method. According to the preparation method, the target molecules are firstly connected to phospholipid and added with the soybean phospholipid and the cholesterol when the liposome is prepared, the connection efficiency is high, operation is convenient, and transformation is facilitated; and the two-target modified liposome obtained by the preparation method improves the targeting efficiency, and has obvious inhibitory effect on liver cancer cells.

The invention relates to an ATP sensitive liposome with a tumor near infrared fluorescence development effect and a treatment effect. ATP sensitive nucleotide double strands loaded with adriamycin are encapsulated into a liposome dimolecular layer membrane coupled with tumor target polypeptide, so that a nanovesicle is formed, the ATP sensitive nucleotide double strands are formed by self-assembling of ATP sensitive nucleotide single strand coupled with fluorescent dye and ATP sensitive nucleotide complementary single strands coupled with a fluorescence quenching agent according to a base complementarity pairing principle, and the liposome dimolecular layer membrane coupled with the tumor target polypeptide consists of polyethylene glycol phosphatidyl ethanolamine coupled by the tumor target polypeptide, phosphatide and cholesterol. The invention further provides a preparation method of the liposome. The liposome concurrently has an active tumor targeting property, an ATP sensitive fluorescence emitting function, a medicine release switching function and an in vivo long-circulating function, the purpose of combining function like tumor near infrared fluorescence development, tumor diagnosis and tumor treatment in the same liposome is achieved, and besides, the preparation technology is simple and easy to operate.

A sample preparation apparatus for DNA analysis comprises a holder for separating specific primers on the basis of size, color, weight, dimension, or degree of magnetization, the specific primers having base sequences complementary to a plurality of DNA fragments to be amplified via PCR, and the specific primers being capable of binding respectively to the DNA fragments; and a reaction-solution-holding plate having a concavity which accommodates one edge of the holder and a PCR solution containing a common primer capable of hybridizing with the base sequence of an oligonucleotide introduced into the 5′-end of each of the DNA fragments, and the DNA fragments. The PCR amplification of the DNA fragments is carried out by using the specific primers (immobilized on the surfaces of a plurality of mutually separable supports with respect to each DNA fragments) and the common primer (a mobile primer common to all DNA fragments) to produce PCR amplification products inside the corresponding portions of the holder. The DNA fragments are derived from a plurality of DNAs to be amplified by PCR under the same conditions at the same time to avoid undesired mutual interference among the primers, and the PCR products can be separated and recovered for each of the DNA fragments. The sample preparation method for DNA analysis comprises the relevant amplifying, separating and recovering steps as described above.