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Targeted superparamagnetic monodisperse nano-flower probe, preparation and application thereof

A nanoflower, monodisperse technology, applied in the field of nanoflower probes, can solve the problems of damage and side effects, and achieve the effect of accelerating the destruction of diseased cells, low cost, and excellent dispersion

Active Publication Date: 2017-02-08
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these treatment methods, while killing tumor cells, will also cause great damage to the normal body, with great side effects

Method used

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  • Targeted superparamagnetic monodisperse nano-flower probe, preparation and application thereof
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  • Targeted superparamagnetic monodisperse nano-flower probe, preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] A method for preparing a targeted superparamagnetic monodisperse nanoflower probe, mainly comprising the following steps:

[0049] (1) Fe 3 o 4 Preparation of nanoflowers:

[0050] a. Add 2mmol of FeCl 3 ·6H 2 O is placed in the mixed solution of ethylene glycol and diethylene glycol with a volume ratio of 1:15, ultrasonically stirred for 5 minutes to stop the transparent viscous yellow solution;

[0051] b.N 2 Add 2g of polyvinylpyrrolidone (PVP) under protection, mechanically stir at a speed of 150rpm, and keep warm at 120°C for 0.5h to obtain a yellow-brown transparent solution;

[0052] c.N 2 Under protection, add 50mmol of NaAc, increase the mechanical stirring speed to 300rpm, stir for 30min, then transfer to a polytetrafluoro reactor, set the temperature to 180°C, and keep it warm for 10h to obtain the product;

[0053] d. Wash the product three times alternately with ultrapure water and absolute ethanol, and after magnetic separation and purification, dil...

Embodiment 2

[0077] (1) Fe 3 o 4 Preparation of nanoflowers:

[0078] a. Add 8mmol of FeCl 3 ·6H 2 O is placed in the mixed solution of ethylene glycol and diethylene glycol with a volume ratio of 3:17, and ultrasonically stirred for 8 minutes to stop the transparent viscous yellow solution;

[0079] b.N 2 Add 8 g of polyvinylpyrrolidone (PVP) under protection, mechanically stir at a speed of 200 rpm, and keep warm at 125° C. for 1 hour to obtain a yellow-brown transparent solution;

[0080] c.N 2 Under protection, add 70mmol of NaAc, increase the mechanical stirring speed to 400rpm, and after stirring for 40min, transfer it to a polytetrafluoro reactor, set the temperature to 210°C, and keep it warm for 15h to obtain the product;

[0081] d. Wash the product three times alternately with ultrapure water and absolute ethanol, and after magnetic separation and purification, dilute to 50 mL with ultrapure water to obtain a magnetic nanoparticle dispersion, which is set aside;

[0082] ...

Embodiment 3

[0100] (1) Fe 3 o 4 Preparation of nanoflowers:

[0101] a. 15mmol of FeCl 3 ·6H 2 O is placed in the mixed solution of ethylene glycol and diethylene glycol with a volume ratio of 5:15, and ultrasonically stirred for 8 minutes to stop the transparent viscous yellow solution;

[0102] b.N 2 Add 10g of polyvinylpyrrolidone (PVP) under protection, mechanically stir at a speed of 250rpm, and keep warm at 130°C for 1.5h to obtain a yellow-brown transparent solution;

[0103] c.N 2 Under protection, add 100mmol of NaAc, increase the mechanical stirring speed to 500rpm, stir for 50min, then transfer to a polytetrafluoro reactor, set the temperature to 250°C, and keep it warm for 20h to obtain the product;

[0104] d. Wash the product three times alternately with ultrapure water and absolute ethanol, and after magnetic separation and purification, dilute to 50 mL with ultrapure water to obtain a magnetic nanoparticle dispersion, which is set aside;

[0105] (2) Fe 3 o 4 Prep...

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Abstract

The invention relates to a targeted superparamagnetic monodisperse nano-flower probe, the preparation and the application thereof. A nano-flower probe is composed of three parts, namely a magnetic gold-bearing particle nano-flower, a hyperbranched macromolecular HPG and a target molecule, wherein one end of the hyperbranched macromolecular HPG is provided with a mercapto group and a branched terminal. The nano-flower probe is prepared through the hot solvent method. That is, firstly, a Fe3O4 carrier is prepared. Secondly, a golden seed is loaded on the carrier, and then the magnetic gold-bearing particle nano-flower is obtained. Finally, the magnetic gold-bearing particle nano-flower is connected sequentially with the HPG and the target molecule. The above nano-probe is applied to the oncotherapy for animal in-vivo double-targeted imaging under the alternating magnetic field. Compared with the prior art, the targeted superparamagnetic monodisperse nano-flower probe is simple to prepare, low in cost, high in targeting efficiency, and small in oncotherapy side effect. Compared with the prior art, the targeted superparamagnetic monodisperse nano-flower probe has the advantages of simple preparation, low cost, high targeting efficiency, small oncotherapy side effect and the like.

Description

technical field [0001] The invention relates to the technical field of nanoflower probes, in particular to a targeted superparamagnetic monodisperse nanoflower probe and its preparation and application. Background technique [0002] With increasing morbidity and mortality, cancer has become the leading cause of death and a major public health problem in China. Statistics show that there were 4,292,000 new cancer cases and 2,814,000 cancer deaths in China in 2015. Lung cancer became the most common cancer and the leading cause of cancer death. Gastric cancer, esophageal cancer, and liver cancer are also common cancers, ranking among the top causes of cancer death. Since cancer has no designated location, it can occur in any part of the internal organs. The symptoms of cancer and precancerous lesions are insidious and non-specific, and the incubation period is long. Once it is found that it has reached an advanced stage or metastasized, the patient cannot be cured and dies ....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K49/12A61K47/61A61P35/00
CPCA61K41/0052A61K49/12
Inventor 高国尹婷张倩黄鹏崔大祥
Owner SHANGHAI JIAO TONG UNIV
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