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Hyperstable monodisperse fluorescent magnetic diagnosis and treatment nanoprobe and preparation method and application thereof

A nano-probe and magnetic nano-technology, which is applied in the field of ultra-stable monodisperse fluorescent magnetic diagnosis and treatment nano-probes and its preparation, can solve the problems of retention time and aggregation amount of tumor sites that are difficult to achieve as desired, and achieve magnetic properties Excellent, high targeting efficiency, simple preparation steps

Inactive Publication Date: 2019-03-12
苏州纳葛诺斯生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, some researchers have combined the advantages of magnetic resonance imaging and fluorescence imaging of photosensitizers to design fluorescent magnetic nanoprobes, but their residence time and accumulation in normal tissues (such as: liver, spleen, etc.) It is still difficult to achieve satisfactory results

Method used

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  • Hyperstable monodisperse fluorescent magnetic diagnosis and treatment nanoprobe and preparation method and application thereof
  • Hyperstable monodisperse fluorescent magnetic diagnosis and treatment nanoprobe and preparation method and application thereof
  • Hyperstable monodisperse fluorescent magnetic diagnosis and treatment nanoprobe and preparation method and application thereof

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Experimental program
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Effect test

Embodiment approach 1

[0034] (1) Preparation of magnetic particles (carboxylation): Weigh ferrous sulfate and ferric citrate with a molar ratio of 1:1, dissolve them in deionized water, stir at a speed of 600 rpm to make them well mixed; then add a certain amount of amount of ascorbic acid to make it compatible with Fe 3+ The molar ratio of ions was 1:3, and 0.5 M sodium hydroxide solution was used to adjust the pH value of the reaction solution to 9. After stirring for 120 min, the mixed solution was transferred to a hydrothermal reaction kettle and kept at 150 °C for 4 h; Dialyzed for 2 days to obtain MNPs-COOH suspension solution, which was then placed in a 4°C refrigerator for long-term storage;

[0035] (2) PEGylation of magnetic nanoparticles: Take 1 mL of the MNPs-COOH suspension solution, transfer it to a 10K ultrafiltration tube and centrifuge. The parameters of the refrigerated centrifuge are set to: 4 °C, 12000 rpm, 5 min; Make up to 7.2 of borate buffer to the original volume, the carb...

Embodiment approach 2

[0040] (1) Preparation of magnetic particles (carboxylation): Weigh ferrous sulfate and ferric citrate with a molar ratio of 1:2, dissolve them in deionized water, stir at a speed of 600 rpm to make them well mixed; then add a certain amount of amount of ascorbic acid to make it compatible with Fe 3+ The molar ratio of ions was 1:2, and 0.5 M sodium hydroxide solution was used to adjust the pH value of the reaction solution to 10. After stirring for 120 min, the mixed solution was transferred to a hydrothermal reaction kettle and kept at 150 °C for 4 h; Dialyzed for 2 days to obtain MNPs-COOH suspension solution, which was then placed in a 4°C refrigerator for long-term storage;

[0041] (2) PEGylation of magnetic nanoparticles: Take 1 mL of the MNPs-COOH suspension solution, transfer it to a 10K ultrafiltration tube and centrifuge. The parameters of the refrigerated centrifuge are set to: 4 °C, 12000 rpm, 5 min; Make up to 7.2 of borate buffer to the original volume, the car...

Embodiment approach 3

[0045] (1) Preparation of magnetic particles (carboxylation): Weigh ferrous sulfate and ferric citrate with a molar ratio of 1:1, dissolve them in deionized water, stir at a speed of 600 rpm to make them well mixed; then add a certain amount of amount of ascorbic acid to make it compatible with Fe 3+ The molar ratio of ions was 1:3, and the pH value of the reaction solution was adjusted to 12 with 0.5 M sodium hydroxide solution. After stirring for 120 min, the mixed solution was transferred to a hydrothermal reaction kettle, and the temperature was kept at 150 °C for 4 h; Dialyzed for 2 days to obtain MNPs-COOH suspension solution, which was then placed in a 4°C refrigerator for long-term storage;

[0046] (2) PEGylation of magnetic nanoparticles: Take 1 mL of the MNPs-COOH suspension solution, transfer it to a 10K ultrafiltration tube and centrifuge. The parameters of the refrigerated centrifuge are set to: 4 °C, 12000 rpm, 5 min; Make up to 7.2 of borate buffer to the orig...

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Abstract

The invention relates to a hyperstable monodisperse fluorescent magnetic diagnosis and treatment nanoprobe and a preparation method and application thereof. The nanoprobe comprises magnetic nano particles coating polyethylene glycol PEG and a CaCO3 coating layer doped with a photosensitizer, wherein a mass ratio of the magnetic nano particles to the CaCO3 coating layer is 10 to (1 to 3), and the load of the photosensitizer is 8.9-10.3 wt%; ascorbic acid is placed in an iron salt solution, a solution pH is adjusted to be 9 to 12, and through hydrothermal reaction, magnetic nano particle dispersion liquid is obtained; CaCl2 and ICG are added into the magnetic nano particle dispersion liquid, reaction is carried out while stirring, and then Na2CO3 and ICG are added for reaction for 24 hours to obtain a target product. The nanoprobe is used for bimodal imaging in an animal body, and has the advantages that blood circulation time is long, tumor location retention time is long, the medicinetargeted delivery efficiency is high, a preparation technology is simple, and the like.

Description

technical field [0001] The invention relates to the field of nano-medicine, in particular to an ultra-stable monodisperse fluorescent magnetic diagnosis and treatment nano-probe and a preparation method and application thereof. Background technique [0002] Gastric cancer is currently one of the most common digestive tract malignancies in the world, originating from epithelial malignancies, of which 40% of the world's patients are located in China. In recent years, great progress has been made in the treatment of gastric cancer in my country. For gastric cancer in different stages of early gastric cancer, advanced gastric cancer and advanced gastric cancer, different treatment methods have been adopted, such as surgical resection, chemotherapy, radiotherapy and radiotherapy / radiotherapy. However, these medical interventions have not achieved the desired expected therapeutic effect. [0003] With the development of modern medicine, nanotechnology and nanomaterials are increas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K49/12A61K49/08A61K49/18A61K41/00A61K9/14A61K47/02A61K47/10A61P35/00
CPCA61K9/143A61K9/146A61K41/0057A61K41/0071A61K49/0002A61K49/0034A61K49/0036A61K49/08A61K49/126A61K49/183A61K49/186A61P35/00
Inventor 刘党培刘岩磊
Owner 苏州纳葛诺斯生物科技有限公司
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